WAN Cheng,XIONG Xue,LI Leijia,et al.Biological Characteristics and Preservation Technology of Introduced Agaricus blazei Murrill Mycelium[J].Northern Horticulture,2023,(11):113-122.[doi:10.11937/bfyy.20224251]
引种姬松茸菌丝体生物学特性及保藏技术
- Title:
- Biological Characteristics and Preservation Technology of Introduced Agaricus blazei Murrill Mycelium
- 文献标志码:
- A
- 摘要:
- 以引进省内外表现优良的11个姬松茸菌株为试材，采用基础生物学研究方法，研究了不同培养基、光照、温度以及pH对其菌丝生长的影响，同期进行了姬松茸菌种资源保藏技术研究，以期筛选出姬松茸保藏最适宜保护剂配方。结果表明：姬松茸菌株适合在含麦麸的培养基上生长，菌丝培养时适合在黑暗条件下，适宜温度范围在25~32 ℃，适宜酸碱环境在pH 6~7；姬松茸菌丝呈圆筒状，菌丝尖端排列分为放射形和卷曲形，其锁状联合现象较少、分布不密集；姬松茸菌种保藏时水溶液保护剂最佳配方为1∶1的25%甘油与5%蔗糖溶液。
- Abstract:
- Eleven strains of Agaricus blazei with excellent performance inside and outside the province were introduced.According to the basic biological research methods，the effects of different medium，light，temperature and pH on the growth of mycelium were studied.Meanwhile，the research on the preservation technology of Agaricus blazei Murrill strain resources was carried out，in order to screen out the most suitable protective agent formula for Agaricus blazei Murrill preservation.The results showed that Agaricus blazei Murrill was suitable for growth on the medium containing wheat bran，the hyphae were suitable for dark conditions，the suitable temperature range was 25-32 ℃，and the suitable acid-base environment was between pH 6-7.Agaricus blazei Murrill mycelium was cylindrical，and the tip of the mycelium was arranged into radial and curly shapes，with less clamp connection and less dense distribution.The optimal formula of aqueous solution protective agent for Agaricus blazei preservation was 25% glycerol and 5% sucrose solution in a ratio of 1∶1.
参考文献/References:
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备注/Memo
第一作者简介：万诚（1990-），男，硕士，助理研究员，现主要从事遗传育种等研究工作。E-mail：348590242@qq.com.责任作者：王莹（1982-），女，博士，副研究员，现主要从事生物学等研究工作。E-mail：66267469@qq.com.基金项目：贵州省科技重大专项资助项目（黔科合大专项字[2019]3008-3）；贵州省科技支撑资助项目（黔科合支撑[2019]2451号-8-11）。收稿日期：2022-10-19