LUO Donglan,QU Guangfan,LI Jiangkuo,et al.Isolation and Identification of Pathogens Causing Blueberry Rot During Postharvest Storage at Low Temperature and Screening of Biological Fungicides[J].Northern Horticulture,2024,(3):85-92.[doi:10.11937/bfyy.20232685]
蓝莓低温贮藏期致腐病原菌的分离鉴定及生物杀菌剂筛选
- Title:
- Isolation and Identification of Pathogens Causing Blueberry Rot During Postharvest Storage at Low Temperature and Screening of Biological Fungicides
- 文章编号:
- 1001-0009(2024)03-0085-08
- 分类号:
- S 663.9
- 文献标志码:
- A
- 摘要:
- 以‘粉蓝’蓝莓果实为试材，采用组织分离法、科赫法则、形态学鉴定及rRNA-ITS序列同源性分析方法，研究蓝莓果实主要致腐病原菌种类，并筛选适宜高效低毒的生物杀菌剂，以期为莓果实采后病害防控提供参考依据。结果表明：引起低温贮藏期蓝莓致腐的病原菌为灰葡萄孢菌（Botrytis cinerea）、链格孢菌（Alternaria alternata）、胶孢炭疽菌（Colletotrichum gloeosporioides）、棒状新拟盘多毛孢菌（Neopestalotiopsis clavispora）、小新壳梭孢菌（Neofusicoccum parvum），且首次发现胶孢炭疽菌（Colletotrichum gloeosporioides）是引起‘粉蓝’蓝莓果实致腐的优势致病菌。室内毒力试验发现，乙蒜素对胶孢炭疽菌表现出较强的抑菌活性，EC50值为123.16 mg·mL-1，其次为苦参碱、中生菌素、春雷霉素、丁子香酚，EC50值分别为231.23、519.55、546.29、865.26 mg·mL-1。因此，后期可将乙蒜素作为蓝莓后期田间防治的有效药剂。
- Abstract:
- Blueberry fruits(‘Powder Blue’) were used as experimental material，tissue isolation method，Koch′s law，morphological identification and rRNA-ITS sequence homology analysis methods were used to study the main rot-causing pathogen species of blueberry fruits and to screen for suitable high-efficiency and low-toxicity biocides，in order to provide reference for the prevention and control of berry fruits postharvest diseases.The results showed that the pathogens causing blueberry rot during low-temperature storage were Botrytis cinerea，Alternaria alternata，Colletotrichum gloeosporioides，Neopestalotiopsis clavispora and Neofusicoccum parvum.For the first time，Colletotrichum gloeosporioides was found to be the dominant pathogen causing fruit rot of ‘Powder Blue’ blueberry.The indoor toxicity test showed that ethylicin showed strong antibacterial activity against Colletotrichum gloeosporioides，with EC50 value of 123.16 mg·mL-1，followed by matrine，zhongshengmycin，kasugamycin and eugenol，with EC50 value of 231.23，519.55，546.29,865.26 mg·mL-1 respectively.Therefore，ethylicin could be used as an effective biocide for field control of blueberry in the later stage.
参考文献/References:
［1］LI D L，LI B，MA Y,et al.Polyphenols，anthocyanins，and flavonoids contents and the antioxidant capacity of various cultivars of highbush and half-high blueberries［J］.Journal of Food Composition and Analysis，2017，62:84-93.［2］吴超,杨富雁,郜帮丽.壳聚糖、山梨酸钾和ε-聚赖氨酸对蓝莓的复合涂膜保鲜效果［J］.北方园艺,2022(7):99-105.［3］HOSKIN R T，XIONG J，ESPOSITO D A,et al.Blueberry polyphenol-protein food ingredients：The impact of spray drying on the in vitro antioxidant activity，anti-inflammatory markers，glucose metabolism and fibroblast migration［J］.Food Chemistry，2019，280：187-194.［4］阳秀莲，王伟，林树花，等.蓝莓贮藏期病原菌的分离鉴定及植物粗提物抑菌效果研究［J］.食品科技，2018，43(5)：41-48.［5］王哲捷，徐十，王永平.贵州水果产业发展现状及高质量发展对策［J］.北方园艺，2022(6)：137-144.［6］ANGELETTI P，CASTAGNASSO H,MICELI E，et al.Effect of preharvest calcium applications on postharvest quality，softening and cell wall degradation of two blueberry (Vaccinium corymbosum) varieties［J］.Postharvest Biology and Technology，2010，58(2)：98-103.［7］吴文能，雷霁卿，李江阔，等.蓝莓采后病害病原菌分离鉴定及抑菌药剂筛选［J］.北方园艺，2019(7)：58-64.［8］王丹，张静，贾晓曼，等.蓝莓采后主要病原菌的分离鉴定及肉桂精油抑菌效果［J］.食品科学，2019，40(24)：167-172.［9］郜海燕，肖尚月，陈杭君，等.蓝莓采后主要病原真菌的分离鉴定与生物学特性研究［J］.农业机械学报，2017，48(5)：327-334.［10］崔学强，李秀玲，范继征，等.兜兰萎蔫病病原菌的分离与鉴定［J］.西南农业学报，2020，33(12)：2846-2851.［11］瞿光凡，巴良杰，雷霁卿，等.茉莉酸甲酯对脆红李采后病原菌的抑制作用［J］.食品研究与开发，2022，43(24)：95-101.［12］姜莉莉，王钦超，宗晓娟，等.草莓果实病原菌的分离鉴定及对12种杀菌剂的敏感性［J］.山东农业科学，2022，54(2)：129-133.［13］FU X D，LI X A，ALI M,et al.Methionine sulfoxide reductase B5 plays vital roles in tomato fruit defense response against Botrytis cinerea induced by methyl jasmonate［J］.Postharvest Biology and Technology，2023，196：112165.［14］FAN L，WEI Y Y，CHEN Y,et al.Epinecidin-1，a marine antifungal peptide，inhibits Botrytis cinerea and delays gray mold in postharvest peaches［J］.Food Chemistry，2023，403：134419.［15］JIANG B，LIU R L，FANG X J,et al.Botrytis cinerea infection affects wax composition，content and gene expression in blueberry fruit［J］.Postharvest Biology and Technology，2022，192：112020.［16］WANG L Y，HU J P，LI D S,et al.Isolation and control of Botrytis cinerea in postharvest green pepper fruit［J］.Scientia Horticulturae，2022，302：111159.［17］JAVANMARDI Z，KOUSHESH S M，NOURBAKHSH H,et al.Efficiency of nanoemulsion of essential oils to control Botrytis cinerea on strawberry surface and prolong fruit shelf life［J］.International Journal of Food Microbiology，2023，384：109979.［18］LIU Y，LEI X M，DENG B,et al.Methionine enhances disease resistance of jujube fruit against postharvest black spot rot by activating lignin biosynthesis［J］.Postharvest Biology and Technology，2022(190)：111935.［19］LI Y C，MA Y Y，ZHANG T T,et al.Exogenous polyamines enhance resistance to Alternaria alternata by modulating redox homeostasis in apricot fruit［J］.Food Chemistry，2019，301(15)：125303.［20］向妙莲，吴帆，李树成，等.外源褪黑素调控活性氧代谢诱导梨果实抗采后黑斑病［J］.园艺学报，2022，49(5)：1102-1110.［21］吴玉珠，陈娜，胡军华，等.青脆李枯萎病致病菌的分离鉴定及药剂筛选［J］.湖南农业大学学报(自然科学版)，2022，48(3)：298-304.［22］王正贤.广西玉林市番石榴果实病害的病原菌鉴定及黑斑病菌的生物学特性研究［D］.南宁：广西大学，2021.［23］吴望，吴玉珠，胡军华，等.‘沃柑’果斑病病原菌鉴定及防治药剂筛选［J］.果树学报，2021，38(3)：385-393.［24］CHEN X Y L，HE B Y，LI H X,et al.First report of black rot on walnut fruits caused by Neofusicoccum parvum in China［J］.Plant Disease，2019，103(12)：3275.［25］ZHOU D B，JING T，CHEN Y F,et al.Biocontrol potential of a newly isolated Streptomyces sp.HSL-9B from mangrove forest on postharvest anthracnose of mango fruit caused by Colletotrichum gloeosporioides［J］.Food Control，2022，135：108836.［26］EKABOTE S D，NARAYANSWAMY P.Bio-efficacy of various fungicides against Clletotrichum gloeosporioides causing anthracnose on pomegranate fruit［J］.Acta Horticulturae，2019，1254：253-256.［27］徐成楠，王亚南，胡同乐，等.蓝莓炭疽病病原菌鉴定及致病性测定［J］.中国农业科学，2014，47(20)：3992-3998.［28］张国辉，刘德波，宋盛英，等.黔东南州蓝莓叶部病害的种类调查和病原鉴定［J］.中国森林病虫，2017，36(2)：42-46.［29］刘洪坤.蓝莓炭疽病的药剂防治试验［J］.中国南方果树，2017，46(2)：163-165.［30］张伟，于云政，刘洁，等.生物药剂对花生叶斑病的防治研究［J］.花生学报，2016，45(2)：62-67.［31］胡容平，石军，林立金，等.猕猴桃溃疡病高效防治药剂的筛选及田间应用评价［J］.中国农学通报，2020，36(6)：112-116.［32］李慧，刘保军，吴琼，等.3种生防制剂对棉花红腐病和立枯病的防效评价［J］.新疆农业科学，2020，57(4)：694-704.［33］赵岩，白田田，顾欣，等.宁夏地区欧李根癌病病原菌鉴定及防治药剂筛选［J］.东北林业大学学报，2023，51(3)：130-135.
相似文献/References:
[1]沈清清,刘 芳,胡 彦.药用植物根腐病病原菌研究进展[J].北方园艺,2014,38(11):187.
SHEN Qing-qing,LIU Fang,HU Yan.Research Progress of Pathogens of Root Rot Disease on Medicinal Plants[J].Northern Horticulture,2014,38(3):187.
[2]王晓静,马文平,倪志婧.“玉金香”甜瓜采后主要病害病原菌生物学特性研究[J].北方园艺,2013,37(14):128.
WANG Xiao-jing,MA Wen-ping,NI Zhi-jing.Biological Characteristic of the Main Postharvest Diseases Pathogen of Melon Cultivar ‘Yujinxiang’[J].Northern Horticulture,2013,37(3):128.
[3]王中武,奚广生,战任权.玉竹叶斑病病原鉴定及生物学特性研究[J].北方园艺,2014,38(08):112.
WANG Zhong-wu,XI Guang-sheng,ZHAN Ren-quan.Research on Pathogens Determination and Biological Characteristics of Leaf Spot in Odoratum[J].Northern Horticulture,2014,38(3):112.
[4]何玉会,白庆荣,杜佳朋,等.甜瓜枯萎病的病原鉴定及药剂筛选研究[J].北方园艺,2014,38(03):114.
HE Yu-hui,BAI Qing-rong,DU Jia-peng,et al.Study on Pathogen Identification and Screening of Control Fungicides of Muskmelon Fusarium Wilt[J].Northern Horticulture,2014,38(3):114.
[5]王雪,王晶,高洁,等.五味子褐斑病病原菌生物学特性研究[J].北方园艺,2013,37(07):142.
WANG Xue,WANG Jing,GAO Jie,et al.Biological Characteristics of the Pathogen and Fungicides Screening in Laboratory for Schisandra chinensis Brown Leaf Spot Caused by Phoma glomerata[J].Northern Horticulture,2013,37(3):142.
[6]任玉锋,唐艳军.三种保鲜剂及其复合物对灵武长枣采后主要病原菌菌丝生长的影响[J].北方园艺,2012,36(16):159.
REN Yu-feng,TANG Yan-jun.Effects of Three Preservatives and Their Combinations on Hyphal Growth of Major Pathogenic Fungi in Post-harvest Zizphus jujube Mill cv. ‘Lingwuchangzao’[J].Northern Horticulture,2012,36(3):159.
[7]李红梅,林燕飞,刘昌镇,等.纳米银预处理对麝香百合切花的保鲜效应研究[J].北方园艺,2012,36(08):166.
LI Hong-mei,LIN Yan-fei,LIU Chang-zhen,et al.Freshness-preserving Effects of Nano-silver Pre-treatments on Cut Lily Flowers[J].Northern Horticulture,2012,36(3):166.
[8]李兵兵,温志强.银耳杨梅霜病发病机理及其病原菌抗菌谱的测定[J].北方园艺,2015,39(24):114.[doi:10.11937/bfyy.201524032]
LI Bingbing,WEN Zhiqiang.Pathogenicity Mechanism of Yang Mei Shuang Disease on Tremella fuciformis and Inhibition Spectrum of the Pathogenic Bacterium[J].Northern Horticulture,2015,39(3):114.[doi:10.11937/bfyy.201524032]
[9]张玉洁.,张志信l.,李红超..柑橘炭疽病的病原鉴定和综合防治措施[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102054]
ZHANG Yu-jie.,ZHANG Zhi-xin.,LI Hong-chao..Identification and Prevention Method of Pathogenic Fungi of Citrus Anthracnose[J].Northern Horticulture,2011,35(3):0.[doi:10.11937/bfyy.201102054]
[10]王 芳,黄 碧 龙,王 艳.菊花褐斑病发生规律及防治研究[J].北方园艺,2001,25(01):0.[doi:10.11937/bfyy.200101024]
[J].Northern Horticulture,2001,25(3):0.[doi:10.11937/bfyy.200101024]
备注/Memo
第一作者简介：罗冬兰（1991-），女，硕士，副教授，现主要从事农产品贮藏与加工等研究工作。E-mail：904960670@qq.com.责任作者：曹森（1988-），男，硕士，教授，现主要从事农产品质量安全与品质分析等研究工作。E-mail：cs5638myself@126.com.基金项目：贵州省科技支撑计划资助项目（黔科合支撑［2021］一般222）。收稿日期：2023-08-07