[1]赵越,孙宇峰,徐磊,等.KASP标记技术在作物基因定位中的应用进展[J].北方园艺,2023,(19):122-127.[doi:10.11937/bfyy.20231080]
　ZHAO Yue,SUN Yufeng,XU Lei,et al.Application Progress of KASP Marker Technology on Crop Gene Location[J].Northern Horticulture,2023,(19):122-127.[doi:10.11937/bfyy.20231080]
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KASP标记技术在作物基因定位中的应用进展

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年19 页码: 122-127 栏目: 出版日期: 2023-10-15
Title:
Application Progress of KASP Marker Technology on Crop Gene Location
文章编号:
1001-0009(2023)19-0122-06
作者:
赵越1; 孙宇峰1; 徐磊2; 王盼1; 边境1; 王晓楠1
（1.黑龙江省科学院 大庆分院，黑龙江 大庆 163319;2.黑龙江省农业科学院 大庆分院，黑龙江 大庆 163319）
Author(s):
ZHAO Yue1; SUN Yufeng1; XU Lei2; WANG Pan1; BIAN Jing1; WANG Xiaonan1
(1.Daqing Branch,Heilongjiang Academy of Sciences，Daqing，Heilongjiang 163319;2.Daqing Branch,Heilongjiang Academy of Agricultural Sciences，Daqing，Heilongjiang 163319)
关键词:
单核苷酸多态性; 竞争性等位基因特异性聚合酶链式反应; 作物性状; 遗传图谱; 基因定位
Keywords:
single nucleotide polymorphism (SNP); kompetitive allele specific PCR (KASP); crop traits; genetic map; gene location
分类号:
S 188
DOI:
10.11937/bfyy.20231080
文献标志码:
A
摘要:
单核苷酸多态性（single nucleotide polymorphism，SNP）是基于下一代测序（next generation sequencing，NGS）发展起来的第三代分子标记，单个核苷酸的变异导致核酸序列出现多态性，具有遗传稳定性好、密度高和二等位基因型等特点，容易实现高通量和自动化检测。竞争性等位基因特异性聚合酶链式反应（kompetitive allele specific PCR，KASP）是一种基于SNP的无凝胶荧光聚合酶链式反应基因分型技术，已广泛应用于动植物遗传育种研究中。该研究从KASP技术在作物基因定位研究中的试验群体选择及具体应用策略2个方面，阐述了该技术在作物产量、品质和抗性等重要性状相关基因精细定位中的研究进展，并总结了其优缺点，提出了优化作物KASP分型技术体系和建立功能基因多态性位点KASP数据库等建议，进一步推广其应用，旨在为作物性状遗传调控研究提供参考依据，加快作物从传统的经验育种到精准育种转化。
Abstract:
Based on next generation sequencing (NGS)，single nucleotide polymorphism (SNP) of the third-generation molecular marker was developed.Mutations of a single nucleotide cause polymorphism in nucleic acid sequences.It has the characteristics of good genetic stability，high density and secondary genotype，and is easy to realize high throughput and automatic detection.Kompetitive allele specific PCR (KASP) is a genotyping technology which is a gel-free fluorescent polymerase chain reaction based on SNP and has been widely used in genetic breeding of animals and plants.In this study，the research progresses of KASP technology in fine mapping of genes related to important traits such as crop yield，quality and resistance were reviewed from experimental population selection and specific application strategies in crop gene location.And its advantages and disadvantages were summarized.Suggestions such as crop KASP typing technology system optimization and KASP database of functional gene polymorphic loci established were put forward to further promote its application,in order to provide reference for the genetic regulation of crop traits and accelerate the transformation from traditional experience breeding to precision breeding.

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备注/Memo

第一作者简介：赵越（1985-），女，硕士，助理研究员，现主要从事麻类遗传育种等研究工作。E-mail:15245990291@163.com.责任作者：王晓楠（1981-），女，博士，研究员，现主要从事麻类育种及分子生物学等研究工作。E-mail:wxn-fern@163.com.基金项目：黑龙江省科学院科学研究基金资助项目（KY2023DQ01）；大庆市指导性科技计划资助项目（zd-2021-96）；黑龙江省属科研院所科研业务费重点资助项目（CZKYF2021-2-B011）；中央引导地方科技发展专项资助项目（ZY20B09）；黑龙江省省属科研院所科研业务费资助项目（KYYW2022DQ01）。收稿日期：2023-03-31

更新日期/Last Update: 2023-11-29