GAO Yugang,WEI Xue,ZANG Pu,et al.Isolation and Identification of Germination Fungus Pathogens of Gastrodia elata Bl.Screening and Screen of Antifungal Strains[J].Northern Horticulture,2022,(20):104-111.[doi:10.11937/bfyy.20213681]
天麻萌发菌源病原菌的分离鉴定及其拮抗菌株筛选
- Title:
- Isolation and Identification of Germination Fungus Pathogens of Gastrodia elata Bl.Screening and Screen of Antifungal Strains
- Keywords:
- Gastrodia elata Bl.f.glauca of Changbai Mountain; germination fungus; pathogenic fungus; isolation and identification; antagonistic strains
- 文献标志码:
- A
- 摘要:
- 以染病长白山乌杆天麻萌发菌为试材，通过组织分离法、形态学鉴定法、ITS分子鉴定法及平板对峙法，研究了侵染天麻萌发菌的病原菌种类及其拮抗菌株，以期为长白山乌杆天麻萌发菌的病害防治和种子田间有性繁殖提供参考依据。结果表明：从侵染天麻萌发菌袋的病原菌中分离鉴定出5株真菌，分别为2株哈茨木霉（Trichoderma harzianum），2株深绿木霉（Trichoderma atroviride），1株尖孢镰刀菌（Fusarium oxysporum）；6株植物内生菌均为这5株病原菌的拮抗菌，其中多粘类芽孢杆菌（Paenibacillus polymyxa）拮抗作用最强。
- Abstract:
- Taking the fungi bag of the germination fungus of Gastrodia elata Bl.f.glauca of Changbai Mountain as the experimental material,the species of pathogenic fungus and their antagonistic strains were studied by the method of tissue separation,morphological identification,ITS molecular identification and plate confrontation method,in order to provide reference for the disease control and sexual reproduction of the germination fungus of Gastrodia elata Bl.f.glauca of Changbai Mountain.The results showed that 5 strains of fungi were isolated and identified from the pathogens infecting the germination fungus of Gastrodia elata,including 2 strains of Trichoderma harzianum,2 strains of Trichoderma atroviride,and 1 strain of Fusarium oxysporum.6 plant endophytes were antagonistic to these 5 pathogenic fungus,among which Paenibacillus polymyxa had the strongest antagonistic effect.
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备注/Memo
第一作者简介：郜玉钢（1969-），男，博士，教授，博士生导师，现主要从事生药学等研究工作。E-mail:gaoyugang_2006@163.com.基金项目：吉林省科技发展计划资助项目（20210401093YY,20190301079NY）。收稿日期：2021-09-05