LI Bowen,JIA Yanan,ZHOU Qun,et al.Evaluation of Cold Resistance of Apple Branches With Different Rootstock and Spike Combinations[J].Northern Horticulture,2023,(19):36-43.[doi:10.11937/bfyy.20230457]
不同砧穗组合苹果枝条的抗寒性评价
- Title:
- Evaluation of Cold Resistance of Apple Branches With Different Rootstock and Spike Combinations
- 文章编号:
- 1001-0009(2023)19-0036-08
- Keywords:
- apple; rootstock spike combination; cold resistance
- 分类号:
- S 661.1
- 文献标志码:
- A
- 摘要:
- 以“青砧一号”/“秦脆”、“八棱海棠”/“秦脆”、‘M26’/“秦脆”脆砧穗组合的一年生深度休眠期苹果枝条为试材,分别在-15、-20、-25、-30、-35、-40 ℃的人工低温处理12 h,研究了相对电导率(REC),过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性,以及丙二醛(MDA)、游离脯氨酸(Pro)、可溶性蛋白质含量等生理指标的影响,以期为筛选抗寒性强的优良砧穗组合,为阿拉尔垦区嫁接苹果的抗寒栽培和引进砧穗组合提供参考依据。结果表明:随着温度的降低,3个砧穗组合苹果枝条的相对电导率逐渐升高,呈“S”形变化曲线;抗寒性强的砧穗组合随温度降低能保持较高的酶活性,丙二醛含量维持在较低水平,脯氨酸和可溶性蛋白质含量维持在较高水平。3个砧穗组合苹果品种在阿拉尔垦区的综合抗寒性表现为“青砧一号”/“秦脆”组合抗寒性强于“八棱海棠”/“秦脆”组合强于‘M26’/“秦脆”组合,半致死温度分别为-23.95、-21.35 ℃和-21.23 ℃。
- Abstract:
- The one-year-old deeply dormant apple shoots combined with ‘Green Anvil No.1’/‘Qin Crisp’,‘Begonia Octopus’/‘Qin Crisp’,‘M26’/‘Qin Crisp’ anvil were treated at artificial low temperature at -15,-20,-25,-30,-35 ℃ and -40 ℃ for 12 hours,respectively.The effects of low temperature on the relative conductivity (REC),catalase (CAT),peroxidase (POD) enzyme,superoxide dismutase (SOD) activity,malondialdehyde (MDA),free proline (Pro),soluble protein content and other physiological indexes were studied,in order to screen out excellent anvil combinations with strong cold resistance,and provide reference for the cold resistance cultivation and introduction of anvil combinations of grafted apples in the Aralken area.The results showed that with the decrease of temperature,the relative conductivity of apple branches of the three anvil combinations gradually increased,showed an ‘S’ shaped change curve.The combination of anvils with strong cold resistance maintained high enzyme activity with decreasing temperature,malondialdehyde content remained low,and proline and soluble protein content remained at high levels.The comprehensive cold resistance of the three anvil combination apple varieties in the Aralken area was as follows,the cold resistance of the ‘Anvil No1’/‘Qin Crisp’ combination was stronger than that of ‘Begonia’/‘Qin Crisp’ combination was stronger than that of ‘M26’/‘Qin Crisp’ combination,and the semi-lethal temperatures were -23.95,-21.35 ℃ and -21.23 ℃,respectively.
参考文献/References:
[1]吴佳锐.阿克苏冰糖心苹果区域品牌建设研究[D].武汉:武汉轻工大学,2019.[2]陈学森,毛志泉,王楠,等.红肉和红皮苹果助力产业振兴和健康中国建设[J].果农之友,2020(10):1-2.[3]杜学梅,杨廷桢,高敬东,等.苹果砧木对嫁接品种影响的研究进展[J].西北农业学报,2020,30(4):487-495.[4]李欣.10种紫斑牡丹在哈尔滨地区的抗寒性研究[D].哈尔滨:东北林业大学,2009.[5]丛日征,张吉利,王思瑶,等.植物抗寒性鉴定及其生理生态机制研究进展[J].温带林业研究,2020,3(1):27-33.[6]THOMAS L,NAOKI N,NAOKO Y.Park pruning prompts a competitive reversal of anexotic tree,Ligustrum lucidum,in urban forests of Japan[J].Springer US,2016,19(3):1103-1118.[7]陈禹兴,付连双,王晓楠,等.低温胁迫对冬小麦恢复生长后植株细胞膜透性和丙二醛含量的影响[J].东北农业大学学报,2010,41(10):10-16.[8]刘兴禄,王红平,孙文泰,等.5个砧木苹果枝条的抗寒性评价[J].果树学报,2021,38(8):1264-1274.[9]范宗民,孙军利,赵宝龙,等.低温胁迫下不同砧木赤霞珠葡萄枝条抗寒性比较[J].果树学报,2021,37(2):215-225.[10]王飞雪.不同苹果砧木实生后代抗寒性研究[D].塔里木:塔里木大学,2020.[11]吕优伟,贺佳园,白小明,等.9个野生早熟禾对低温胁迫的生理响应及苗期抗寒性评价[J].草地学报,2014,22(2):326-333.[12]于庆帆,王海琪,白茹,等.隶属函数法对伊犁地区‘树上干’杏不同株系抗寒性的评价[J].分子植物育种,2018,16(8):2671-2676.[13]玉苏莆·阿不力提莆,阿依古丽·铁木儿,帕提曼·阿布都热合曼,等.利用隶属函数法综合评价梨砧木抗寒性[J].中国农业大学学报,2014,19(3):121-129.[14]丁红映,王明,谢洁,等.植物低温胁迫响应及研究方法进展[J].江苏农业科学,2019,47(14):31-36.[15]马艺杰,苏世平,李毅,等.核桃不同品种间抗寒能力比较[J].果树学报,2018,35(8):987-996.[16]梁锁兴,席海源,王文平,等.电解质渗出率配合logistic方程鉴定7个平欧杂种榛品种(系)的抗寒性[J].农学学报,2017,7(4):34-38.[17]李瑞雪,金晓玲,胡希军,等.6种含笑属植物抗寒性分析与综合评价[J].生态学杂志,2017,28(5):1464-1472.[18]叶翠.温度变化对植物生长期的影响[J].广东蚕业,2020,54(10):7-8.[19]ALONEN P,BUSZARD D.Current state of cold hardiness research on fruit crops[J].Canadian Journal of Plant Science,1997,77(3):399-420.[20]徐继忠等,苹果矮化砧木选育与栽培技术研究[M].北京:中国农业出版社,2016.[21]李雪冬.不同苹果品种及杂交优系果实品质及抗逆性评价[D].塔里木:塔里木大学,2022.[22]郭彬,高水练,王苗苗,等.盆栽条件下茶园土壤 pH 值,养分和酶活性对茶树修剪枝与大豆交互作用的响应[J].生态学杂志,2021,40(11):3561.[23]范宗民,孙军利,赵宝龙,等.不同砧木 ‘赤霞珠’葡萄枝条抗寒性比较[J].果树学报,2020,37(2):215-225.[24]徐佼俊.花烛深绿突变体与野生型形态特征及耐冷性比较[D].南京:南京农业大学,2016.[25]毛云飞.苹果砧木优系12-2抗连作障碍鉴定及机理研究[D].泰安:山东农业大学,2022.[26]姚迎丽,李春友,彭伟秀,等.三种观枝干植物的抗寒性评价[J].湖北农业科学,2022,61(9):95.[27]撒俊逸,白茹,孙志红,等.自然越冬过程中库尔勒香梨抗寒性生理指标的变化[J].石河子大学学报(自然科学版),2016,34(2):170-175.[28]曹建东,陈佰鸿,王利军,等.葡萄抗寒性生理指标筛选及其评价[J].西北植物学报,2010,30(11):2232-2239.[29]陈玖红,王沛,王平,等.6份披碱草属牧草种质材料抗寒性的比较[J].草业科学,2019,36(6):1591-1599.[30]张天翔,曹明华,林艺华,等.应用电导法配合 Logistic 方程确定‘多福’甜椒的抗寒性[J].分子植物育种,2018,16(12):4068-4071.[31]王玮,李红旭,赵明新,等.7个梨品种的低温半致死温度及耐寒性评价[J].果树学报,2015,32(5):860-865.[32]欧欢,王绪春,王振磊,等.自然越冬过程中扁桃枝条生理特性变化与抗寒性评价[J].新疆农业科学,2017,54(10):1785-1795.[33]孙欧文.高温干旱胁迫对4个绣球品种生理生化特性的影响[D].杭州:浙江农林大学,2019.[34]杨盛,郭黄萍,郝国伟,等.山西部分梨品种抗寒性测定及生理指标研究[J].农学学报,2014,4(12):72-77.[35]吉春容,刘海蓉,罗继,等.低温冻害过程中枣树枝条相关生理指标的变化[J].中国农业气象,2016,37(5):545-554.[36]陈新华,郭婧,祁雷,等.低温胁迫对甜樱桃一年生枝条的影响[J].果树学报,2014,31(S1):124-128.[37]王依,靳娟,罗强勇,等.4个酿酒葡萄品种抗寒性的比较[J].果树学报,2015,32(4):612-619.
相似文献/References:
[1]曹慧,姜倩倩,张保仁,等.节水养根处理对苹果枝叶生长发育的影响[J].北方园艺,2013,37(04):1.
[2]王贵平,王金政,薛晓敏,等.叶面喷施甜菜碱对干旱胁迫下苹果幼树[J].北方园艺,2014,38(12):10.
WANG Gui-ping,WANG Jin-zheng,XUE Xiao-min,et al.Effect of Spraying Glycinebetaine on Physiological Responses of Apple Young Trees Under Drought Stress[J].Northern Horticulture,2014,38(19):10.
[3]李 莹,任亚梅,马学敏,等.1-MCP处理结合不同贮藏条件对苹果常温货架品质的影响[J].北方园艺,2014,38(11):137.
LI Ying,REN Ya-mei,MA Xue-min,et al.Effects of 1-MCP Treatment With Different Storage Conditions on Quality of Apple During Shelf Life at Room Temperature[J].Northern Horticulture,2014,38(19):137.
[4]党建美,胡清玉,张 瑜,等.炭疽菌叶枯病在我国苹果产区的发生分布及趋势分析[J].北方园艺,2014,38(10):177.
DANG Jian-mei,HU Qing-yu,ZHANG Yu,et al.The Occurrence Trend and Distribution of Apple Glomerella Leaf Spot in China[J].Northern Horticulture,2014,38(19):177.
[5]解贝贝,沙广利,戴洪义,等.六种无融合生殖砧木对‘嘎拉’苹果果实大小和品质的影响[J].北方园艺,2013,37(17):23.
XIE Bei-bei,SHA Guang-li,DAI Hong-yi,et al.Effect of Six No Fusion Reproductive Rootstocks on the Fruit Size and Quality of ‘Gala’ Apple[J].Northern Horticulture,2013,37(19):23.
[6]冯娟娟,刘军弟,霍学喜.世界主要落叶水果主产国生产与贸易预测分析[J].北方园艺,2014,38(04):195.
[7]吕天星,王铭,伊凯.苹果杂种实生苗叶片性状遗传趋势研究[J].北方园艺,2013,37(14):8.
LV Tian-xing,WANG Ming,YI Kai.Study on Genetic Trends of Leaf Traits in Apple Hybrid Seedling[J].Northern Horticulture,2013,37(19):8.
[8]史星雲,王立新,王亚杰,等.陕西省苹果主栽品种品质差异及相关性分析[J].北方园艺,2013,37(14):11.
SHI Xing-yun,WANG Li-xin,WANG Ya-jie,et al.Quality and Correlation Analysis of the Main Apple Cultivars in Shaanxi Province[J].Northern Horticulture,2013,37(19):11.
[9]隗晓雯,郭静,王菲,等.不同矮化砧木叶片酶活性与内源激素含量的差异[J].北方园艺,2013,37(14):16.
WEI Xiao-wen,GUO Jing,WANG Fei,et al.Difference of Enzyme Activity and the Content of Endogenous Hormones in the Leaves of Different Apple Dwarfing Rootstocks[J].Northern Horticulture,2013,37(19):16.
[10]刘 君,刁永强,陈淑英,等.伊犁河谷不同苹果品种苗木冻害调查及分析[J].北方园艺,2013,37(23):34.
LIU Jun,DIAO Yong-qiang,CHEN Shu-ying,et al.An Investigation and Analysis of Freeze Injury of Different Varieties Apple Seedlings in Ili Area[J].Northern Horticulture,2013,37(19):34.
[11]霍强强,李高潮,曹珊,等.不同砧穗组合对苹果生长、品质及产量的影响[J].北方园艺,2017,41(10):25.[doi:10.11937/bfyy.201710006]
HUO Qiangqiang,LI Gaochao,CAO Shan,et al.Effects of Different Stock-scion Combinations on Apple Growth, Fruit Quality and Yield[J].Northern Horticulture,2017,41(19):25.[doi:10.11937/bfyy.201710006]
[12]党艳青,姜喜,焦灰敏,等.苹果不同矮化砧穗组合的成活率及生长调查[J].北方园艺,2018,42(13):39.[doi:10.11937/bfyy.20174627]
DANG Yanqing,JIANG Xi,JIAO Huimin,et al.Investigation of Survival Rate and Growth Indices of Different Dwarf Apple Scion-stock Combinations[J].Northern Horticulture,2018,42(19):39.[doi:10.11937/bfyy.20174627]
备注/Memo
第一作者简介:栗博文(1999-),女,硕士研究生,研究方向为农艺与种业。Email:2147344883@qq.com.责任作者:王江波(1979-),女,博士,副教授,硕士生导师,现主要从事果树育种与生物技术等研究工作。E-mail:wjbok@126.com.基金项目:新疆生产建设兵团科技合作计划资助项目(2020BC005);兵团南疆重点产业创新发展支撑计划资助项目(2017BD007)。收稿日期:2022-02-17