YANG Weimin,YAN Junfeng,ZHAI Yuanyuan,et al.Effects of BoEPXA2 Gene on Seed Germination of Kale Under Abiotic Stress[J].Northern Horticulture,2024,(1):60-66.[doi:10.11937/bfyy.20232159]
非生物胁迫下BoEPXA2基因对羽衣甘蓝种子萌发的影响
- Title:
- Effects of BoEPXA2 Gene on Seed Germination of Kale Under Abiotic Stress
- 文章编号:
- 1001-0009(2024)01-0060-07
- Keywords:
- abiotic stress; seed germination; enzyme activity; tolerance; kale
- 分类号:
- S 635.1
- 文献标志码:
- A
- 摘要:
- 以野生型、BoEXPA2基因过表达和BoEXPA2基因抑制表达的羽衣甘蓝为试材，采用无机试剂NaCl、蔗糖（sucrose）和甘露醇（mannitol）分别设定低浓度和高浓度NaCl（100 mmol·L-1和200 mmol·L-1）、蔗糖（150 mmol·L-1和250 mmol·L-1）和甘露醇（200 mmol·L-1或400 mmol·L-1），分析在不同的胁迫下3个株系种子萌发情况，并测定各基因型不同胁迫下抗氧化酶活性，MDA含量及BoEXPA2的表达,以期探讨羽衣甘蓝BoEXPA2基因在非生物胁迫下对种子萌发的影响。结果表明：在非生物胁迫下，与野生型种子相比，BoEXPA2抑制表达种子的发芽率显著降低，而BoEXPA2过表达系的发芽率显著提高。在干旱胁迫和盐胁迫下，3种基因型种子的超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量均有增加，但是BoEXPA2过表达株系种子的SOD、POD、CAT 3种酶活性远高于对照和抑制表达株系，而MDA含量低于对照和抑制表达株系；当种子被NaCl、蔗糖和甘露醇处理时，随着含量的增加，BoEXPA2的表达程度均会随之降低，这表明非生物胁迫抑制了发芽种子中BoEXPA2的表达。
- Abstract:
- The wild type，BoEXPA2 gene overexpression，and BoEXPA2 gene suppressed expression of kale were used as experimental materials.Three inorganic reagents，NaCl，sucrose，and mannitol were used.Low and high concentrations of NaCl (100 mmol·L-1 and 200 mmol·L-1)，sucrose (150 mmol·L-1 and 250 mmol·L-1)，and mannitol (200 mmol·L-1and 400 mmol·L-1) groups were set，respectively.The seed germination of three strains under different stresses was analyzed，and antioxidant enzyme activity，MDA content，and BoEXPA2 expression were measured for each genotype under different stresses,in order to explore the effect of BoEXPA2 gene on seed germination of kale under abiotic stress.The results showed that under abiotic stress，compared with wild type seeds，the germination rate of BoEXPA2 inhibited expression seeds was significantly reduced，while the germination rate of BoEXPA2 overexpression lines was significantly increased.Under drought stress and salt stress，the activities of superoxide dismutase (SOD)，peroxidase (POD)，catalase (CAT) and the content of malondialdehyde (MDA) in the seeds of the three genotypes were increased，but the activities of SOD，POD，CAT in the seeds of the BoEXPA2 overexpression strain were far higher than those of the control and suppression expression strains，while the activities of MDA were lower than those of the control and suppression expression strains.When the seeds were treated with NaCl，sucrose and mannitol，the expression of BoEXPA2 decreased with the increase of their content.The results indicated that abiotic stress inhibits the expression of BoEXPA2 in germinated seeds.
参考文献/References:
［1］THOMPSON D S，ISLAM A.Plant cell wall hydration and plant physiology：An exploration of the consequences of direct effects of water deficit on the plant cell wall［J］.Plants (Basel，Switzerland)，2021，10(7)：1263.［2］丁安明，陈志华，杨懿德，等.NtabEXPA12基因过表达对烟草叶片发育及抗逆性的影响［J］.中国烟草科学，2021，42(4):58-66.［3］SAMALOVA M，MELNIKAVA A，ELSAYAD K，et al.Hormone-regulated expansins：Expression，localization，and cell wall biomechanics in Arabidopsis root growth［J］.Plant Physiol，2023，5(5):1-20.［4］CHEN Y，ZHANG B，LI C，et al.A comprehensive expression analysis of the expansin gene family in potato (Solanum tuberosum) discloses stress-responsive expansin-like B genes for drought and heat tolerances［J］.PLoS One，2019，14(7):e0219837.［5］LENK I，FISHER L，VICKERS M，et al.Transcriptional and metabolomic analyses indicate that cell wall properties are associated with drought tolerance in Brachypodium distachyon［J］.Int J Mol Sci，2019，20(7):1758.［6］HAN Y,CHEN Y，YIN S，et al.Over-expression of TaEXPB23，a wheat expansin gene，improves oxidative stress tolerance in transgenic tobacco plants［J］.J Plant Physiol，2015，173(1)：62-71.［7］ZHANG B，CHANG L，SUN W，et al.Overexpression of an expansin-like gene，GhEXLB2 enhanced drought tolerance in cotton［J］.Plant Physiology and Biochemistry:PPB，2021，162:468-475.［8］杨锐霞.柳树扩展蛋白基因鉴定及耐盐功能研究［D］.北京:北京林业大学，2021.［9］NAGAR S，SINGH V P，ARORA A，et al.Understanding the role of gibberellic acid and paclobutrazol in terminal heat stress tolerance in wheat［J］.Front Plant Sci，2021，12(8):1557.［10］PENG L，XU N，WANG Q Y，et al.Overexpression of paralogues of the wheat expansin gene TaEXPA8 improves low-temperature tolerance in Arabidopsis［J］.Plant biology，2019，21(6):1-13.［11］梁绮文.干旱胁迫下普通烟草类扩展蛋白NtEXLA2和NtEXLA4基因功能分析［D］.昆明:云南农业大学，2022.［12］ABBASI A，MALEKPOUR M，SOBHANVERDI S.The Arabidopsis expansin gene (AtEXPA18) is capable to ameliorate drought stress tolerance in transgenic tobacco plants［J］.Mol Biol Rep，2021，48(8):5913-5922.［13］ANA C M，CRISTIANO L，BRUNA M，et al.Ectopic expression of an expansin-like B gene from wild Arachis enhances tolerance to both abiotic and biotic stresses［J］.Plant J，2021,107(6):1681-1696.［14］GALL H，PHILIPPE F，DOMON J M，et al.Cell wall metabolism in response to abiotic stress［J］.Plants，2015，4(1):112-166.［15］XU Q，XU X，SHI Y，et al.Transgenic tobacco plants overexpressing a grass Ppexp1 gene exhibit enhanced tolerance to heat stress［J］.PLoS One，2014,9(7):e100792.［16］WU Y，THORNE E T，SHARP R E，et al.Modification of expansin transcript levels in the maize primary root at low water potentials［J］.Plant Physiol，2001，126:1471-1479.［17］LEE D K，AHN J H，SONG S K，et al.Expression of an expansin gene is correlated with root elongation in soybean［J］.Plant Physiol，2003，131(3):985-997.［18］SHAO Y，FENG X，NAKAHARA H，et al.Apical-root apoplastic acidification affects cell wall extensibility in wheat under salinity stress［J］.Plant Physiol，2021,173(4)：1850-1861.［19］JADAMBA C，KANG K，PAEK N C，et al.Overexpression of rice expansin7 (Osexpa7) confers enhanced tolerance to salt stress in rice［J］.Int J Mol Sci，2020，21(2):454.［20］MAROWA P，DING A，KONG Y.Expansins：Roles in plant growth and potential applications in crop improvement［J］.Plant Cell Rep，2016(35):949-965.［21］高俊凤.植物生理学实验指导［M］.北京:高等教育出版社,2006.［22］王广龙,刘梦婷,王云鹏,等.大蒜扩展蛋白基因AsEXPA8的分离及其参与渗透胁迫响应的分析［J］.核农学报，2022，36(12):2349-2357.［23］张安,曹清河,周志林,等.植物细胞壁松弛因子:Expansin研究进展［J］.江苏农业科学,2013,41(6):11-13.［24］李紫帅,张喜年,柳文杰,等.保水剂对砂生槐幼苗生长及生理的影响［J］.北方园艺,2023(5):53-60.［25］袁牧,王昌留,王一斐,等.超氧化物歧化酶的研究进展［J］.中国组织化学与细胞化学杂志，2016，25(6):550-558.［26］LI L，YI H.Effect of sulfur dioxide on ROS production，gene expression and antioxidant enzyme activity in Arabidopsis plants［J］.Plant Physiol Bioch，2012，58:46-53.［27］王永吉,郝小玲,周佳佳,等.外源甜菜碱对干旱胁迫下紫花苜蓿种子萌发与幼苗生长的影响［J］.北方园艺，2022(15):51-57.［28］LI F，XING S，GUO Q，et al.Drought tolerance through over-expression of the expansin gene TaEXPB23 in transgenic tobacco［J］.J Plant Physiol，2011，168(9):960-966.［29］ZHANG H，LIU H，YANG R，et al.Over-expression of PttEXPA8 gene showed various resistances to diverse stresses［J］.Int J Biol Macromol，2019，130:50-57.
相似文献/References:
[1]傅巧娟,李春楠,陈一,等.我国一串红的研究进展[J].北方园艺,2013,37(04):192.
[2]石秋梅,邓翻云,吴敏言,等.罗布麻和大叶白麻种子萌发及幼苗生长耐盐性研究[J].北方园艺,2014,38(12):128.
SHI Qiu-mei,DENG Fan-yun,WU Min-yan,et al. Study on Salt Tolerance of Apocynum venetum Linn.and Poacynum hendersonii(Hook.f.) Woodson at Stages of Seed Germination and Seedlings Growth[J].Northern Horticulture,2014,38(1):128.
[3]金 兰,陈 志.青海肋果沙棘和中国沙棘种子萌发特性比较研究[J].北方园艺,2014,38(13):39.
JIN Lan,CHEN Zhi.Comparative Study on Germination Characteristics of Hippophae neurocarpa and Hippophae rhamnoides Seeds[J].Northern Horticulture,2014,38(1):39.
[4]梁 芳,衣采洁.不同浸种处理对小冠花及蛇鞭菊种子萌发的影响[J].北方园艺,2014,38(11):62.
LIANG Fang,YI Cai-jie.Infulence of Different Processing Methods on the Seed Germination of Coronilla varia and Liatris spieata [J].Northern Horticulture,2014,38(1):62.
[5]崔兴国.不同浓度碳酸氢钠胁迫对圆叶牵牛种子发芽的影响[J].北方园艺,2014,38(11):74.
CUI Xing-guo.Effect of Different Concentration of NaHCO3 Stress on Seed Germination of Pharbitis purpurea(L.)Voigt[J].Northern Horticulture,2014,38(1):74.
[6]常青山,张利霞,郑轶琦,等.盐胁迫下钙离子对苜蓿种子萌发的影响[J].北方园艺,2014,38(10):64.
CHANG Qing-shan,ZHANG Li-xia,ZHENG Yi-qi,et al.Effects of Ca2+?on Seed Germination Characteristics of Medicago sativa Under Salt Stress[J].Northern Horticulture,2014,38(1):64.
[7]张 娇,张大治,马 艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014,38(14):65.
ZHANG Jiao,ZHANG Da-zhi,MA Yan,et al.Effect of NaCl Simulation of Salt Stress on Seed Germination and Seedling Growth of Ammopiptanthus mongolicus[J].Northern Horticulture,2014,38(1):65.
[8]杨 飞,郭海波,吴 菊,等.NaCl胁迫对白菜种子萌发及幼苗生长的影响[J].北方园艺,2014,38(01):26.
YANG Fei,GUO Hai-bo,WU Ju,et al.Effect of NaCl Stress on the Seed Germination and Seedling Growth of Chinese Cabbage Cultivars[J].Northern Horticulture,2014,38(1):26.
[9]张雷,李永洁,王雅茹,等.海水胁迫对半枝莲种子萌发的影响[J].北方园艺,2013,37(13):69.
ZHANG Lei,LI Yong-jie,WANG Ya-ru,et al.Effect of Seawater Stress on Scutellaria barbata Seed Germination[J].Northern Horticulture,2013,37(1):69.
[10]何会流.外源水杨酸对盐胁迫下凤仙花种子萌发特性的影响[J].北方园艺,2013,37(13):75.
HE Hui-liu.Effect of Exogenous Salicylic Acid on the Seeds Germination Characters of Impatiens balsamina L.Under NaCl Stress[J].Northern Horticulture,2013,37(1):75.
备注/Memo
第一作者简介：杨伟民（1973-），男，本科，工程师，现主要从事园林绿化养护等研究工作。E-mail：75161311@qq.com.责任作者：李鸿雁（1967-），女，硕士，教授，现主要从事园林等研究工作。E-mail：1248762303@qq.com.基金项目：河南省科技攻关资助项目（212102110005）。收稿日期：2023-06-25