SHI Chenyu,HUANG Gangping.Effects of Oxytetracycline Stress on Physiological Characteristics of Cucumber Seedlings[J].Northern Horticulture,2023,(06):1-6.[doi:10.11937/bfyy.20222490]
土霉素胁迫对黄瓜幼苗生理特性的影响
- Title:
- Effects of Oxytetracycline Stress on Physiological Characteristics of Cucumber Seedlings
- 文献标志码:
- A
- 摘要:
- 以水果黄瓜为试材，采用土壤盆栽试验，研究了不同浓度土霉素（5、10、20、40、80 mg?L-1）胁迫对黄瓜种子萌发、幼苗形态、渗透调节物质含量以及抗氧化酶活性等生理特性的影响，以期为土霉素的合理使用提供参考依据。结果表明：土霉素胁迫对黄瓜种子萌发和幼苗生长的影响整体表现为抑制作用。土霉素对黄瓜种子发芽率的影响不显著，较高浓度（≥40 mg?L-1）土霉素降低了发芽势、胚根长度以及幼苗的形态生长、叶绿素含量和过氧化物酶（POD）活性。不同浓度土霉素胁迫提高了黄瓜幼苗可溶性糖、可溶性蛋白质（SP）、脯氨酸（Pro）、抗坏血酸（AsA）、丙二醛（MDA）含量以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT)活性。
- Abstract:
- Taking fruit cucumber as experimental material，the effects of different concentrations of oxytetracycline (5，10，20，40，80 mg?L-1) stress on the physiological characteristics of cucumber，such as seed germination，seedling morphology，osmoregulation substance content and antioxidant enzyme activity were studied by using soil pot experiment,in order to provide reference for the rational use of oxytetracycline.The results showed that the effect of oxytetracycline stress on cucumber seed germination and seedling growth was inhibition.The effect of oxytetracycline on the germination rate of cucumber seeds was not significant.The higher concentration (≥40 mg?L-1) of oxytetracycline reduced the germination potential，radicle length，seedling morphological growth，chlorophyll content and POD activity.Different concentrations of oxytetracycline stress increased the contents of soluble sugar，soluble protein (SP)，proline (Pro)，ascorbic acid (AsA)，malondialdehyde (MDA) and the activities of SOD and CAT in cucumber seedlings.
参考文献/References:
[1]张春艳.土霉素与锌胁迫对青菜生长及抗氧化系统酶的影响[D].合肥:安徽农业大学,2012.[2]支苏丽,张克强.兽用抗生素污染特点与控制技术[J].中国农业科学,2015(S1):104-112.[3]李红娜,阿旺次仁,李斌绪,等.兽用抗生素研究的文献计量学分析[J].农业环境科学学报,2017,267(11):2297-2306.[4]崔馨,乔显亮,韩成伟,等.生菜对土霉素的吸收及其植物毒性[J].农业环境科学学报,2008(3):1038-1042.[5]张俊亚,魏源送,陈梅雪,等.畜禽粪便生物处理与土地利用全过程中抗生素和重金属抗性基因的赋存与转归特征研究进展[J].环境科学学报,2015,35(4):935-946.[6]ZHANG Q Q,YING G G,PAN C G,et al.Comprehensive evaluation of antibiotics emission and fate in the river basins of china:Source analysis，multimedia modeling，and linkage to bacterial resistance[J].Environmental Science & Technology,2015,49(11):6772-6782.[7]赵方凯,杨磊,李守娟,等.长三角典型城郊土壤抗生素空间分布的影响因素研究[J].环境科学学报,2018,38(3):1163-1171.[8]JOANNE C,CHEE S,RODERICK I,et al.Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste[J].Journal of Environmental Quality,2009,38(3):1086-1108.[9]饶怡璇,汪斌,何玉洁,等.抗生素在土壤中的归趋及不可提取态残留[J].环境化学,2022,41(2):549-560.[10]HAMSCHER G,PAWELZICK H T,HOPER H,et al.Different behavior of tetracyclines and sulfonamides in sandy soils after repeated fertilization with liquid manure[J].Environ Toxicol Chem,2005,24(4):861-868.[11]罗凯,李文红,章海波,等.南京典型设施菜地有机肥和土壤中四环素类抗生素的污染特征调查[J].土壤,2014,46(2):330-338.[12]刘彩媚,林泳欣,谢晓仪,等.广州市城-郊梯度上典型蔬菜地土壤抗生素污染研究[J].广东农业科学,2019,46(6):59-67.[13]莫苑敏.广西青狮潭水库喹诺酮类抗生素残留特征及风险评价[D].桂林:桂林理工大学,2019.[14]程兆康,杨金山,吕敏,等.我国畜禽养殖业抗生素的使用特征及其环境与健康风险[J].农业资源与环境学报,2022,39(6):1253-1262.[15]王冲,罗义,毛大庆.土壤环境中抗生素的来源、转归、生态风险以及消减对策[J].环境化学,2014,33(1):19-29.[16]MINDEN V,DELOY A,VOLKERT A M,et al.Antibiotics impact plant traits，even at small concentrations[J].AoB Plants,2017(2):2.[17]BOXALL A B,JOHNSON P,SMITH E J,et al.Uptake of veterinary medicines from soils into plants[J].Agric Food Chem,2006,54(6):2288-2297.[18]王磊,王金花,王军,等.四种抗生素对小麦玉米高粱三种作物种子芽与根伸长的影响[J].农业环境科学学报,2017,36(2):216-222.[19]马莎.四环素和重金属（Cu2+、Cd2+）单一及复合污染对小白菜的毒性效应研究[D].雅安:四川农业大学,2013.[20]范惠冬,王厚继,李阿红,等.粪肥中残留抗生素对不同蔬菜种子萌发过程中胚根伸长及子叶叶绿素合成的影响[J].黑龙江农业科学,2022(3):54-59.[21]廖德润,刘超翔,王振,等.兽用抗生素胁迫对空心菜的影响研究[J].环境科学学报,2013,33(9):2558-2564.[22]鲍陈燕,顾国平,章明奎.兽用抗生素胁迫对水芹生长及其抗生素积累的影响[J].土壤通报,2016,47(1):164-172.[23]宋建丽,秦俊梅,熊华烨,等.添加不同基质对含四环素土壤玉米幼苗生理特性的影响[J].水土保持学报,2019,33(2):342-349.[24]饶玲.不同外源硒对干旱胁迫下黄瓜生长的影响[D].重庆:西南大学,2017.[25]郑曦，张凯.常见抗生素对玉米种子萌发和幼苗生长的影响[J].种子,2019,38(11):51-56.[26]秦俊梅,卜玉山,李兆君,等.土霉素胁迫对大豆和小麦幼苗生长的影响[J].山西农业大学学报(自然科学版),2012,32(2):103-106.[27]赵姗姗.MicroRNA对铁观音茶树干旱胁迫响应机制研究[D].福州:福建农林大学,2015.[28]王学奎.植物生理生化实验原理和技术[M].2版.北京:高等教育出版社,2006.[29]董亚茹,张艳波,赵东晓,等.外源24-表油菜素内酯对NaCl胁迫下桑树幼苗的缓解效应[J].核农学报,2021,35(6):1466-1475.[30]刘新,刘洪庆.生物学实践教学改革系列教材植物生理学实验[M].北京:高等教育出版社,2018.[31]张天莹.两种典型抗生素对小麦幼苗生态毒性效应的影响[D].扬州:扬州大学,2020.[32]徐恒恒,黎妮,刘树君,等.种子萌发及其调控的研究进展[J].作物学报,2014,40(7):1141-1156.[33]冯晶晶,温洪宇,孟艳秋,等.3种抗生素胁迫对黄瓜、西葫芦和冬瓜种子萌发的影响[J].种子,2018,37(2):86-89.[34]王朋,温蓓,张淑贞.环丙沙星对玉米芽期抗氧化酶活性及自由基代谢的影响[J].环境化学,2011,30(4):753-759.[35]张继旭,张继光,孔凡玉,等.四环素对土壤碳氮矿化及烟草种子萌发的影响[J].江苏农业科学,2018,46(15):54-58.[36]魏瑞成,邵明诚,陈明,等.金霉素和4-差向金霉素对油菜生长的影响及其在幼苗体内的积累[J].农业环境科学学报,2012,31(7):1289-1295.[37]郑阳霞.枸杞类胡萝卜素合成酶基因（PSY、LycB）的克隆及其转化洋桔梗的研究[D].雅安:四川农业大学,2006.[38]周品成,刘希强,康兴生,等.4种水生植物对兽用抗生素去除效果比较[J].华南农业大学学报,2019,40(6):67-73.[39]张友旺.含四环素的有机肥对土壤微生物及小白菜生长的影响[D].杨凌:西北农林科技大学,2013.[40]赵月.两种典型抗生素在土壤-植物体系中的迁移及生态毒性研究[D].石家庄:河北科技大学,2016.[41]ROSA M,PRADO C,PODAZZA G,et al.Soluble sugars--metabolism，sensing and abiotic stress:A complex network in the life of plants[J].Plant Signal Behav,2009,4(5):388-393.[42]潘东琪.土霉素与差向土霉素对小白菜幼苗生长的生态毒性研究[D].哈尔滨:东北农业大学,2017.[43]贲蓓倍,刘海学,赵里曼,等.不同玉米品种对磺胺类抗生素复合污染的生理响应及抗性评价[J/OL].分子植物育种,(2021-08-05)[2022-04-01].DOI:https://kns.cnki.net/kcms/detail/46.1068.S.20210804.1110.002.html.〖JP〗[44]KAVI KISHOR P B,HIMA KUMARI P,SUNITA M S,et al.Role of proline in cell wall synthesis and plant development and its implications in plant ontogeny[J].Front Plant Sci,2015(6):544.[45]杨舒贻,陈晓阳,惠文凯,等.逆境胁迫下植物抗氧化酶系统响应研究进展[J].福建农林大学学报(自然科学版),2016,45(5):481-489.[46]陈智鑫,李学鹏,彭鸿旭,等.4种抗生素对桑树种子萌发的影响[J].种子,2018,37(1):8-13.[47]刘婧,孙培琪,周广芳,等.枣树叶片衰老过程中丙二醛含量和抗氧化酶活性的变化[J].落叶果树,2011,43(2):1-3.[48]严萍.含腐殖酸调理剂对小麦、水稻幼苗生长和产量的影响[D].扬州:扬州大学,2016.[49]迟荪琳,王卫中,徐卫红,等.四环素类抗生素对不同蔬菜生长的影响及其富集转运特征[J].环境科学,2018,39(2):935-943.[50]陈博阳,余彬彬,钱晓晴,等.锌和土霉素胁迫对玉米种子发芽和幼苗抗氧化酶活性的影响[J].江苏农业学报,2017,33(1):13-18.[51]凌德.土霉素和磺胺二甲嘧啶胁迫下辣椒生理响应及土壤理化性质研究[D].雅安:四川农业大学,2015.[52]武剑,DEBELA S A,华倩雯,等.外源抗生素对栽培作物与野生植物的氧化胁迫及其富集转运的差异性[J].南京师大学报(自然科学版),2020,43(2):84-91.[53]金明康.喹诺酮类抗生素对拟南芥及线蚓的生态毒理机制及抗性组学研究[D].杭州:浙江工商大学,2021.
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备注/Memo
第一作者简介：史沉鱼（1982-），女，博士，副教授，现主要从事植物生理学等研究工作。E-mail：79490466@qq.com.基金项目：广西教育厅科研资助项目（2021KY0623）；河池学院科研资助项目（2021GCC023）；“桂西北特色植物资源开发与功能研究中心”研究平台资助项目（2017-05-02）。收稿日期：2022-06-16