[1]张伟,陈毓蔚,牛亚菲,等.红花草莓‘托斯卡纳’盐胁迫下的生长发育及生理响应[J].北方园艺,2023,(13):28-35.[doi:10.11937/bfyy.20223805]
　ZHANG Wei,CHEN Yuwei,NIU Yafei,et al.Growth and Physiological Responses of Red-flowered Strawberry ‘Toscana’ Under Salt Stress[J].Northern Horticulture,2023,(13):28-35.[doi:10.11937/bfyy.20223805]
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红花草莓‘托斯卡纳’盐胁迫下的生长发育及生理响应

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年13 页码: 28-35 栏目: 出版日期: 2023-07-15
Title:
Growth and Physiological Responses of Red-flowered Strawberry ‘Toscana’ Under Salt Stress
作者:
张伟; 陈毓蔚; 牛亚菲; 冯悦; 侯非凡; 亢秀萍
（山西农业大学 园艺学院，山西 太谷 030801）
Author(s):
ZHANG Wei; CHEN Yuwei; NIU Yafei; FENG Yue; HOU Feifan; KANG Xiuping
(College of Horticulture，Shanxi Agricultural University，Taigu,Shanxi 030801)
关键词:
红花草莓; 盐胁迫; 盐害等级; 生长发育; 生理指标
Keywords:
red flowered strawberry; salt stress; salt damage grade; growth; physiological indicators
DOI:
10.11937/bfyy.20223805
文献标志码:
A
摘要:
以红花草莓品种‘托斯卡纳’匍匐茎苗为试材，采用盆栽法研究盐胁迫对草莓生长状况及生理特性的影响，测定3个NaCl浓度处理后第7、14、21天和第28天的株高、根长、叶面积及耐盐性指数等生长指标，确定其盐害级别，同时测定电导率（REC）、丙二醛（MDA）、可溶性糖（SS）、可溶性蛋白质（SP）、脯氨酸（Pro）含量、超氧化物歧化酶（SOD）、过氧化物酶（POD）活性7个生理指标，研究盐害对‘托斯卡纳’植株生长、质膜透性、渗透调节物质、抗氧化酶活性的影响，以期探究红花草莓对盐胁迫的生长发育和生理响应，并为红花草莓生理响应盐胁迫提供参考依据。结果表明：红花草莓‘托斯卡纳’叶片的盐害症状随NaCl浓度的升高而越显著。100 mmol?L-1NaCl处理下，14 d时叶片出现轻度盐害症状，盐害等级Ⅰ级，表现为叶片边缘呈萎蔫状，但未失绿，植株生长正常；200、400 mmol?L-1 NaCl处理下，叶片在第14天时开始出现中度盐害症状，盐害等级达Ⅱ级，株高、叶面积的生长均受到显著抑制；盐处理显著引起了红花草莓的生理变化，‘托斯卡纳’的REC前1周内剧烈上升；各盐胁迫浓度下MDA含量差异显著，也呈上升趋势；叶片SS、SP、Pro含量与SOD、POD活性均随胁迫时间延长呈先上升后下降的趋势。相关性分析表明，REC与MDA含量、SOD活性呈显著正相关，与植株生长指标呈显著负相关；Pro含量与SS、SP及植株生长指标均呈显著正相关，与REC和MDA含量呈显著负相关。
Abstract:
Taking the stolon plants of ‘Toscana’ strawberry as experimental material，treated with three concentrations of NaCl，at the 7th，14th，21th and 28th days after treatment，the salt damage level of red-flowered strawberry were observed，and the effects of different salt concentrations on the growth of strawberry，plasma membrane permeability，osmotic regulators and antioxidant enzymes were investigated，including the plant height，leaf area，electrical conductivity，malondialdehyde，proline，soluble protein，soluble sugar，superoxide dismutase，peroxidase,in order provide reference for the physiological response of red-flowered strawberry to high salt stress，which has a great significance for the innovative utilization of it.The results showed that the symptoms of salt damage on the ‘Toscana’ became significant with the increase of NaCl concentration.Under the treatment of 100 mmol?L-1，the leaves showed slightly salt damage symptoms at the 14th day with the salt damage gradeⅠ，which showed that the leaf edge was wilting，but no chlorosis，and the plants grew normally.Under 200 mmol?L-1 and 400 mmol?L-1 NaCl，the leaves began to show moderate salt damage symptoms at the 14th day with the salt damage gradeⅡ，and the growth of plant height and leaf area of ‘Toscana’ were significantly inhibited.Salt treatment significantly caused the internal physiological changes of redflowered strawberry，and the REC increased quickly in the one week.The content of MDA also showed an upward trend.The activities of SS，SP，Pro contents，SOD and POD activities increased firstly and then decreased with the extension of stress time.Correlation analysis showed that REC was positively correlated with MDA content and SOD activity，and negatively correlated with plant growth index.Proline content was positively correlated with SS，SP content and plant growth index，and negatively correlated with REC and MDA content.

参考文献/References:

[1]雷家军，薛莉，代汉萍，等.红花草莓新品种‘粉佳人’和‘俏佳人’[J].园艺学报，2015，34(3)：599-600.[2]XUE L，WANG J，ZHAO J，et al.Study on cyanidin metabolism in petals of pink flowered strawberry based on transcriptome sequencing and metabolite analysis[J].BMC Plant Biol，2019（10）：1-12.[3]薛莉，刘玉，吴雪，等.红花草莓粉佳人与俏佳人杂交后代花与果实性状调查分析[J].沈阳农业大学学报，2019，50(2)：197-202.[4]李青云.草莓对盐胁迫的反应及调控研究[D].保定：河北农业大学，2005.[5]张超博，李傲，崔梦杰，等.干旱胁迫下‘宁玉’草莓生长发育及相关基因表达特点[J].园艺学报，2017，44(12)：2351-2360.[6]TURHAN E，GULEN H，ERIS A.The activity of antioxidative enzymes in three strawberry cultivars related to salt stress tolerance[J].Acta Physiol Plant，2008，30(2)：201-208.[7]QASIM M，ASHRAF M，REHMAN S，et al.Salt induced changes in two canola cultivars differing in salt tolerance[J].Biol Plant，2003，46：629-632.[8]齐琪，马书荣，徐维东.盐胁迫对植物生长的影响及耐盐生理机制研究进展[J].分子植物育种，2020，18(8)：2741-2746.[9]PRADAS，INMACULADA，CUEVAS，et al.Effects of salinity and nitrogen supply on the quality and healthrelated compounds of strawberry fruits (Fragaria × ananassa cv.Primoris)[J].J Sci Food Agr，1995(14)：2924-2930.[10]SUN Y，NIU G，WALLACE R，et al.Relative salt tolerance of seven strawberry cultivars[J].Horticulturae，2015（1）：27-43.[11]董天宇，朱旭东，张克坤，等.温度和盐胁迫对草莓开花进程和相关基因表达的影响[J].西北植物学报，2019，39(4)：638-647.[12]李文娟.不同草莓品种耐盐性比较及盐碱地对其生长和品质的影响[D].兰州：甘肃农业大学，2018.[13]孙永平，邵和平，季国军，等.NaCl胁迫对草莓不同叶龄叶片光合及生理指标的影响[J].江西农业学报，2012，24(2)：38-40.[14]GHADERI，NASSER，HATAMI，et al.Change in antioxidant enzymes activity and some morphophysiological characteristics of strawberry under longterm salt stress[J].Physiol Mol Biol Plant，2018，24(5)：833-843.[15]EL GOUMI Y，FAKIRI M，LAMSAOURI O，et al.Salt stress effect on seed germination and some physiological traits in three Moroccan barley (Hordeum vulgare L.) cultivars[J].J Mater Environ Sci，2014（5）：625-632.[16]陈秀娟，陈卫平，糜林，等.南方草莓叶面积计算方法的研究[J].中国农学通报，2009，25(14)：190-193.[17]高俊凤.植物生理学实验指导[M].北京：高等教育出版社，2006.[18]李合生，孙群，赵世杰.植物生理生化实验原理与技术[M].北京：高等教育出版社，2000.[19]薛莉，雷家军，刘源.红花草莓育种研究进展[J].东北农业大学学报，2012，43(10)：172-176.[20]曹兴，张秀省.可替代草坪的优质耐寒地被植物红花草莓[J].北方园艺，2008(6)：166-167.[21]TURHAN E，ERI A.Changes of micronutrients，dry weight and chlorophyll contents in strawberry plants under salt stress conditions[J].Commun Soil Sci Plant Anal，2005（36）:1021-1028.[22]YAGHUBI K H，GHADERI N，VAFAEE Y，et al.Potassium silicate alleviates deleterious effects of salinity on two strawberry cultivars grown under soilless pot culture[J].Sci Hortic，2016，213：87-95.[23]姚玉涛，张国新，丁守鹏，等.盐胁迫对草莓苗期生长及氧化还原系统的影响[J].北方园艺，2021(17)：22-29.[24]颜志明，魏跃，贾思振，等.盐胁迫对草莓抗氧化系统和离子吸收的影响[J].北方园艺，2013(9)：1-4.[25]FERREIRA J F S,XUAN L,SUAREZ D L.Fruit yield and survival of five commercial strawberry cultivars under field cultivation and salinity stress[J].Sci Hortic,2018,243:401-410.[26]GARRIGA M，MUNOZ C A，CALIGARI P D S，et al.Effect of salt stress on genotypes of commercial (Fragaria×ananassa) and Chilean strawberry (F.chiloensis)[J].Sci Hortic，2015，195：37-47.

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备注/Memo

第一作者简介：张伟（1990-），男，博士，讲师，现主要从事花卉种质资源与遗传育种等研究工作。Email：zwsxnd@sxau.edu.com.责任作者：亢秀萍（1962-），女，硕士，副教授，现主要从事野生花卉种质资源开发利用等研究工作。Email：sxndkxp@163.com.基金项目：山西省基础研究计划青年科学研究资助项目(SXBYKY2021079)；山西省高等学校科技创新资助项目(2021L117)。收稿日期：2022-09-15

更新日期/Last Update: 2023-08-18