[1]曾洪学,屈兴红.葛藤对低温胁迫的生长和生理生化响应[J].北方园艺,2022,(18):109-116.[doi:10.11937/bfyy.20220955]
　ZENG Hongxue,QU Xinghong.Growth，Physiology and Biochemistry Responses of Pueraria lobata to Low Temperature Stress[J].Northern Horticulture,2022,(18):109-116.[doi:10.11937/bfyy.20220955]

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葛藤对低温胁迫的生长和生理生化响应

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年18 页码: 109-116 栏目: 出版日期: 2022-09-30

Title:: Growth，Physiology and Biochemistry Responses of Pueraria lobata to Low Temperature Stress

作者:: 曾洪学; 屈兴红; （浙江同济科技职业学院，浙江杭州 311231）

Author(s):: ZENG Hongxue; QU Xinghong; (Zhejiang Tongji Vocational College of Science and Technology，Hangzhou，Zhejiang 311231)

关键词:: 葛藤; 低温胁迫; 生长; 生理生化

Keywords:: Pueraria lobata; low temperature stress; growth; physiological and biochemical

DOI:: 10.11937/bfyy.20220955

文献标志码:: A

摘要:: 以湖南和澳大利亚葛藤种质为试材，采用低温梯度试验处理的方法，研究了人工模拟温度25、15 ℃和10 ℃ 3个温度梯度下2份葛藤种质的生长、生理生化变化及耐低温能力，以期为葛藤利用提供参考依据。结果表明：2份葛藤种质的生长指标（生长速度、鲜质量、干质量、叶面积、叶宽、叶长、叶周长）均随温度的降低而下降；电导率、丙二醛含量、渗透调节物质含量（脯氨酸、可溶性糖）、抗氧化酶活性（过氧化氢酶、过氧化物酶、超氧化物歧化酶、抗坏血酸过氧化物酶）均随温度的降低而升高；脱落酸含量升高，而赤霉素、生长素和玉米素含量随温度的降低而降低；低温条件下澳大利亚种质的各项生长指标、渗透调节物质含量、抗氧化酶活性和内源激素含量基本较湖南种质高。综上表明，2份葛藤种质通过减缓植物的生长速度、降低生物量、改变叶形态参数、降低植物内源激素含量、提高渗透调节物质和增加抗氧化酶活性来适应低温胁迫。而低温胁迫下累积或维持较高的渗透调节物质含量、抗氧化酶活性和内源激素含量可能是澳大利亚种质耐寒性较湖南种质强的主要原因。

Abstract:: Taking Hunan and Australian Pueraria lobata germplasms as experimental materials，the growth，physiological and biochemical changes and low temperature tolerance of two Pueraria lobata germplasms under artificial simulated temperature gradients of 25 ℃，15 ℃ and 10 ℃ were studied and analyzed by using the method of low temperature gradient test，in order to provide reference for the utilization of Pueraria lobata.The results showed that the growth indexes (growth rate，fresh weight，dry weight，leaf area，leaf width，leaf length and leaf perimeter) of the two Pueraria lobata germplasms decreased with the decrease of temperature.Conductivity，malondialdehyde content，osmotic adjustment substance content (soluble sugar，proline)，antioxidant enzyme activity (catalyst，peroxidase，superoxide dismutase，ascorbate peroxidase) all increased with the decrease of temperature.The contents of ABA increased，while the contents of GA3，IAA and ZT decreased with the decrease of temperature.Under low temperature conditions，the growth indicators，osmotic adjustment material content，antioxidant enzyme activity and endogenous hormone content of Australian germplasm were basically higher than those of Hunan germplasm.In conclusion，the two Pueraria lobata germplasms adapt to low temperature stress by slowing down the growth rate of plants，reducing biomass，changing leaf morphological parameters，reducing the content of endogenous hormones，improving osmotic adjustment substances and increasing the activity of antioxidant enzymes.Under low temperature stress，the accumulation or maintenance of higher osmotic adjustment material content，antioxidant enzyme activity and endogenous hormone content may be the main reason why Australian germplasm has stronger cold tolerance than Hunan germplasm.

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备注/Memo

第一作者简介：曾洪学(1974-)，男，硕士，副教授，现主要从事植物生理和植物生态及设施农业的教学和研究等工作。E-mail：zenghongxue@zjtongji.edu.cn.责任作者：屈兴红(1979-)，女，硕士，副教授，现主要从事植物逆境生理及农业生态环境的教学和研究等工作。E-mail：quxinghong@zjtongji.edu.cn.基金项目：浙江省高校基本科研经费资助项目（FRF20PY005）。收稿日期：2022-03-15

更新日期/Last Update: 2022-11-30