[1]张丰寅,杨再强,杨立,等.高温高湿对苗期黄瓜叶片光合特性和[J].北方园艺,2022,(16):1-8.[doi:10.11937/bfyy.20215161]
　ZHANG Fengyin,YANG Zaiqiang,YANG Li,et al.Effects of High Temperature and Humidity on Photosynthetic Characteristics and Protective Enzyme Activities of Cucumber Leaves at Seedling Stage[J].Northern Horticulture,2022,(16):1-8.[doi:10.11937/bfyy.20215161]

点击复制

高温高湿对苗期黄瓜叶片光合特性和

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年16 页码: 1-8 栏目: 出版日期: 2022-08-30

Title:: Effects of High Temperature and Humidity on Photosynthetic Characteristics and Protective Enzyme Activities of Cucumber Leaves at Seedling Stage

作者:: 张丰寅1; 杨再强1; 2; 杨立1; 罗靖1; 李岩宸1; 李春影1; （1．南京信息工程大学气象灾害预报预警与评估协同创新中心，江苏南京 210044；2.江苏省农业气象重点实验室，江苏南京 210044）

Author(s):: ZHANG Fengyin1; YANG Zaiqiang1; 2; YANG Li1; LUO Jing1; LI Yanchen1; LI Chunying1; (1.Collaborative Innovation Center for Meteorological Disaster Prediction,Early Warning and Evaluation,Nanjing University of Information Engineering,Nanjing,Jiangsu 210044；2.Jiangsu Key Laboratory of Meteorological,Nanjing,Jiangsu 210044)

关键词:: 高温高湿; 黄瓜; 光合特性; 保护酶

Keywords:: high temperature and high humidity; cucumber; photosynthetic characteristics; protective enzymes

DOI:: 10.11937/bfyy.20215161

文献标志码:: A

摘要:: 以“津优101”黄瓜为试材，于2020年5—9月在南京信息工程大学农业气象实验室内采用人工环境控制试验，研究高温高湿对苗期黄瓜光响应曲线、过氧化氢酶（CAT）活性、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、丙二醛（MDA）含量的影响，以期为高温高湿气象灾害监测预警及环境优化调控提供参考依据。结果表明：相同湿度下，叶片净光合速率（Pn）随着高温胁迫程度的增加而降低。3种酶活性随着高温胁迫程度的增加而增加。MDA含量随着高温胁迫程度的增加也逐渐升高。相同温度下，Pn随着湿度胁迫程度的增加而降低，但变化程度相比于温度胁迫较小。3种酶活性随着湿度胁迫程度的增加先升高后降低。MDA含量在70%湿度处理下最高。高温高湿复合处理下，Pn随着胁迫温度的升高、湿度的增加而降低。3种酶活性的变化与高温胁迫下的变化一致。MDA含量随着高温对黄瓜幼苗的损害程度增加，其抗逆性也随之增加。高温胁迫对植物净光合速率的影响相比于湿度胁迫更大，在70%湿度处理下净光合速率受高温胁迫影响降低，70%的湿度处理能够缓解高温危害。

Abstract:: Taking ‘Jinyou 101’ cucumber as test material,the effects of high temperature and high humidity on the light response curve,catalase activity (CAT),superoxide dismutase activity (SOD),peroxidase activity (POD) and malonaldehyde (MDA) content of cucumber seedlings were studied in the Meteorological Laboratory of Nanjing University of Information Technology from May to September 2020,in order to provide reference for monitoring and early warning of high temperature and high humidity meteorological disasters and environmental optimization and control.The results showed that under the same humidity,the net photosynthetic rate (Pn) of leaves decreased with the increasing of high temperature stress.The activities of the three enzymes increased with the increasing of the degree of high temperature stress.MDA content increased with the increase of high temperature stress.At the same temperature,Pn decreased with the increase of humidity stress,but the degree of change was smaller than that of temperature stress.The activities of the three enzymes first increased and then decreased with the increasing of humidity stress.MDA content was the highest under 70% humidity treatment.Under the combined treatment of high temperature and high humidity,Pn decreased with the increase of stress temperature and humidity.The changes of the three enzymes activities were consistent with those under high temperature stress.The content of MDA increased with the damage of high temperature to cucumber seedlings,and its stress resistance also increased.Conclusion：the effect of high temperature stress on the net photosynthetic rate of plants was greater than that of humidity stress.The net photosynthetic rate decreased under 70% humidity treatment,and 70% humidity treatment could alleviate the harm of high temperature.

参考文献/References:

[1]马俊礼.甘肃省设施黄瓜栽培现状与建议[J].农家参谋,2021(7):57-58.[2]崔庆梅,吴利荣,陈斐，等.高温胁迫对黄瓜幼苗生理生化及光合作用的影响[J].延安大学学报(自然科学版),2021,40(1):23-26,31.[3]潘璐.高温、CO2耦合条件下温室黄瓜光合作用调节机制及蛋白质组学研究[D].呼和浩特:内蒙古农业大学,2018.[4]李娥,赵锦,叶清，等.全球气候变暖对中国种植制度可能影响ⅩⅢ.东北三省春玉米熟型调整的降水限制及其对产量的可能影响[J].中国农业科学,2021,54(18):3847-3859.[5]ASHRAF M,HARRIS P J C.Photosynthesis under stressful environments：An overview[J].Photosynthetica：International Journal for Photosynthesis Research,2013,51(2):10-32.[6]田婧.外源亚精胺缓解黄瓜幼苗高温胁迫伤害的生理调节机制和蛋白质组学研究[D].南京:南京农业大学,2012.[7]孙胜楠.设施黄瓜对高温胁迫的响应与适应[D].泰安:山东农业大学,2017.[8]崔庆梅,吴利荣,陈斐,等.高温胁迫对黄瓜幼苗生理生化及光合作用的影响[J].延安大学学报(自然科学版),2021,40(1):23-26,31.[9]仝培江,程智慧,孟焕文.黄瓜幼苗对高温高湿胁迫的生理响应[J].西北农林科技大学学报(自然科学版),2021,49(6):85-93,103.[10]ZHAO X,NISHIMURA Y,FUKUMOTO Y,et al.Effect of high temperature on active oxygen species,senescence and photosynthetic properties in cucumber leaves[J].Environmental and Experimental Botany,2010,70(2):53-69.[11]王立涵,王翔,李世斌，等.高温胁迫下外源物质对黄瓜幼苗叶绿素荧光和抗氧化酶活性的影响[J].安徽农学通报,2019,25(10):20-22,95.[12]郝婷,丁小涛,金海军，等.高温对黄瓜生长的影响及设施栽培对策[J].上海农业学报,2014,30(2):125-131.[13]韦婷婷,杨再强,王琳，等.玻璃温室和塑料大棚内逐时气温模拟模型[J].中国农业气象,2018,39(10):644-655.[14]李合生.植物生理生化试验原理和技术[M].北京：高等教育出版社,2000.[15]白永宏,赵赞延,王亚洁，等.狗头枣果实生长过程中保护酶活性变化规律[J].山西农业科学,2020,48(3):345-348.[16]赵炀,李永生,高秀峰.基于愈创木酚荧光减量准确测定过氧化物酶活性的新方法[J].分析化学,2015,43(7):1040-1046.[17]王群,刘朝巍,徐文娟.紫外分光光度法测定玉米过氧化氢酶活性新进展[J].中国农学通报,2016,32(15):159-165.[18]RANI R,KUMAR V,GUPTA P,et al.Potential use of Solanum lycopersicum and plant growth promoting rhizobacterial (PGPR) strains for the phytoremediation of endosulfan stressed soil[J].Chemosphere,2021,279:35-45.[19]孙涛.高温胁迫对植物生理影响研究进展[C].西安：第十届全国植物结构与生殖生物学学术研讨会,2012.[20]中国科学院上海植物生理研究所.现代植物生理学实验指南[M].北京：科学出版社,1999.[21]SUSZEK-OPATKABEATA,MALISZEWSKA-KORDYBACHBARBARA,KLIMKOWICZ-PAWLAS.The multifactorial assessment of the Zn impact on high and low temperature stress towards wheat seedling growth under diverse moisture conditions (optimal and wet) in three soils[J].Journal of Hazardous Materials,2021,416(1):35-41.[22]潘莉,李勇鹏,宁德鲁，等.不同保鲜方法对核桃青皮感官及核桃仁可溶性蛋白含量的影响[J].中国油脂,2021,46(9):103-107.[23]de CASTRO J N,MULLER C,ALMEIDA G M，et al．Physiological tolerance to drought under high temperature in soybean cultivars[J].Australian Journal of Crop Science,2019,13(6):23-30.[24]杨婷.膜脂过氧化对植物细胞的伤害[J].科技与创新,2018(8):61-62.[25]解玉玲.高温胁迫对植物生理影响的研究进展[J].吉林农业,2019(8):107-108.[26]CAMEJO D，JIM NEZ A，ALARC N J J，et al．Changes in photosynthetic parameters and antioxidant activities following heat-shock treatment in tomato plants[J].Functional Plant Biology,2006(3):177-187.[27]乃小英,马凌之,陈年来.基质湿度对黄瓜嫁接苗叶片膜脂过氧化及保护酶活性的影响[J].甘肃农业大学学报,2009,44(1):84-88.[28]MILOSTIN D,HAKAN A.The effect of salicylic acid on photosynthetic characteristics,growth attributes,and some antioxidant enzymes on parsley (Petroselinum crispum L.) under salinity stress[J].Gesunde Pflanzen,2021(2):42-45.[29]SUN Y,XU J,LIN X,et al.Effects of exogenous silicon on maize seed germination and seedling growth[J].Scientific Reports,2021,11(1):56-62.[30]马德华,孙其信.温度逆境对不同品种黄瓜幼苗膜保护系统的影响[J].西北植物学报,2001(4):656-661.[31]唐璇.不同种类蔬菜幼苗对多环芳烃胁迫的响应[D].南宁:广西大学,2018.[32]LI L,LIN H,BO Y,et al.Response of protective enzymes in western flower thrips (Thysanoptera：Thripidae) to two Leguminous plants[J].Environmental Entomology,2020,49(5):65-70.

相似文献/References:

[1]李亚芹,郑根昌.冬棚黄瓜的氮磷钾肥配施效应分析[J].北方园艺,2014,38(12):146.
　LI Ya-qin,ZHENG Gen-chang. Effect Analysis of Application of N,P and K Fertilizers on Cucumber in Winter Plastic Greenhouses [J].Northern Horticulture,2014,38(16):146.
[2]由玉卿,秦智伟,周秀艳,等.黄瓜果实中丙醇二酸含量的配合力分析[J].北方园艺,2014,38(10):19.
　YOU Yu-qing,QIN Zhi-wei,ZHOU Xiu-yan,et al.Analysis on Combining Ability of Tartronic Acid Content in Cucumber[J].Northern Horticulture,2014,38(16):19.
[3]张秋萍,祁红英,张珏,等.丛枝菌根制剂对黄瓜幼苗生长的影响[J].北方园艺,2014,38(14):37.
　ZHANG Qiu-ping,QI Hong-ying,ZHANG Yu,et al.Effect of Arbuscular Mycorrhizal Agent on Growth of Cucumber Seedling[J].Northern Horticulture,2014,38(16):37.
[4]韩志平,张海霞,刘渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
　HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(16):1.
[5]王昶童,曹刚强,梁丛敏,等.黄瓜NBS类抗病基因类似物的生物信息学分析[J].北方园艺,2014,38(14):101.
　WANG Chang-tong,CAO Gang-qiang,LIANG Cong-min,et al.Bioinformatics Analysis of the NBS Resistance Gene Analogs in Cucumber[J].Northern Horticulture,2014,38(16):101.
[6]张瑞芬,李红岭,李新旭,等.优比胶囊不同使用方法对黄瓜生长及产量的影响[J].北方园艺,2013,37(17):4.
　ZHANG Rui-fen,LI Hong-ling,LI Xin-xu,et al.Effect of Different Usage of UB Capsule on Growth and Yield of Cucumber[J].Northern Horticulture,2013,37(16):4.
[7]张雪艳,李堃,马丽娟,等.Ca2+调控对黄瓜幼苗耐盐性生理指标的影响[J].北方园艺,2013,37(13):5.
　ZHANG Xue-yan,LI Kun,MA Li-juan,et al.Effects of Calcium Regulation on the Physiological Properties of Cucumber Under Salt Stress[J].Northern Horticulture,2013,37(16):5.
[8]杨延杰,林多.邻苯二甲酸对蔬菜种子萌发和幼苗生长及根系形态的影响[J].北方园艺,2013,37(13):9.
　YANG Yan-jie,LIN Duo.Effects of Phthalic Acid on Seed Germination，Seedling Growth and Root Morphology of Cucumber and Radish[J].Northern Horticulture,2013,37(16):9.
[9]张建祥,贾利元.越冬茬黄瓜结瓜期管理技术[J].北方园艺,2013,37(13):63.
　ZHANG Jian-xiang,JIA Li-yuan.Management Techniques of Overwintering Cucumber During the Fruiting Period[J].Northern Horticulture,2013,37(16):63.
[10]裴倩如,朱本忠,田慧琴,等.冷激处理对黄瓜低温贮藏中冷害的影响[J].北方园艺,2014,38(01):127.
　PEI Qian-ru,ZHU Ben-zhong,TIAN Hui-qin,et al.Effect of Cold Shock Treatment on Chilling Injury of Cucumber Under Low Temperature Storage[J].Northern Horticulture,2014,38(16):127.

备注/Memo

第一作者简介：张丰寅（1998-），男，硕士研究生，研究方向为设施农业气象。E-mail：20211208020@nuist.edu.cn.责任作者：杨再强（1967-），男，博士，教授，现主要从事设施农业气象等研究工作。E-mail：yzq@nuist.edu.cn.基金项目：国家重点研究开发计划资助项目（2019YFD1002202）。收稿日期：2021-12-17

更新日期/Last Update: 2022-09-21