MA Shuxiao,XI Suting,ZHU Zheng.Effects of Exogenous Salicylic Acid on Physiological Indexes in Rose Seedling Under Low Temperature Stress[J].Northern Horticulture,2023,(07):70-78.[doi:10.11937/bfyy.20223709]
外源水杨酸对低温胁迫月季苗生理指标的影响
- Title:
- Effects of Exogenous Salicylic Acid on Physiological Indexes in Rose Seedling Under Low Temperature Stress
- 文献标志码:
- A
- 摘要:
- 以月季品种“金凤凰”扦插苗为试材，采用生理指标测定和隶属函数分析方法，研究了不同浓度水杨酸(SA)对低温胁迫下月季生理指标的影响，以期为月季苗培育和生产上推广提供参考依据。结果表明：在低温胁迫下喷施SA可缓解月季苗叶片相对含水量和叶绿素的下降速度，抑制丙二醛(MDA)含量和相对电导率的过度增加，同时，有利于细胞渗透调节物质脯氨酸(Pro)、可溶性糖和可溶性蛋白质等含量的提高；尽管低温胁迫会诱导月季苗叶片内产生大量的过氧化氢(H2O2)，但外源SA能够通过提高超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性以及抗坏血酸(ASA)含量来抑制低温下H2O2的积累。综上所述，外施SA能够缓解低温下月季苗叶片的过氧化损伤，增强其对低温胁迫的缓冲作用，提高其耐低温能力，且通过隶属函数法分析显示，以喷施150 mg?L-1 SA的效果最佳。
- Abstract:
- Taking the rose cultivar ‘Jinfenghuang’ cuttings as test materials,physiological index determination and membership function analysis method were used,the effects of different concentrations of salicylic acid (SA) on the physiological indexes of rose under low temperature stress were studied,in order to provide reference for popularized and applied in the cultivation and production of rose seedlings.The results showed that spraying SA under low temperature stress could alleviate the decline rate of relative water content and chlorophyll in rose seedling leaves,inhibit the excessive increase of malondialdehyde (MDA) content and relative conductivity,meanwhile,it was beneficial to increase the content of cell osmotic regulator proline (Pro),soluble sugar and soluble protein；although low temperature stress could induce a large amount of hydrogen peroxide (H2O2) in the leaves of rose seedlings,exogenous SA could inhibit the accumulation of H2O2 by increase the activities of superoxide dismutase (SOD),peroxidase (POD) and catalase (CAT),and the content of ascorbic acid (ASA) at low temperature stress.In conclusion,external application of SA could alleviate the peroxidative damage of rose seedling leaves,and enhance its buffering effect under low temperature stress,and then improve its low temperature tolerance.In particular,it was analyzed by membership function method,the best effect of spraying was from 150 mg?L-1 SA.
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备注/Memo
第一作者简介：马曙晓（1981-），女，博士，副教授，现主要从事园林景观规划等研究工作。E-mail：864321735@qq.com.基金项目：邯郸市科学技术研究与发展计划资助项目（20312904012）。收稿日期：2022-09-09