YU Xin,LIU Jiarong,LI Nan,et al.Biological Characteristics and Fungicide Sensitivity of Acremonium sclerotigenum of Longya Lilium brownii Soft Rot Pathogen[J].Northern Horticulture,2023,(15):115-121.[doi:10.11937/bfyy.20224847]
龙牙百合软腐病菌Acremonium sclerotigenum生物学特性及药剂敏感性
- Title:
- Biological Characteristics and Fungicide Sensitivity of Acremonium sclerotigenum of Longya Lilium brownii Soft Rot Pathogen
- Keywords:
- Lilium brownii var. viridulum; Acremonium sclerotigenum; biological characteristics; drug sensitivity
- 文献标志码:
- A
- 摘要:
- 以龙牙百合软腐病病原菌产核枝顶孢Acremonium sclerotigenum为试材，采用菌丝生长速率法和孢子计数法，研究不同温度、pH、光照、碳源和氮源等处理对病原菌菌丝生长和产孢的影响以及病原菌对5 种杀菌剂的敏感性，以期为田间龙牙百合软腐病的防治提供参考依据。结果表明：最适宜菌丝生长的条件包括温度 25 ℃，pH 7，12 h 光照(光强为112 lx)/12 h黑暗，碳源为可溶性淀粉，氮源为硝酸铵；最适宜产孢条件包括温度为30 ℃，pH 6，24 h 黑暗，碳源为果糖，氮源为牛肉浸膏。供试杀菌剂中化学与生物杀菌剂抑菌效果最好的分别是430 g·L-1戊唑醇和1%申嗪霉素，其EC50值分别为0.004 8 mg · L-1和3.587 2 mg · L-1。病原菌菌丝致死温度为62 ℃、分生孢子致死温度为63 ℃；对番茄、黄瓜等作物具有较强致病性。
- Abstract:
- Taking Acremonium sclerotigenum of Longya Lilium brownii soft rot as test material，mycelial growth and sporulation were studied under the effects of different temperatures，pH，light，carbon and nitrogen sources conditions by using the mycelial growth rate method and spore counting method and the sensitivity of the pathogen to 5 fungicides was determined by using the mycelial growth inhibition method，in order to provide reference for the prevention and treatment of Longya Lilium brownii soft rot pathogen in the field.The results showed that the optimum conditions for the mycelial growth of Acremonium sclerotigenum were found to be 25 ℃,pH 7,12 hours light (112 lx fluorescent lamp irradiation)/12 hours darkness，soluble starch for carbon,and ammonium nitrate for nitrogen.Optimal spores producing conditions 30 ℃，pH 6，24 hours dark，fructose for carbon source，beef extract for nitrogen source.Among the tested chemical and biocidal fungicides，430 g·L-1 tebuconazole or 1% phenazinE1carboxylicacid had the greatest inhibition with EC50 value of 0.004 8 mg·L-1and 3.587 2 mg·L-1， respectively.The lethal temperature of mycelia and conidia was 62 ℃ and 63 ℃，respectively.The pathogen had strong pathogenicity to tomato，cucumber and other crops.
参考文献/References:
[1]胡悦,杜运鹏,张梦,等.12种百合主要营养成分和活性成分的分析评价[J].天然产物研究与开发,2019,31(2)：292-298.[2]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2010.[3]李润根,曾巧灵,罗霞.龙牙百合生殖特性及繁育规律[J].北方园艺,2017(5)：93-96.[4]李润根,却志群,刘芳玲,等.万载龙牙百合花粉生活力测定及贮藏方法研究[J].安徽农业科学,2014,42(14):4404-4417.[5]李谋智.食用百合组织培养快繁技术研究[J].北方园艺,2006(1):101-102.[6]曾慧兰,刘佳蓉,李丽娜,等.龙牙百合软腐病的病原菌[J].菌物学报,2021,40(7):1639-1647.[7]郭云忠,孙广宇,高保卫,等.套袋苹果黑点病病原菌鉴定及其生物学特性研究[J].西北农业学报,2005,14(3):18-21.[8]王娟,胡军华,龙艳玲,等.交枝顶孢霉杀柑橘全爪螨活性及其生物学特性研究[J].植物保护,2017,43(2):95-101.[9]李培谦,药震,冯宝珍,等.克伦生葡萄内生菌Acremonium sclerotigenum的分离鉴定及致腐能力研究[J].食品与发酵工业，2022,48(15)：131-136.[10]王翠翠,王彩霞,金静,等.套袋苹果褐点病菌分类鉴定及致病性[J].菌物学报,2015,34(6)：1036-1045.[11]曲健禄,范昆,李晓军,等.多种化学药剂对套袋苹果黑点病菌的室内毒力及田间防治效果[J].江西农业学报,2008,20(11):65-68.[12]鲍竹.鸟巢蕨和珊瑚豆叶斑病的病原菌鉴定及生物学特性研究[D].大连:辽宁师范大学,2016.[13]菅盼盼.大连市几种草本花卉叶斑病病原菌的鉴定及其生物防治[D].大连:辽宁师范大学,2018.[14]翟浩,王金政,王丹,等.免套袋栽培苹果采后真菌病害鉴定及精油抑菌研究[J].果树学报,2021,38(4):549-559.[15]GHULE S B，SAWANT I S，SAWANT S D，et al.Isolation and identification of three new mycoparasites of Erysiphe necator for biological control of grapevine powdery mildew[J].Australasian Plant Pathology，2019,48(4)：351367.[16]LO P S，ALFONZO A，GIAMBRA S，et al.Identification of Acremonium isolates from grapevines and evaluation of their antagonism towards Plasmopara viticola[J].Annals of Microbiology，2015，65(4)：2393-2403.[17]姚秀英,刘南南,王桂清.菌核生枝顶孢霉次生代谢物对桃褐斑致病菌的抑制效果[J].农业科技与装备,2019,291(3):18-22.
相似文献/References:
[1]李润根,曾巧灵,罗霞.龙牙百合生殖特性及繁育规律[J].北方园艺,2017,41(05):93.[doi:10.11937/bfyy.201705022]
LI Rungen,ZENG Qiaoling,LUO Xia.Reproduction Characteristics and Rule of Lilium brownie var.viridulum[J].Northern Horticulture,2017,41(15):93.[doi:10.11937/bfyy.201705022]
[2]高柱基,王小玲,严毅,等.环境因子对龙牙百合脱毒组培苗移栽成活率的影响[J].北方园艺,2021,(06):66.[doi:10.11937/bfyy.20201331]
GAO Zhu,WANG Xiaoling,YAN Yi,et al.Effects of Environmental Factors on the Survival Rate of Tissue Culture Detoxified Bottle Seedlings of Lilium brownii var.viridulum[J].Northern Horticulture,2021,(15):66.[doi:10.11937/bfyy.20201331]
备注/Memo
第一作者简介：于鑫(1998-)，女，硕士研究生,研究方向为药物研发与生产技术。Email:yu15890729023@163.com.责任作者：李润根(1966-)，男，硕士，教授，现主要从事百合栽培及其连作障碍防治等研究工作。Email:13507058200@163.com.基金项目：国家自然科学基金资助项目（32160674）；江西省科技厅重点研发资助项目（20212BBG73039）；2020年国家级大学生创新创业训练计划资助项目（202010417012）。收稿日期：2022-11-27