[1]何义利,丁建莉,魏丹,等.设施闷棚与蔬菜秸秆原位还田对土壤真菌群落的影响[J].北方园艺,2023,(23):83-91.[doi:10.11937/bfyy.20231467]
　HE Yili,DING Jianli,WEI Dan,et al.Effects of Greenhouse and Vegetable Straw Returning in Situ on Soil Fungal Community[J].Northern Horticulture,2023,(23):83-91.[doi:10.11937/bfyy.20231467]
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设施闷棚与蔬菜秸秆原位还田对土壤真菌群落的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年23 页码: 83-91 栏目: 出版日期: 2023-12-15
Title:
Effects of Greenhouse and Vegetable Straw Returning in Situ on Soil Fungal Community
文章编号:
1001-0009(2023)23-0083-09
作者:
何义利1; 2; 丁建莉2; 魏丹1; 2; 李沛1; 2; 黄子鹏1; 2; 张祎1; 2
（1.东北农业大学 资源与环境学院，黑龙江 哈尔滨 150030；2.北京市农林科学院 植物营养与资源环境研究所，北京 100097）
Author(s):
HE Yili1; 2; DING Jianli2; WEI Dan1; 2; LI Pei1; 2; HUANG Zipeng1; 2; ZHANG Yi1; 2
(1.College of Resources and Environment，Northeast Agricultural University，Harbin，Heilongjiang 150030；2.Institute of Plant Nutrition and Resource Environment，Beijing Academy of Agricultural and Forestry Sciences，Beijing 100097)
关键词:
设施闷棚; 蔬菜秸秆; 原位还田; 真菌群落; 群落功能
Keywords:
facility stuffy shed; vegetable straw; return in situ; fungal community; community function
分类号:
X 172
DOI:
10.11937/bfyy.20231467
文献标志码:
A
摘要:
以辣椒秸秆为试材，采用高通量测序方法分析土壤真菌的ITS序列，试验设置3个处理分别为秸秆不还田（CK）、秸秆还田（NK）和秸秆还田加菌剂（TJ），测定了土壤水溶性有机碳、硝态氮、铵态氮、速效钾以及土壤真菌群落结构等相关指标，研究了设施蔬菜秸秆原位还田对土壤真菌群落结构的影响，以期阐明土壤生态群落结构对蔬菜秸秆还田的响应关系。结果表明：与闷棚前土壤相比，秸秆还田加菌剂显著提高了电导率，同时土壤水溶性有机碳、硝态氮、铵态氮和速效钾含量分别提高了58.25%、219.50%、34.59%和101.70%。秸秆还田改变了土壤真菌群落组成，与秸秆不还田相比，秸秆还田使得优势菌门子囊菌门（Ascomycota）的相对丰度提高了9.17%，优势菌属被孢霉属（Mortierella）的相对丰度降低了0.17%；但与闷棚前相比，闷棚使得优势菌属被孢霉属（Mortierella）的相对丰度显著降低了8.29%。土壤的理化因子对所有样品中真菌群落多样性的影响大小顺序为硝态氮>铵态氮>有效磷>EC值>pH>水溶性有机碳>速效钾。设施蔬菜秸秆还田显著增加土壤水溶性有机碳、硝态氮、铵态氮、速效钾含量，并且秸秆还田添加腐熟菌剂处理增加更显著。蔬菜秸秆原位还田改变了真菌群落组成，但并没有增加病原菌的含量，植株生长过程中也没有病虫害发生，表明秸秆原位还田与闷棚相结合是科学的农艺措施。
Abstract:
The pepper straw was used as the test material，and the ITS sequence of soil fungi was analyzed by high-throughput sequencing.The effects of in-situ returning of greenhouse vegetable straw on soil fungal community structure were studied,in order to clarify the response of soil ecological community structure to vegetable straw returning.Three treatments were set up,no straw returning (CK)，straw returning (NK) and straw returning with microbial agent (TJ).The soil water-soluble organic carbon，nitrate nitrogen，ammonium nitrogen，available potassium and soil fungal community structure were measured.The results showed that compared with the soil before the greenhouse，straw returning and adding microbial agents significantly increased the electrical conductivity，and the contents of soil water-soluble organic carbon，nitrate nitrogen，ammonium nitrogen and available potassium increased by 58.25%，219.50%，34.59% and 101.70%，respectively.Straw returning changed the composition of soil fungal community.Compared with no straw returning，straw returning increased the relative abundance of Ascomycota by 9.17% and decreased the relative abundance of Mortierella by 0.17%.However，the relative abundance of the dominant genus Mortierella was significantly reduced by 8.29% compared with that before the greenhouse.The order of influence of soil physical and chemical factors on fungal community diversity in all samples was nitrate nitrogen>ammonium nitrogen>available phosphorus>EC value>pH>water-soluble organic carbon>available potassium.Straw returning of greenhouse vegetables significantly increased the contents of water-soluble organic carbon，nitrate nitrogen，ammonium nitrogen and available potassium in soil，and the increase was more significant in the treatment of straw returning with decomposition agent.The in-situ returning of vegetable straw changed the composition of fungal community，but did not increase the content of pathogenic bacteria，and there were no pests and diseases in the process of plant growth，indicating that the combination of in-situ returning of straw and greenhouse was a scientific agronomic measure.

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备注/Memo

第一作者简介：何义利（1997-），男，硕士研究生，研究方向为农业资源利用。E-mail：1165542483@qq.com.责任作者：魏丹（1965-），女，博士，研究员，现主要从事土壤改良和废弃物循环利用等研究工作。E-mail:wd2087@163.com.基金项目：国家重点研发计划资助项目（2022YFD2002103）；北京市农林科学院青年科研基金资助项目(QNJJ202132)；北京市农林科学院科技创新能力建设专项资助项目（KJCX20230219，KJCX20230422）；北京市农林科学院植物营养与资源环境研究所团队科技能力提升资助项目（ZHS202304）。收稿日期：2023-04-27

更新日期/Last Update: 2023-12-20