LIU Zhijie,ZHENG Yan,NIU Weikang,et al.Polyploid Induction and Identification of Two Wild Clematis[J].Northern Horticulture,2022,(18):52-59.[doi:10.11937/bfyy.20214981]
两种野生铁线莲多倍体诱导与鉴定
- Title:
- Polyploid Induction and Identification of Two Wild Clematis
- Keywords:
- Clematis urophylla Franch.; Clematis heracleifolia DC.; polyploid; colchicine; photosynthetic index
- 文献标志码:
- A
- 摘要:
- 秋水仙素处理对植株形态和成苗情况的影响显著，0.1%浓度的秋水仙素处理对植株生长具有促进作用，0.3%浓度的秋水仙素表现出较强的抑制作用。采用染色体计数鉴定、流式细胞仪鉴定等方式确定变异株的染色体加倍情况，结合植株生长状态发现秋水仙素浓度为0.2%、处理时间为48 h的诱变效果最佳。变异株的叶绿素含量增加、胞间二氧化碳浓度降低，气孔导度、净光合速率、蒸腾速率升高，证明变异株光合作用增强。
- Abstract:
- The germinating seeds of wild Clematis urophylla Franch. and Clematis heracleifolia DC.in Hebei Province were used as experimental materials,colchicine was used for polyploid induction,the mutagenesis effects of different treatment combinations on two kinds of Clematis were studied,in order to obtain polyploid materials of Clematis.The results showed that the effect of colchicine treatment on plant morphology and seedling formation was remarkable.It was found that 0.1% colchicine treatment had a certain promoting effect on plant growth,and 0.3% colchicine showed a strong inhibitory effect.The chromosome doubling of the mutant strain was determined by chromosome counting and flow cytometry,and combined with the growth status of the plant,it was found that the mutagenesis effect was the best when the concentration of colchicine was 0.2% and the treatment time was 48 hours.The chlorophyll content increased,intercellular carbon dioxide concentration decreased,stomatal conductance,net photosynthetic rate and transpiration rate increased,which proved that the photosynthesis of mutant was enhanced.The research results could provide reference and theoretical support for the polyploid breeding of Clematis.The polyploid materials of Clematis are expected to be popularized as a new variety after further cultivation.
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备注/Memo
第一作者简介：刘志杰（1997-），男，硕士研究生，研究方向为园林植物育种。E-mail：1412075561@qq.com.责任作者：刘冬云（1971-），女，博士，副教授，硕士生导师，现主要从事地被植物资源收集及育种等研究工作。E-mail：dongyunliu@hebau.edu.基金项目：河北省科技厅资助项目(1220256)。收稿日期：2021-12-07