LUO Qian,ZHANG Fengying,LAN Shichao,et al.Identification and Biological Characteristics of Cherry Brown Rot[J].Northern Horticulture,2023,(24):24-29.[doi:10.11937/bfyy.20231886]
樱桃褐腐病菌鉴定及生物学特性研究
- Title:
- Identification and Biological Characteristics of Cherry Brown Rot
- 文章编号:
- 1001-0009(2023)24-0024-06
- 分类号:
- S 662.5
- 文献标志码:
- A
- 摘要:
- 以贵州樱桃褐腐病果实为试材,根据柯赫氏法则验证所分离病原菌的致病性,结合形态学及分子生物学鉴定病原菌种类并探究其生物学特性,旨在为樱桃褐腐病的科学精准防控提供参考依据。结果表明:分离得到病原菌菌株YTBH10,菌落为灰褐色或灰白色,产生大量灰褐色孢子,呈同心轮状,分生孢子呈链状排列;ITS序列构建系统发育树分析显示该菌株属于链核盘菌属;病原菌回接试验证明其致病性。引起贵州樱桃褐腐病的病原菌是美澳型核果褐腐病菌(Monilinia fructicola),该病原菌在pH 3~13范围内均能生长,适合中性偏酸性环境,菌丝生长最适温度为25 ℃。
- Abstract:
- Taking the diseased fruit of cherry brown rot from Guizhou as experimental materials,the pathogen causing cherry brown rot was verified based on Koch postulates,and identified based on morphological and physiological,and their biological characteristics was explored,in order to provide reference for the accurate prevention and control of cherry brown rot.The results showed that the isolated pathogenic strain YTBH10 had a grey-brown or grey-white colony with a large number grey-brown spores which were concentric wheel shape and conidia were arranged in chain shape.Phylogenetic tree based on ITS indicated that the strain belonged to Monilinia.The candidate isolate could cause disease after experiment on cherry fruits.Therefore,the pathogen causing brown rot in Guizhou cherry was identified as Monilinia fructicola.The pathogen could grow in the pH range of 3-13 and prefered a neutral to slightly acidic environment with the optimal temperature for mycelial growth being 25 ℃.
参考文献/References:
[1]李孝绒,侯黔东,姜昱雯,等.‘玛瑙红’樱桃生长素响应因子CpARF7基因克隆及表达分析[J].北方园艺,2021(19):7-14.[2]田文杰,乔光,文晓鹏,等.贵州主栽樱桃品种的植原体分子检测及鉴定[J].种子,2022,41(2):18-24.[3]盛玉,陈红莉,谢庆梅,等.樱桃褐腐病测报及其生产防治关键技术[J].园艺与种苗,2021,41(2):59-61.[4]郗良卿,张士凯,陈雨诗,等.“美早”甜樱桃褐腐病病原菌的分离及内生拮抗细菌的筛选鉴定[J].食品与发酵工业,2020,46(8):85-91.[5]岳文秀.乌当区樱桃褐腐病的发生特点与防控技术[J].农技服务,2020,37(4):77-78.[6]朱小琼,段维军,胡勐郡,等.核果和仁果褐腐病病原菌研究进展[J].菌物学报,2022,41(3):331-348.[7]张艳婷,仇智灵,李阿根,等.浙江省樱桃褐腐病病原菌种类及其对常见药剂的抗性检测[J].果树学报,2020,37(9):1394-1403.[8]董怡,李阿根,毛程鑫,等.樱桃褐腐病菌对啶酰菌胺的敏感性及其对4种琥珀酸脱氢酶抑制剂的交互抗性[J].农药学学报,2022,24(2):298-305.[9]万艳,王方,郑红军,等.贵州毕节樱桃植原体病害研究初报[J].贵州林业科技,2020,48(4):34-38.[10]罗克明,穆雪,王爱华,等.贵州大樱桃产业发展现状与对策[J].耕作与栽培,2022,42(1):138-140.[11]吕昭龙,蒋晶晶,李继平,等.苹果果实褐腐病病原菌分离鉴定[J].西北农业学报,2023,32(3):488-494.[12]李志伟,汪少丽,刘保友,等.山东桃褐腐病病原菌种群鉴定及致病性分析[J].果树学报,2023,40(2):327-339.[13]ZHU X Q,NIU C W,CHEN X Y,et al.Monilinia species associated with brown rot of cultivated apple and pear fruit in China[J].Plant Disease,2016,100(11):2240-2250.[14]IOOS R,FREY P.Genomic variation within Monilinia laxa,M.fructigena and M.fructicola,and application to species identification by PCR[J].European Journal of Plant Pathology,2000,106(4):373-378.[15]WANG J R,GUO L Y,XIAO C L,et al.Detection and identification of six Monilinia spp.causing brown rot using TaqMan real-time PCR from pure cultures and infected apple fruit[J].Plant Disease,2018,102(8):1527-1533.[16]牛程旺,王静茹,朱小琼,等.新疆野果林褐腐病菌的种类[J].菌物学报,2016,35(12):1514-1525.[17]HU M J,COX K D,SCHNABEL G,et al.Monilinia species causing brown rot of peach in China[J].PLoS One,2011,6(9):e24990.[18]LANE C R.A synoptic key for differentiation of Monilinia fructicola,M.fructigena and M.laxa,based on examination of cultural characters[J].EPPO Bulletin,2002,32(3):489-493.[19]OGAWA J,ZEHR E,BIRD G,et al.Compendium of stone fruit diseases[M].USA:APS Press,1995.[20]de MICCOLIS ANGELINI R M,LANDI L,RAGUSEO C,et al.Tracking of diversity and evolution in the brown rot fungi Monilinia fructicola,Monilinia fructigena,and Monilinia laxa[J].Front Microbiol,2022(13):854852.[21]安星宇,李海,黄露,等.织金县玛瑙红樱桃褐腐病病原菌的鉴定[J].农业与技术,2021,41(15):34-37.[22]de MICCOLIS ANGELINI R M,ROMANAZZI G,POLLASTRO S,et al.New high-quality draft genome of the brown rot fungal pathogen Monilinia fructicola[J].Genome Biol Evol,2019,11(10):2850-2855.[23]ANGELI S S,de MIO L L M,AMORIM L.Comparative analysis of Monilinia fructicola and M.laxa isolates from Brazil:Monocyclic components of peach brown rot[J].Ciencia Rural,2017,47(6):e20160300.[24]刘志恒,白海涛,杨红,等.大樱桃褐腐病菌生物学特性研究[J].果树学报,2012,29(3):423-427,523.[25]纪兆林,朱薇,谈彬,等.我国不同产区桃褐腐病菌生物学特性研究[J].中国南方果树,2018,47(5):101-107.
备注/Memo
第一作者简介:罗倩(1979-),女,博士,教授,现主要从事植物保护和化学生态学等研究工作。E-mail:13929346@qq.com.基金项目:教育厅自然科学研究资助项目(黔教合KY字[2021]235)。收稿日期:2023-06-01