(College of Resources and Environment,Northeast Agricultural University,Harbin,et al.YAN Haohao,MI Yaozu,SANG Mingyu,ZANG Hailian,LI Chunyan[J].Northern Horticulture,2023,(05):39-46.[doi:10.11937/bfyy.20222430]
蓝靛果叶斑病病原高粱附球菌(Epicoccum sorghinum)分离鉴定及生物学特性
- Title:
- YAN Haohao,MI Yaozu,SANG Mingyu,ZANG Hailian,LI Chunyan
- Keywords:
- blue honeysuckle (Lonicera caerulea L.); leaf spot disease; Epicoccum sorghinum; isolation and identification; biological characterization
- 文献标志码:
- A
- 摘要:
- 以黑龙江省哈尔滨市香坊区采集的蓝靛果发病植株为试材,通过病原菌分离培养、致病性测定、形态学鉴定和rDNA-ITS、LSU、TUB和RPB2序列分析,确定其分类地位,以期为将来实施有效的化学和生物防治提供重要病原菌株来源。结果表明:该病原菌为高粱附球菌(Epicoccum sorghinum),可在PDA、OA和MEA培养基上生长,最适培养基为OA。最适培养条件为生长温度25 ℃,pH 7,碳源为乳糖和葡萄糖,氮源为牛肉膏,并且全光照条件有利于菌丝生长。
- Abstract:
- Infected blue honeysuckle (Lonicera caerulea L.) plants collected in Xiangfang district,Harbin,Heilongjiang Province,were used as test materials.The classification status of pathogen strain was determined by isolation and culture of pathogenic fungus,pathogenicity determination,morphological identification and rDNA-ITS,LSU,TUB and RPB2 sequence analysis,in order to provide an important source of pathogen strain for future effective chemical and biological control.The results showed
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备注/Memo
第一作者简介:闫浩浩(1996-),男,博士研究生,研究方向为植物病害病原物分离鉴定与防治。E-mail:17863801132@163.com.责任作者:李春艳(1970-),女,博士,教授,博士生导师,现主要从事植物病害病原物分离鉴定与防治等研究工作。E-mail:chunyanli@neau.edu.cn.基金项目:国家自然科学基金资助项目(42071268)。收稿日期:2022-06-13