[1]王帆帆,毛 婷,唐 涛,等.湖北贝母灰霉病病原菌鉴定及防治药剂筛选[J].北方园艺,2023,(17):102-109.[doi:10.11937/bfyy.20230276]
　WANG Fanfan,MAO Ting,TANG Tao,et al.Identification and Screening Fungicide of the Pathogen of Gray Mold on Fritillaria hupehensis Hsiao[J].Northern Horticulture,2023,(17):102-109.[doi:10.11937/bfyy.20230276]
点击复制

湖北贝母灰霉病病原菌鉴定及防治药剂筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年17 页码: 102-109 栏目: 出版日期: 2023-09-15
Title:
Identification and Screening Fungicide of the Pathogen of Gray Mold on Fritillaria hupehensis Hsiao
文章编号:
1001-0009(2023)17-0102-08
作者:
王帆帆1; 毛 婷1; 唐 涛1; 2; 段媛媛1; 郭晓亮1; 2; 游景茂1
（1.湖北省农业科学院 中药材研究所，农业农村部中药材生物学与栽培重点实验室，湖北省中药材规范化生产（GAP）工程研究中心，湖北 恩施 445000；2.湖北省农业科学院 林下经济湖北省工程研究中心，湖北 武汉 430064）
Author(s):
WANG Fanfan1; MAO Ting1; TANG Tao1; 2; DUAN Yuanyuan1; GUO Xiaoliang1; 2; YOU Jingmao1
(1.Institute of Chinese Herbel Medicine,Hubei Academy of Agricultural Sciences/Key Laboratory for Biology and Cultivation of Chinese Medicinal Materials of Ministry of Agriculture and Rural Affairs,Hubei Standardized Production of Traditional Chinese Medicine (GAP) Engineering Research Center,Enshi,Hubei 445000；2.Hubei Provincial Engineering Research Center of Under-forest Economy,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064)
关键词:
湖北贝母; 葡萄孢属; 分子生物学; 生物学特性; 杀菌剂
Keywords:
Fritillaria hupehensis Hsiao; Botrytis sp.; molecular biology; biological characteristics; fungicide
分类号:
S 567.9
DOI:
10.11937/bfyy.20230276
文献标志码:
A
摘要:
以湖北贝母病样为试材，采用组织分离法和菌丝生长速率法，明确了湖北贝母灰霉病病原菌的种类，研究了不同杀菌剂对该病原菌的影响，以期为病害的科学防控提供参考依据。结果表明：湖北贝母灰霉病的致病菌属葡萄孢属（Botrytis sp.），是一种尚未报道过的新种。病原菌BMXTX2菌丝生长的最适pH为4，最适温度为20 ℃，最适碳氮源分别为葡萄糖和牛肉浸膏。室内毒力测定结果表明，430 g·L-1戊唑醇悬浮剂、8%氟硅唑微乳剂和500 g·L-1氟啶胺悬浮剂3种化学杀菌剂的抑菌效果较好，EC50值分别为0.15、0.48、0.98 mg·L-1；1%蛇床子素水乳剂和80%乙蒜素乳油2种生物杀菌剂对病原菌的抑菌效果较好，EC50值均小于10 mg·L-1，也可作为备选药剂。该研究首次明确了湖北贝母灰霉病的病原菌，并筛选出5种有较好抑菌效果的杀菌剂，为湖北贝母灰霉病的田间防控提供了参考依据。
Abstract:
Taking the Fritillaria hupehensis Hsiao disease samples as the specimen,and tissue separation method and mycelium growth rate method were used,the pathogen species of F.hupehensis Hsiao was identified and the effects of different fungicides on the pathogen was studied,in order to provide reference for the effective prevention and control of gray mold on F.hupehensis Hsiao.The results showed that the pathogen of gray mold on F.hupehensis Hsiao belongs to Botrytis sp.,which was a new species.The optimum environment for mycelial growth of strain BMXTX2 was pH 4 and 20 ℃.Glucose and beef extract were the best carbon and nitrogen sources respectively.The results of toxicity measurement showed that 430 g·L-1tebuconazole SC,8% flusilazole ME and 500 g·L-1 fluazinam SC had strong inhibitory effect on the pathogen in the chemical fungicide,with EC50values of 0.15 mg·L-1,0.48 mg·L-1 and 0.98 mg·L-1,respectively.Among the biological fungicide,1% osthole EW and 80% ethylicin EC had a good antibacterial effect,with EC50<10 mg·L-1,which could be used as candidate agents.To our knowledge,this was the first report of Botrytis sp.causing gray mold on F.hupehensis Hsiao,and the five kinds of fungicides screened in this study will provide reference for the effective prevention and control of gray mold on F.hupehensis Hsiao.

参考文献/References:

［1］ 国家药典委员会.中华人民共和国药典，一部［M］.北京:中国医药科技出版处,2020.［2］ 段媛媛,游景茂,周武先,等.两种不同种植模式下湖北贝母生长调查及性状构成分析［J］.中药材,2019,42(1):13-17.［3］ 谭学超.湖北贝母的常见病害及其综合防治措施研究［J］.南方农业,2018,12(32):7-8.［4］ 王爽,李新民,刘春来,等.平贝母鳞茎腐烂病病原菌鉴定和药剂筛选［J］.植物保护,2020,46(6):65-70.［5］ 曹瑱艳.浙产中药材主要真菌病害分子诊断方法的建立及生防制剂的筛选［D］.杭州:中国计量大学,2020.［6］ 李吉二,温思思,张羽加,等.浙贝母灰霉病病原真菌的分子鉴定［J］.植物保护,2022,48(2):151-156.［7］ FUJINAWA M F,PONTES N,BOREL F C,et al.Biological control of gray mold and Myrothecium leaf spot in begonias［J］.Crop Prot,2020,133(7):105138.［8］ WHITE T J,BRUNS T,LEE S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.PCR protocols:A guide to methods and applications［J］.Academic Press,San Diego,1990,64(1):315-322.［9］ STAATS M,BAARLEN P V,SCHOUTEN A,et al.Positive selection in phytotoxic protein-encoding genes of Botrytis species［J］.Fungal Genet Biol,2007,44(1):52-63.［10］MARTIJN S,PETER V B,VAN K J A L.Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity［J］.Mol Biol Evol,2005,22(2):333-346.［11］GRANT-DOWNTON R T,TERHEM R B,KAPRALOV M V,et al.A novel Botrytis species is associated with a newly emergent foliar disease in cultivated Hemerocallis［J］.PLoS One,2014,9(6):e89272.［12］ANDREA R,MARILINDA L,GLACOMO Z,et al.Botrytis euroamericana,a new species from peony and grape in North America and Europe［J］.Mycologia,2017,109(3):495-507.［13］朱洪庆,王盼盼,张萌,等.rDNA-ITS序列分析法与传统分类法相结合在真菌鉴定中的应用［J］.西华师范大学学报(自然科学版),2016,37(3):264-269.［14］ERPER I,DELIGOZ I,OZER G,et al.First report of botrytis blight of tulip caused by Botrytis tulipae in Turkey［J］.J Plant Pathol,2018,101(2):427.［15］HE S Q,WEN Z H,BAI B,et al.Botrytis polygoni,a new species of the genus Botrytis infecting Polygonaceae in Gansu,China［J］.Mycologia,2021,113(1):78-91.［16］袁月,周如军,傅俊范,等.不同人参灰霉病的灰葡萄孢菌生物学特性及致病性比较［J］.沈阳农业大学学报,2016,47(3):271-277.［17］高智谋,李艳梅,李喜玲,等.源自不同寄主的灰葡萄孢生物学特性的比较研究［J］.菌物学报,2009,28(3):370-377.［18］殷辉,陈亚蕾,秦楠,等.藜麦灰霉病病原菌鉴定及环境因子对其菌丝生长和分生孢子萌发的影响［J］.植物保护学报,2022,49(3):899-908.［19］李聪丽,阳亚婕,傅本重,等.香葱灰霉病病原鉴定及其生物学特性研究［J］.河南农业科学,2017,46(9):73-78.［20］杜艳丽,曹兴,王桂清,等.百合灰霉病病原菌鉴定及其部分生物学特性测定［J］.南方农业学报,2019,50(2):307-314.［21］任亚峰,韦唯,李冬雪,等.葡萄灰霉病病原菌鉴定及生物学特性［J］.应用与环境生物学报,2019,25(5):1139-1144.［22］姜莉莉,田中一久,孙瑞红,等.草莓灰霉病病原菌的分离鉴定及室内毒力测定［J］.山东农业科学,2021,53(8):102-106.［23］唐涛,王帆帆,段媛媛,等.13种生物源杀菌剂对华重楼灰霉病的防治［J］.农药,2021,60(4):297-300.［24］阮若昕,罗秀俊,王明爽,等.杀菌剂对浙贝母灰霉病菌室内毒力的测定［J］.浙江农业科学,2020,61(12):2603-2604.［25］陈乐,苗则彦,孙柏欣,等.灰霉病菌抗药性研究进展［J］.中国植保导刊,2020,40(4):21-30.

备注/Memo

第一作者简介：王帆帆（1994-），女，硕士，助理研究员，现主要从事中药材病害绿色防控等研究工作。E-mail:wff164229@163.com.责任作者：〖JP3〗游景茂（1983-），男，博士，副研究员，现主要从事中药材病虫害防控等研究工作。E-mail:jingmaoyou@126.com.基金项目：恩施州科技计划资助项目（XYJ2022000027）；中央引导地方科技发展资金资助项目（2022BGE256）；湖北省农业科学院青年科学基金资助项目（2023NKYJJ30）；湖北省农业科技创新中心重大科技研发资助项目（2020-620-000-002-04）；湖北省重点研发资助项目（2022BBA153）；现代农业产业技术体系建设专项资助项目（CARS-21）。收稿日期：2023-02-02

更新日期/Last Update: 2023-11-16