YIN Xin,XU Liping,XUE Qianxin,et al.Relationship Between Rhizosphere Soil Factors and Medicinal Materials Quality of Bupleurum chinense DC.in Chengde Area[J].Northern Horticulture,2022,(08):95-101.[doi:10.11937/bfyy.20213917]
承德地区柴胡根际土壤因子与药材质量的关系
- Title:
- Relationship Between Rhizosphere Soil Factors and Medicinal Materials Quality of Bupleurum chinense DC.in Chengde Area
- Keywords:
- Bupleurum chinense DC.; saikosaponin; soil factors; correlation; Chengde area
- 文献标志码:
- A
- 摘要:
- 以承德地区野生和栽培柴胡为试材，采用高效液相色谱法（HPLC）对柴胡根中柴胡皂苷a、柴胡皂苷d含量进行测定，分析了柴胡根际土壤因子与柴胡皂苷含量、柴胡根部颜色的相关性，并采用逐步回归分析方法筛选出影响柴胡药材质量的主要土壤因子,以期为野生柴胡移栽家养、筛选繁育提供参考依据。结果表明:野生移栽柴胡的皂苷含量高于野生柴胡；柴胡皂苷a含量与盐分含量存在显著负相关（P＜0.05）；柴胡皂苷d含量与全磷含量存在极显著正相关（P＜0.01）；柴胡皂苷a+d含量与全磷含量存在显著正相关（P＜0.05）；柴胡根部颜色与铁含量、铵态氮含量、pH存在显著负相关（P＜0.05）；柴胡根部颜色与全磷、土壤类型、全氮、有机质含量存在显著正相关（P＜0.05）；柴胡根部颜色与柴胡皂苷d含量、柴胡皂苷a+d含量存在显著正相关（P＜0.05）。同时，根际土壤因子、土壤类型之间也存在一定相关性，通过逐步回归分析，全磷含量可以解释32.4%的柴胡皂苷d含量信息，盐分含量可以解释19.8%的柴胡皂苷a含量信息，pH和土壤类型可以解释60.6%的柴胡根部颜色信息。柴胡对根际土壤因子养分的丰缺既有适应性，也有选择性。土壤pH和土壤类型是影响柴胡根部颜色的主要因素；而土壤中全磷、盐分的富集促进柴胡皂苷a、柴胡皂苷d的累积。
- Abstract:
- Taking wild and cultivated Bupleurum chinense DC.in Chengde area as the test materials，the contents of saikosaponin a and saikosaponin d in Bupleurum chinense DC.root were determined by high performance liquid chromatography (HPLC).The correlation between rhizosphere soil factors in Bupleurum chinense DC.and saikosaponin content and root color of Bupleurum chinense DC.were analyzed，and the main soil factors affecting the quality of Bupleurum chinense DC.were screened by stepwise regression analysis,in order to provide reference for wild Bupleurum chinense transplanting,domestication screening and breeding.The results showed that the content of saikosaponin in transplanted wild was higher than that in wild Bupleurum chinense DC..There was a extremely significant negative correlation between saikosaponin a content and salt content (P<0.05).There was a significant positive correlation between saikosaponin d content and total phosphorus content (P<0.01).There was a significant positive correlation between saikosaponin a+d content and total phosphorus content (P<0.05).The root color was significantly negatively correlated with iron content，ammonium nitrogen content and pH (P<0.05).There was a significant positive correlation between root color and total phosphorus，soil type，total nitrogen and organic matter content (P<0.05).The root color was positively correlated with the content of saikosaponin d and saikosaponin a+d (P<0.05).At the same time，there was also a certain correlation between rhizosphere soil factors and soil types.Through stepwise regression analysis，total phosphorus content could explain 32.4% of saikosaponin d content information，salt content could explain 19.8% of saikosaponin a content information，and pH and soil types could explain 60.6% of Bupleurum chinense root color information.Bupleurum chinense DC.had both adaptability and selectivity to the abundance and deficiency of rhizosphere soil factors.Soil pH and soil type were the main factors affecting the root color of Bupleurum chinense DC.；the enrichment of total phosphorus and salt in soil promoted the accumulation of saikosaponin a and saikosaponin d.
参考文献/References:
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备注/Memo
第一作者简介：尹鑫（1985-），女，硕士，助理研究员，现主要从事中药资源等研究工作。E-mail：710160369@qq.com.责任作者：张淑彬（1976-），女，硕士，副研究员，现主要从事丛菌种质资源收集保藏及功能评价等研究工作。E-mail：zbinb@163.com.基金项目：〖JP3〗河北省重点研发计划资助项目（20326419D）；河北省重点研发计划项目子课题资助项目（21326312D-4）；承德国家可持续发展议程创新示范区建设科技专项资助项目（202008F016）；承德市科学技术研究与发展计划资助项目（202103B004）；承德市农林科学院科研计划资助项目（202101A005）。收稿日期：2021-09-20