JIN Li,LI Bin,TIAN Fang,et al.Comparative Experiment on Efficient Tissue Isolation of Dried Phellinus igniarius[J].Northern Horticulture,2023,(01):113-118.[doi:10.11937/bfyy.20221641]
高效组织分离桑黄干菇的比较试验
- Title:
- Comparative Experiment on Efficient Tissue Isolation of Dried Phellinus igniarius
- 文献标志码:
- A
- 摘要:
- 以桑黄为试材，通过对比试验，对桑黄干菇组织分离的过程进行优化，以期提高分离成功率。结果表明：提前用水对组织块浸泡2~3 h，用75%酒精浸泡杀菌15~30 s，PDA培养基中加入抗生素，可以大幅度提高分离成功率，达到80%以上，甚至高达100%。
- Abstract:
- The tissue separation process of dried mushroom of Phellinus igniarius was optimized through comparative experiments with Phellinus igniarius as the experimental material,in order to improve the separation success rate.The results showed that through the comparison of this experiment,the process of tissue isolation of dried mushroom Phellinus igniarius was optimized.It could be seen that the tissue block was soaked in water for 2-3 hours in advance,the tissue block was soaked in 75% alcohol for sterilization for 15-30 seconds,and antibiotics were added in PDA medium,which could greatly improve the success rate of separation,which could reach 80% or even up to 100%.
参考文献/References:
[1]刘惠知,邵晨霞,吴胜莲,等.桑黄菌的药理及产品开发研究进展[J].食用菌,2013(5):1-3.[2]袁华军.抗生素在制食用菌母种中的应用[J].四川农业科技,2014(7):35.[3]侯军,张春梦,林晓民,等.抗生素培养基对高羊肚菌菌丝生长的影响[J].食用菌,2010(1):35-36.[4]黎欢吉.环境因子对野生桑黄菌丝生长的影响[J].山西农业科学,2015,43(9)：1146-1148,1159.
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备注/Memo
第一作者简介：金丽（1990-），女，硕士，研究实习员，现主要从事食用菌新品种选育及栽培技术等研究工作。E-mail：1213750713@qq.com.责任作者：李宾(1984-)，男，本科，助理研究员，现主要从事食用菌新品种选育及栽培技术等研究工作。E-mail：libill0371@126.com.收稿日期：2022-04-22