[1]汤泳萍,盛文涛,周劲松,等.外源激素嘧啶醇与NAA对芦笋胚状体再生植株生长的影响[J].北方园艺,2023,(11):9-14.[doi:10.11937/bfyy.20223475]
　TANG Yongping,SHENG Wentao,ZHOU Jinsong,et al.Effects of Exogenous Hormones Pyrimidinol and NAA on the Growth of Regenerated Plants Derived From Garden[J].Northern Horticulture,2023,(11):9-14.[doi:10.11937/bfyy.20223475]
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外源激素嘧啶醇与NAA对芦笋胚状体再生植株生长的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年11 页码: 9-14 栏目: 出版日期: 2023-06-15
Title:
Effects of Exogenous Hormones Pyrimidinol and NAA on the Growth of Regenerated Plants Derived From Garden
作者:
汤泳萍1; 盛文涛2; 周劲松1; 尹玉玲1; 罗绍春1; 陈光宇1
（1.江西省农业科学院 蔬菜花卉研究所，江西 南昌 330200;2.南昌师范学院 生物技术研究院，江西 南昌 330032）
Author(s):
TANG Yongping1; SHENG Wentao2; ZHOU Jinsong1; YIN Yuling1; LUO Shaochun1; CHEN Guangyu1
(1.Institute of Vegetables and Flowers,Jiangxi Academy of Agricultural Sciences,Nanchang,Jiangxi 330200;2.Department of Biological Technology,Nanchang Normal University,Nanchang,Jiangxi 330032)
关键词:
芦笋; 嘧啶醇; NAA; 胚状体再生植株; 繁殖效率
Keywords:
garden asparagus; ancymidol; NAA; embryoid-derived plant; reproductive efficiency
DOI:
10.11937/bfyy.20223475
文献标志码:
A
摘要:
以芦笋花粉粒培养获得的胚状体为试材，在其形成的不定芽中，以MS+蔗糖30 g?L-1+琼脂粉8 g?L-1为基本培养基，添加不同质量浓度嘧啶醇（0、0.05、0.10、0.50、1.00、2.00 mg?L-1）和NAA（0、0.05、0.10、0.20、0.50、1.00 mg?L-1），研究了嘧啶醇和NAA浓度对不同基因型再生植株生长的影响，以期优化和提高芦笋植株体外扩繁效率。结果表明：培养基添加0.50 mg?L-1嘧啶醇处理的再生植株净生长高度、茎数分别增加1.05 cm和1.0根，根系变长，侧根增多；MS培养基添加0.50 mg?L-1嘧啶醇和0.20 mg?L-1 NAA后再生植株净生长高度、茎数分别增加1.60 cm和2.0根，根系生长旺盛；并且0.50 mg?L-1嘧啶醇和0.20 mg?L-1 NAA对芦笋胚状体再生植株的促进作用无基因型的显著差异。综上，在MS培养基中添加0.50 mg?L-1嘧啶醇和0.20 mg?L-1 NAA，可以提高芦笋胚状体再生植株繁殖效率。
Abstract:
The embryoids obtained from garden asparagus pollen grain culture were used as experimental materials.In the adventitious buds,MS+sucrose 30 g?L-1+agar powder 8 g?L-1 were used as the basic medium,pyrimidinol (0,0.05,0.10,0.50,1.00,2.00 mg?L-1) and naphthylacetic acid (NAA) (0,0.05,0.10,0.20,0.50,1.00 mg?L-1) with different mass concentrations were added,respectively.The effects of pyrimidinol and NAA concentrations on the growth of regenerated plants with different genotypes were studied,in order to optimize and improve the propagation efficiency of garden asparagus (Asparagus officinalis L.) plants in vitro.The results showed that the net growth height of regenerated plants treated with 0.50 mg?L-1 pyrimidinol in the medium increased by 1.05 cm and 1 root respectively,and the root system became longer and the lateral roots increased.After adding 0.50 mg?L-1 pyrimidinol and 0.20 mg?L-1 NAA to MS medium,the net growth height of regenerated plants,the number of stems increased by 1.60 cm and 2.0 roots,respectively,and the root system grew vigorously.And there was no significant difference among the genotypes in the promoting effect of 0.50 mg?L-1 pyrimidinol and 0.20 mg?L-1 NAA on plant regeneration from garden asparagus embryoids.It was speculated that adding 0.50 mg?L-1 pyrimidinol and 0.20 mg?L-1 NAA to MS medium could improve the reproductive efficiency of regenerated plants from garden asparagus embryoids.

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备注/Memo

第一作者简介：汤泳萍（1975-），女，硕士，研究员，现主要从事芦笋遗传育种等研究工作。E-mail：typ19751227@163.com.基金项目：江西省重点研发计划资助项目（20202BBFL63008）；井冈山红壤区芦笋产业关键技术研究与示范资助项目（JXXTCX201904-03）;江西省科技支撑重点资助项目（20141BBF60012）；江西省自然科学青年基金资助项目(20171BAB214024)；江西省教育厅科技资助项目（GJJ202619）。收稿日期：2022-08-24

更新日期/Last Update: 2023-07-25