GUAN Jinyan,TAN Jiana,CHEN Shuangyan,et al.Tissue Culture and Plant Regeneration of Euphorbia milii Ch.des Moulins[J].Northern Horticulture,2023,(08):56-62.[doi:10.11937/bfyy.20223382]
大花虎刺梅的组织培养和植株再生
- Title:
- Tissue Culture and Plant Regeneration of Euphorbia milii Ch.des Moulins
- Keywords:
- Euphorbia milii Ch.des Moulins; inflorescence involucre; tissue culture; plant regeneration
- 文献标志码:
- A
- 摘要:
- 以大花虎刺梅花朵为试材，采用组培快繁的方法，研究了取材部位、花朵的成熟程度、激素配比及浓度等因素对植株再生的影响，以期建立大花虎刺梅高效、适合工厂化生产的离体再生体系，为品种快繁和推广提供参考依据。结果表明：大花虎刺梅花序总苞上花芽经过生殖生长发育成二歧聚伞复状花序，组培培养使其经过营养生长诱导出再生植株。大花虎刺梅花朵的成熟度对出芽率影响比较明显，苞片半张开时的花序总苞是组培快繁的最佳取材部位。芽诱导的最佳培养基为MS+6-BA 1.0 mg?L-1+NAA 0.1 mg?L-1，出芽系数150.45%，芽体健壮，长势良好；丛芽增殖的最佳培养基为MS+6-BA 0.5 mg?L-1+NAA 0.2 mg?L-1，增殖系数4.03±0.43，植株健壮，叶片舒展墨绿，长势很好；生根培养的最佳培养基为MS+NAA 0.2 mg?L-1+IBA 0.4 mg?L-1，30 d后生根率达85%以上。移栽至消毒的珍珠岩∶泥炭土∶沙（1∶1∶1）的基质中定植，成活率85%。
- Abstract:
- The flowers of Euphorbia milii Ch.des Moulins were used as experimental materials,tissue culture and rapid propagation was adopted,the effects of plant parts,flower maturity,hormone ratio and concentration on plant regeneration were studied,in order to establish the high efficient regeneration system that suitable for factory production of Euphorbia milii Ch.des Moulins，provide reference for rapid propagation and popularization of variety.The results showed that the flower buds developed into a dichaploid cymose compound inflorescence during reproductive growth,and developed into regenerated plants through vegetative growth during tissue culture.The flower maturity had a great influence on the induction of plum buds of the Euphorbia milii Ch.des Moulins.Half-open flowers were the optimal part of the material for tissue culture.The optimal medium for shoot induction was MS+6-BA (1.0 mg?L-1)+NAA (0.1 mg?L-1).The budding coefficient was 150.45%,the buds were strong and grew well.The optimal medium for budding proliferation was MS+6-BA (0.5 mg?L-1)+NAA (0.2 mg?L-1),the multiplication rate was 4.03 ± 0.43,the plant was strong,the leaves were dark green and grew well.The best medium for rooting culture was MS+NAA ( 0.2 mg?L-1)+IBA ( 0.4 mg?L-1),the rooting rate was over 85% after 30 days.Then transplanted to sterilized perlite∶peat∶sand=1∶1∶1 for colonization,and the survival rate was 85%.
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备注/Memo
第一作者简介：官锦燕（1987-），女，硕士，农艺师，现主要从事珍稀热带植物选育等研究工作。E-mail：guanjinyan1987@163.com.责任作者：罗青文（1988-），男，博士，高级农艺师，现主要从事遗传育种等研究工作。E-mail：qwluoya@163.com.基金项目：湛江市科技计划资助项目(2020B01425,2022A01035）；湛江市“领航计划”资助项目（2020LHJH006）。收稿日期：2022-08-18