QU Shixu,SUO Yizhen,YUAN Haipeng,et al.Effects of Salt Stress on Physiological Characteristics and Secondary Metabolites of Cannabis sativa[J].Northern Horticulture,2023,(19):106-113.[doi:10.11937/bfyy.20230390]
盐胁迫对火麻生理特性及次生代谢产物的影响
- Title:
- Effects of Salt Stress on Physiological Characteristics and Secondary Metabolites of Cannabis sativa
- 文章编号:
- 1001-0009(2023)19-0106-08
- Keywords:
- Cannabis sativa L.; salt stress; physiological characteristics; total flavonoids; cannabidiol
- 分类号:
- S 567.21+9
- 文献标志码:
- A
- 摘要:
- 以火麻（Cannabis sativa L.）为试材，采用正常生长的火麻幼苗为对照，用不同浓度（100、200、300、400 mmol·L-1）盐溶液胁迫处理火麻幼苗，观测各生理指标和总黄酮、大麻二酚等次生代谢产物含量变化，研究了在盐（NaCl）胁迫下火麻的生理生化特性及其次生代谢产物含量的影响，以期为火麻的栽培和耐盐适应性提供参考依据。结果表明：随着盐浓度的升高和胁迫时间的延长，火麻净光合速率、气孔导度、蒸腾速率、最大光化学效率（Fv/Fm）和光合色素均呈现持续降低趋势；而丙二醛含量和脯氨酸含量则随着盐浓度升高呈现逐渐增加的趋势（400 mmol·L-1下的脯氨酸除外），可溶性蛋白质、可溶性糖、过氧化氢酶（CAT）、过氧化物酶（POD）、抗坏血酸过氧化氢酶（APX）、超氧化物歧化酶（SOD）活性和总黄酮含量均随盐浓度的增大呈现先升高后降低的趋势，并在200 mmol·L-1盐浓度下时达到最大值；在100 mmol·L-1盐浓度下处理1~5 d时大麻二酚含量较CK略有升高，但差异不显著（P>0.05），而在200~400 mmol·L-1处理下，随盐浓度升高和处理时间的延长大麻二酚含量均持续降低，但与对照相比具有显著差异（P<0.05）。由此说明，火麻对盐胁迫有一定的耐受性，在200 mmol·L-1以下浓度的盐胁迫下，火麻能够维持基本的生理活动，盐浓度高于200 mmol·L-1火麻生理受到抑制，并与盐浓度呈现正相关。
- Abstract:
- Taking Cannabis sativa L.as the test material，the seedlings were treated with salt solution of different concentrations (100，200，300，400 mmol·L-1)，normal growing seedlings were used as control (CK)，the changes of physiological indexes，and contents of secondary metabolites such as total flavonoids and cannabidiol were observed.The physiological and biochemical characteristics of C.sativa under salt (NaCl) stress and the effects of secondary metabolites content were studied，in order to provide reference for cultivation and salt-tolerance adaptability of C.sativa.The results showed that the net photosynthetic rate，stomatal conductance，transpiration rate，maximum photochemical efficiency (Fv/Fm) and photosynthetic pigment decreased continuously with the increase of salt concentration and the prolongation of stress time.The contents of malondialdehyde and proline increased gradually with the increase of salt concentration (except proline under N4 treatment).The activities of soluble protein，soluble sugar，catalase (CAT)，peroxidase (POD)，ascorbate catalase (APX)，superoxide dismutase (SOD) and total flavonoid content increased first and then decreased with the increase of salt concentration，and reached the maximum value at 200 mmol·L-1 salt concentration.When treated at 100 mmol·L-1 salt concentration for 1 to 5 days，CBD content was slightly higher than that of CK，but the difference was not significant (P>0.05)，while under 200-400 mmol·L-1 treatment，CBD content was continuously decreased with the increase of salt concentration and the extension of treatment time，but there was a significant difference compared with CK (P<0.05).These results indicated that C.sativa had a certain tolerance to salt stress.Under salt stress below 200 mmol·L-1，C.sativa could maintain basic physiological activities，while salt concentration above 200 mmol·L-1，C.sativa physiology was inhibited and positively correlated with salt concentration.
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备注/Memo
第一作者简介：屈施旭（1996-），女，硕士研究生，研究方向为植物生理生态学。E-mail：996717353@qq.com.责任作者：张玉红（1972-），男，博士，教授，现主要从事植物胁迫生理学等研究工作。E-mail：pzhangyh@126.com.基金项目：森林植物生态学教育部重点实验室（东北林业大学）开放基金资助项目（KF2022013）。收稿日期：2023-02-08