YANG Shenming,NING Na,YANG Xiao,et al.Optimization of Polyphenols Extraction From Schefflera arboricola Hayata by Response Surface Methodology and Its Antioxidant Activity[J].Northern Horticulture,2022,(18):90-98.[doi:10.11937/bfyy.20220725]
响应面法优化七叶莲多酚提取工艺及抗氧化性研究
- Title:
- Optimization of Polyphenols Extraction From Schefflera arboricola Hayata by Response Surface Methodology and Its Antioxidant Activity
- Keywords:
- Schefflera arboricola Hayata; polyphenols; ultrasound extraction; response surface methodology; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 以七叶莲为试材，采用纤维素酶协同超声辅助提取七叶莲多酚，通过单因素试验和响应面试验优化提取条件，研究了七叶莲多酚对羟基自由基（·OH）、DPPH 自由基（DPPH·）和亚硝酸盐（NO-2）的清除能力，以期为综合开发利用七叶莲多酚提供参考依据。结果表明：七叶莲多酚最佳提取工艺条件为乙醇体积分数 40％，料液比1∶35 g·mL-1，酶解时间35 min，超声功率350 W，酶解温度50 ℃，纤维素酶用量3.0％（以七叶莲干粉质量为基准，下同）；在该条件下，七叶莲多酚提取量为（15.52±0.824）mg·g-1，与预测值（15.61 mg·g-1）接近；七叶莲多酚对·OH、DPPH·和NO-2有较〖JP3〗强的清除能力，且随多酚质量浓度的增大而逐渐增强；七叶莲多酚质量浓度为0.137 mg·mL-1〖JP〗时，对·OH、DPPH·和NO-2的清除率分别为 54.33%、91.14%和61.42%。采用响应面法优化的多酚提取工艺参数准确可靠，可用于七叶莲多酚提取；七叶莲多酚具有较强的体外抗氧化能力，可作为食品及医药的天然抗氧化剂资源进行开发利用。
- Abstract:
- Taking Schefflera arboricola Hayata as the test material，extraction of polyphenols from Schefflera arboricola Hayata by cellulase combined with ultrasound was studied，the extraction conditions were optimized by univariate and response tests and the scavenging capacity of polyphenols from Schefflera arboricola Hayata on hydroxyl radicals (·OH)，DPPH radicals (DPPH·) and nitrite (NO-2) was also studied,in order to provide reference for the comprehensive development and utilization of Schefflera arboricola Hayata polyphenols.The results showed that the optimum extraction conditions of polyphenols from Schefflera arboricola Hayata were ethanol volume fraction 40%，solid-liquid ratio 1:35 g·mL-1，enzymolysis hydrolysis time 35 minutes，ultrasonic power 350 W，enzymolysis temperature 50 ℃，cellulase dosage 3.0%(with the quality of Schefflera arboricola Hayata，the same below).Under these conditions，the extraction amount of polyphenols from Schefflera arboricola Hayata was (15.52±0.824) mg·g-1，which was close to the predicted value (15.61 mg·g-1).Schefflera arboricola Hayata polyphenols showed strong scavenging effects on hydroxyl radicals，DPPH radicals and nitrite，and increased with the increase of polyphenols concentration.When the concentration of Schefflera arboricola Hayata polyphenols was 0.137 mg·mL-1，the clearance rates of ·OH，DPPH· and NO-2 were 54.33%，91.14% and 61.42% respectively.The extraction process parameters optimized by response method were accurate and reliable，and could be used for the extraction of polyphenols from Schefflera arboricola Hayata.Schefflera arboricola Hayata polyphenols had strong antioxidant capacity in vitro and could be used as natural antioxidant resources in food and medicine.
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备注/Memo
第一作者简介：杨申明（1976-），男，本科，高级实验师，现主要从事天然产物化学等研究工作。E-mail:ysm@cxtc.edu.cn.责任作者：王振吉（1983-），男，博士，教授，现主要从事天然产物化学成分和药理活性等研究工作。E-mail:wangzj@cxtc.edu.cn.基金项目：云南省中青年学术和技术带头人后备人才资助项目（2019HB055）；云南省“高层次人才培养支持计划”青年拔尖人才资助项目（YNWR-QNBJ-2020-104）；“彝乡科技领军人才”资助项目；楚雄师范学院开放实验室资助项目。收稿日期：2022-03-01