FENG Zhengguang,WANG Xuezhong,LIU Xiaodong,et al.Isolation and Identification of Bacillus amyloliquefaciens 7T6 and Biological Control of Powdery Mildew of Honeysuckle[J].Northern Horticulture,2022,(11):95-103.[doi:10.11937/bfyy.20214251]
降香黄檀根瘤菌的分离、鉴定及生物学特性
- Title:
- Isolation and Identification of Bacillus amyloliquefaciens 7T6 and Biological Control of Powdery Mildew of Honeysuckle
- Keywords:
- Bacillus amyloliquefaciens 7T6; isolation; identification; powdery mildew of honeysuckle; control
- 文献标志码:
- A
- 摘要:
- 以3种不同石砾和壤土配比栽培下的降香黄檀幼苗根瘤为试材，分离纯化其内生根瘤菌，通过形态学观察、生物学特性分析和16S rDNA测序等方法，鉴定研究了降香黄檀在不同石砾含量栽培基质条件下内生根瘤菌的种类差异及生物学特性，以期为石漠化地区的降香黄檀推广种植提供候选菌株。结果表明：降香黄檀在全石砾栽培基质条件下的根瘤菌菌株为慢生根瘤菌属(Bradyrhizobium)和根瘤菌属(Rhizobium)，而在石砾和壤土混合基质以及全壤土基质的根瘤菌菌株均为根瘤菌属(Rhizobium)。该研究分离获得的根瘤菌具有较强的固氮、结瘤和耐高浓度钙能力；回接根瘤菌的降香黄檀幼苗主根长、植株鲜质量和干质量均显著增加(P<0.05)。不同石砾含量栽培基质条件下降香黄檀内生根瘤菌的种类具有差异且均能够固氮结瘤和促进植株生长，表明这些根瘤菌在石砾含量不同的石漠化土壤环境中具有潜在的应用前景。
- Abstract:
- Brown spot pathogen Phomopsis sp.,black spot pathogen Chaetomium bostrychodes,Fusarium oxysporum,Alternaria,and powdery mildew of Microsphaera lonicerae were used as materials.Plate confrontation culture and field effectiveness test methods were adopted to study the antagonistic activity and field control effect of rhizosphere soil microorganism of honeysuckle,in order to screen out a biocontrol potential bacterium with the best control effect on powdery mildew of honeysuckle in the field.The results showed that among the 6 strains of bacteria with biocontrol potential, Bacillus amyloliquefaciens 7T6 was the best one on control effect on powdery mildew of honeysuckle.The field randomized block experiments showed that on the 3rd day of the experiments,the control effect of Bacillus amyloliquefaciens 7T6 fermentation broth on powdery mildew of honeysuckle was slightly lower than that of 43% tebuconazole SC.When the experiments went on 7th day,the efficacy of Bacillus amyloliquefaciens 7T6 fermentation broth reached 81.22% and 88.47% at the dosage of 325 g?(15kg)-1 and 650 g?(15kg)-1,which was not significantly different from that of 43% tebuconazole SC control effect (87.40%).The results that although the fast-acting property of Bacillus amyloliquefaciens 7T6 fermentation broth was slightly lower than that of tebuconazole,but the efficacy persistence of Bacillus amyloliquefaciens 7T6 fermentation broth was obvious,and the effects were also significant on improving the color of honeysuckle leaves and promoting the growth of shoots.
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备注/Memo
第一作者简介：文黎(1996-)，女，硕士研究生，研究方向为森林培育。E-mail：3287367825@qq.com.责任作者：玉舒中(1980-)，男，硕士，高级实验师，现主要从事植物生理生态等研究工作。E-mail：1047918014@qq.com.基金项目：国家自然科学基金地区资助项目(31760058)。收稿日期：2021-10-20