SUN Yangyang,WANG Xiaoying,DONG Lijuan,et al.Genetic Diversity Analysis of Natural Hybrid F1 Generation of Red-kernel Walnut Based on SRAP Molecular Labeling[J].Northern Horticulture,2023,(20):28-33.[doi:10.11937/bfyy.20230641]
基于SRAP分子标记红仁核桃自然杂交F1代的遗传多样性分析
- Title:
- Genetic Diversity Analysis of Natural Hybrid F1 Generation of Red-kernel Walnut Based on SRAP Molecular Labeling
- 文章编号:
- 1001-0009(2023)20-0028-06
- Keywords:
- red-kernel walnut; hybrid F1 generation; SRAP; genetic diversity
- 分类号:
- S 664.1
- 文献标志码:
- A
- 摘要:
- 以“希尔”、“香玲”、红仁核桃‘Robert Livermore’及7个‘Robert Livermore’自然杂交F1代优株共10个核桃品种（优株）为试材，采用SRAP分子标记技术进行了遗传多样性分析，以期揭示红仁核桃自然杂交F1代的遗传多样性，为红仁核桃新品种选育提供参考依据。结果表明：筛选得到的25对引物共扩增出120条带，多态性比率为80%，样品间的平均Nei′s遗传多样性指数为0.271 0，平均Shannon信息指数为0.406 5，说明10个核桃品种（优株）间的遗传多样性较为丰富、遗传差异性较大；10个核桃品种（优株）遗传相似系数变化范围为0.591 7~0.808 3，在遗传相似系数阂值0.704处，可将10个核桃品种（优株）划分为3类，其中“香玲”单独聚为一类，与其它9个品种（优株）亲缘关系较远，‘Robert Livermore’实生F1代中种皮颜色红色的聚为一类，种皮颜色黄色的聚为一类。
- Abstract:
- Taking ‘Hill’‘Xiangling’‘Robert Livermore’ and 7 ‘Robert Livermore’ natural hybrid F1 superior plants as experimental materials, a total of 10 walnut varieties (superior plants) were analyzed for genetic diversity using SRAP molecular marker technology, in order to reveal the genetic diversity of the F1 generation of natural hybrid red walnut and provide reference for the breeding of new red walnut varieties.The results showed that a total of 120 bands were amplified from the 25 pairs of selected primers，and the polymorphism ratio was 80%.The average Nei′s genetic diversity index and Shannon information index were 0.271 0 and 0.406 5，indicating that the genetic diversity and genetic difference among the 10 walnut varieties (superior tree) were relatively rich.The genetic similarity coefficient of the 10 walnut varieties (superior tree) ranged from 0.591 7 to 0.808 3.Ten walnut varieties (superior tree) were divided into three categories when genetic similarity coefficient threshold of 0.704 were selected.‘Xiangling’ was classified into a separate class and had a distant relation with the other 9 walnut varieties (superior tree).Among the F1 generation of ‘Robert Livermore’，those with red seed coat color were clustered into one category，while those with yellow seed coat color were clustered into another category.
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备注/Memo
第一作者简介：孙阳阳（1991-），女，硕士，工程师，现主要从事经济林育种及栽培等研究工作。E-mail：sunyangyang91@163.com.责任作者：张继亮（1967-），男，本科，研究员，现主要从事经济林育种及栽培等研究工作。E-mail：zhangjiliang5518@163.com.基金项目：国家林业局“948”资助项目（2015-4-02）；泰安市科技创新发展资助项目（2021NS093）。收稿日期：2023-03-0