[1]刘月芹,罗丽媛,刘欣瑞,等.林下种植灵芝内生细菌和放线菌的分离鉴定[J].北方园艺,2023,(03):120-126.[doi:10.11937/bfyy.20222032]
　LIU Yueqin,LUO Liyuan,LIU Xinrui,et al.Isolation and Identification of Endophytes and Actinomycetes From Underwood Planting Ganoderma lucidum[J].Northern Horticulture,2023,(03):120-126.[doi:10.11937/bfyy.20222032]
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林下种植灵芝内生细菌和放线菌的分离鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年03 页码: 120-126 栏目: 出版日期: 2023-02-15
Title:
Isolation and Identification of Endophytes and Actinomycetes From Underwood Planting Ganoderma lucidum
作者:
刘月芹; 罗丽媛; 刘欣瑞; 白鹭
（延安大学 生命科学学院，陕西 延安 716000）
Author(s):
LIU Yueqin; LUO Liyuan; LIU Xinrui; BAI Lu
(College of Life Science,Yan′an University,Yan′an,Shaanxi 716000)
关键词:
灵芝; 内生菌; 分离; 鉴定
Keywords:
Ganoderma lucidum; endophytes; isolation; identification
DOI:
10.11937/bfyy.20222032
文献标志码:
A
摘要:
以林下种植灵芝为试材，采用16S rDNA鉴定方法，研究了不同分离方法对灵芝内生细菌和放线菌多样性的影响，以期能够发现产生活性成分的菌种或者新种，从而推动微生物医药的发展进程。结果表明：从健康的灵芝子实体内分离得到放线菌1门1属的4个菌株(LZ-F1、LZ-F2、LZ-F3、LZ-F4)和细菌2门4属的5个菌株(LZ-B1、LZ-B2、LZ-B3、LZ-B4、LZ-B5)。4株放线菌均属于链霉菌属(Streptomyces)，5株细菌分别属于周杆菌属(Peribacillus)、假单胞菌属(Pseudomonas)、新根瘤菌属(Neorhizobium)和芽孢杆菌属(Bacillus)。该研究为其它食用菌内生菌的分离提供了技术支撑，也为灵芝活性成分的进一步开发利用提供了较好的参考价值。
Abstract:
The underwood planting Ganderma lucidum was used as test material,the effect of different separation methods on the diversity of endophytic bacteria and actinomycetes of G.lucidum was studied by using 16S rDNA identification method,in order to find the strains or new species that produce active ingredients to promote the development of microbial medicine.The results showed that four strains belonging to one genus and one phylum of actinomycetes (LZ-F1,LZ-F2,LZ-F3,LZ-F4) and five strains belonging to four genera and two phyla of bacteria (LZ-B1,LZ-B2,LZ-B3,LZ-B4,LZ-B5) were isolated from healthy G.lucidum fruiting bodies.All four actinomycetes belong to Streptomyces,five strains belong to Peribacillius,Pseudomonas,Neorrhizobium and Bacillus respectively.This study provided technical support for the isolation of other edible fungi,and provided a good reference value for the further development and utilization of active ingredients of G.lucidum.

参考文献/References:

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备注/Memo

第一作者简介：刘月芹（1980-），女，博士，讲师，硕士生导师，现主要从事微生物资源的开发及食用菌栽培等研究工作。E-mail:liuyue811223@126.com.基金项目：国家自然科学基金资助项目（22268045）；延安大学博士科学研究启动资助项目（YDBK2015-08）；陕西省科学技术厅资助项目（2020NY-078）。收稿日期：2022-05-17

更新日期/Last Update: 2023-03-07