LUO Taimin,XU Wen.Effects of Darkness Treatment on Growth of Tomato Seedlings Based on Tissue Culture[J].Northern Horticulture,2023,(10):46-51.[doi:10.11937/bfyy.20223312]
基于组织培养的黑暗处理对番茄幼苗生长的影响
- Title:
- Effects of Darkness Treatment on Growth of Tomato Seedlings Based on Tissue Culture
- 文献标志码:
- A
- 摘要:
- 以番茄(Lycopersicon esculentum)幼苗为试材，采用组织培养方法，研究黑暗处理对番茄幼苗表型、生长指标和自噬作用相关基因SlATG8f表达的影响，以期为进一步研究黑暗胁迫下自噬作用相关基因的表达调控机制提供参考依据。结果表明：黑暗环境条件下番茄幼苗下胚轴显著伸长，叶柄伸长变缓，叶绿素合成减少，对幼苗的根鲜质量无显著影响，但是导致植物地上部生长受到抑制；叶绿素含量下降是黑暗诱导的叶片衰老最明显的表现，在3～5 d的黑暗处理过程中，自噬相关基因SlATG8f对相对早期的黑暗处理有明显响应。
- Abstract:
- Taking tomato (Lycopersicon esculentum) seedlings as test materials,tissue culture was used,the effects of darkness treatment on the phenotype,growth index and expression of autophagy-related gene SlATG8f in tissue culture were studied,in order to provide reference for further study on the expression and regulation mechanism of autophagy-related genes under darkness stress.The results showed that the hypocotyls of tomato seedlings elongated significantly,the petioles elongated slowly,and the synthesis of chlorophyll decreased under dark condition.There was no significant effect on the root fresh weight of tomato seedlings,but the growth of shoot was inhibited.The decline of chlorophyll content was the most obvious manifestation of dark-induced senescence,and the autophagy-related gene SlATG8f had a significant response to the relatively early dark treatment during the 3-5 days dark treatment.
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备注/Memo
第一作者简介：罗太敏（1996-），女，硕士研究生，研究方向为蔬菜生长发育生理及调控。E-mail：1838195635@qq.com.责任作者：须文（1975-），女，博士，教授，现主要从事蔬菜逆境响应生理及调控机制等研究工作。E-mail：wxu1@gzu.edu.cn.基金项目：国家自然科学基金资助项目（31760594）；贵州省“千”层次人才培养资助项目（贵大“千”层次[2018]02号）。收稿日期：2022-08-12