KANG Shengwan,ZHOU Qiwu,LIU Di,et al.Effects of Controllable Environmental Factors on the Mycelial Growth of Ganoderma leucocontextum[J].Northern Horticulture,2023,(23):107-114.[doi:10.11937/bfyy.20232355]
可控环境因子对白肉灵芝菌丝生长的影响
- Title:
- Effects of Controllable Environmental Factors on the Mycelial Growth of Ganoderma leucocontextum
- 文章编号:
- 1001-0009(2023)23-0107-08
- Keywords:
- Ganoderma leucocontextum; mycelial growth; controlled environmental condition; orthogonal experiment
- 分类号:
- S 646.9
- 文献标志码:
- A
- 摘要:
- 以白肉灵芝为试材，采用单因素与L16（45）正交实验的方法，研究了不同温度梯度、琼脂浓度、pH、光照时间及培养皿大小对该菇菌丝生长的影响，以期为其后续开发利用提供参考依据。结果表明：白肉灵芝菌丝在温度范围为15~35 ℃以及其他可控环境因子的所有处理上均能生长，其中在温度27~29 ℃、琼脂浓度2.0%~3.0%、酸碱度5.00~5.25、光照时间0 h·d-1的各单因素处理下该菇菌丝萌发快，菌落浓密健壮，生长最快，而氧气对其无显著性影响。在温度25 ℃、琼脂浓度2.5%、酸碱度5.00、光照时间0 h·d-1、培养皿大小90 mm的可控环境因子最佳组合下，白肉灵芝菌丝生长速度最快，达到（9.88±0.51）mm·d-1。综上所述，白肉灵芝菌丝生长受不同可控环境因素的影响较大，且各因素之间有较大的交互作用。
- Abstract:
- Taking Ganoderma leucocontextum as the test material，the effects of different temperature gradients，agar concentration，pH，light time and petri dish size on the mycelial growth of Ganoderma leucocontextum were investigated by using the method of one-way and orthogonal experiments with L16 (45),in order to provide reference for its subsequent exploitation.The results showed that the mycelium of G.leucocontextum could grow on all treatments with a temperature range of 15-35 ℃ and other controllable environmental factors，among which the mycelium germinated quickly，the colony was dense and robust，and the growth was the fastest under the single-factor treatment of temperature 27-29 ℃，agar concentration of 2.0%-3.0%，pH 5.00-5.25 and light time of 0 h·d-1，while oxygen had no significant effect on it.Under the best combination of controllable environmental factors such as temperature 25 ℃，agar concentration 2.5%，pH 5.00，light time 0 h·d-1，and petri dish size of 90 mm，the mycelial growth rate of G.leucocontextum was the fastest，reaching (9.88±0.51)mm·d-1.In summary，the growth of G.leucocontextum was greatly affected by different controllable environmental factors，and there was a greater interaction between various factors.
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备注/Memo
第一作者简介：康晟菀(2000-)，女，硕士，助教，现主要从事白肉灵芝的驯化与开发等研究工作。E-mail：1749133926@qq.com.责任作者：周会明（1984-）,男，硕士，副教授，现主要从事大型真菌种质资源驯化栽培等研究工作。E-mail：632243530@qq.com.基金项目：临沧市科技创新人才培养资助项目（202204AC100001-RC01）；中央引导地方科技发展资金资助项目（202207AB110007）。收稿日期：2023-07-12