ZHANG Ting,QIAN Liangliang,WANG Weixia,et al.Study on Calcium Enrichment in Mycelia of Hypsizygus marmoreus[J].Northern Horticulture,2022,(04):104-109.[doi:10.11937/bfyy.20213808]
斑玉蕈菌丝体富集钙的研究
- Title:
- Study on Calcium Enrichment in Mycelia of Hypsizygus marmoreus
- Keywords:
- Hypsizygus marmoreus; calcium; mycelia; calcium forms
- 文献标志码:
- A
- 摘要:
- 以斑玉蕈为试材，采用固体和液体培养方法对添加不同浓度氯化钙的菌丝体进行培养，研究了不同培养条件下钙对斑玉蕈菌丝体生长的影响，以及菌丝体对钙的富集与生物转化能力，以期为开发富钙斑玉蕈产品提供参考依据。结果表明：低浓度钙对斑玉蕈菌丝体的生长没有显著影响；固体培养条件下，钙浓度大于250 mg?L-1时抑制菌丝体生长；而在液体培养条件下，钙浓度大于150 mg?L-1时可抑制菌丝体生长。表明斑玉蕈菌丝体在不同培养条件下对钙的耐受性存在较大差异。液体培养条件下，发现随着钙浓度的提高，菌丝体中总钙含量也不断增加。在钙浓度为0~200 mg?L-1时，有机态钙含量比例较高，表明菌丝体在该浓度范围内转化钙能力较强。当钙添加量为100 mg?L-1时，其总钙含量为2 239.8 mg?kg-1，钙富集率为1.83%，其中40.15%为有机态钙。
- Abstract:
- Taking Hypsizygus marmoreus as test material,the mycelia supplemented with different concentrations of calcium chloride were cultured by solid and liquid culture methods,the effects of calcium on the mycelial growth of Hypsizygus marmoreus under solid and liquid culture conditions as well as the enrichment and biotransformation of calcium by mycelia were studied，in order to provide a reference for further development of calcium-rich mushroom products.The results showed that low concentration of calcium had no significant effect on the growth of mycelium of Hypsizygus marmoreus.Under the conditions of solid culture,the growth of mycelium was inhibited when the calcium concentration was greater than 250 mg?L-1.However,the growth of mycelium was inhibited when the calcium concentration was greater than 150 mg?L-1under the conditions of liquid culture.There were great differences in calcium tolerance of Hypsizygus marmoreus mycelium under different culture conditions.At the same time,it was found that the total calcium content in mycelium increased with the increase of calcium concentration under the conditions of liquid culture.In the concentration range of 0-200 mg?L-1,the proportion of organic calcium content was higher,indicated that the mycelium had a strong ability to convert calcium in this concentration range.When the amount of calcium was 100 mg?L-1,the total calcium content was 2 239.8 mg?kg-1,and the calcium enrichment rate was 1.83%,of which 40.15% was organic calcium..
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备注/Memo
第一作者简介：张婷（1987-），女，硕士，助理工程师，现主要从事食用菌工厂化栽培和质量安全控制等研究工作。E-mail:lygzt2013@163.com.责任作者：李福后（1978-），男，博士，副教授，现主要从事食用菌工厂化栽培和设施园艺技术推广等工作。E-mail:lifuhou2002@163.com.基金项目：江苏省政策引导类计划资助项目（LYG-SZ201913）。收稿日期：2021-09-13