SUN Shijun,MA Bo,WANG Yixuan,et al.Effects of Humic Acid on Soil Nutrients and Microbial Community Structure of Greenhouse Cucumber Rhizosphere Under Salt Stress[J].Northern Horticulture,2022,(05):83-90.[doi:10.11937/bfyy.20213551]
腐殖酸对盐胁迫下温室黄瓜根际土壤养分及微生物群落结构的影响
- Title:
- Effects of Humic Acid on Soil Nutrients and Microbial Community Structure of Greenhouse Cucumber Rhizosphere Under Salt Stress
- Keywords:
- humic acid; cucumber; salt stress; microorganism
- 文献标志码:
- A
- 摘要:
- 以“津优35号”黄瓜为试材，采用盆栽试验法，用150 mmol?L-1 NaCl模拟盐胁迫，通过添加体积比为300、600倍和900倍的腐殖酸进行处理，研究了腐殖酸对盐胁迫土壤养分和微生物群落结构的影响，以期探索腐殖酸对盐胁迫下温室黄瓜土壤微生态环境的作用效果。结果表明：腐殖酸显著提高了土壤中有机质、速效钾、速效磷和全氮含量，而降低了pH。在细菌群落结构方面，各处理间细菌α多样性差异不显著，T1和T2中放线菌门、绿弯菌门和浮霉菌门的相对丰度均高于CK，而拟杆菌门和酸杆菌门的相对丰度则相反，细菌在属水平上组成变化并不明显。在真菌群落结构方面，T1和T2中OTU数量和Shannon指数与CK差异均不显著，而Chao1指数均显著高于CK，腐殖酸还提高了子囊菌门的相对丰度，T1、T2和T3中子囊菌门相对丰度分别较CK高28.07%、26.48%和6.41%，降低了担子菌门和壶菌门的相对丰度，在已知菌属的变化中，毛壳菌属和枝顶孢属的相对丰度升高，根生壶菌属的相对丰度降低。RDA冗余分析表明速效钾是影响细菌群落结构的关键环境因子，而有机质、速效磷和pH则对真菌群落结构起到关键作用。综上，腐殖酸通过改变土壤理化性质及土壤微生物生态系统，对改良土壤盐胁迫具有积极作用。
- Abstract:
- Taking ‘Jinyou No.35’ cucumber as the test material，pot experiment was used,the salt stress was simulated with NaCl concentration of 150 mmol?L-1.The effects of humic acid on soil nutrients and microbial community under salt stress were studied by adding humic acid with volume ratio of 300，600 times and 900 times,in order to explore the effects of humic acid on soil microecological environment of cucumber in greenhouse under salt stress.The results showed that humic acid significantly increased the contents of organic matter，available potassium，available phosphorus and total nitrogen in soil，but decreased the pH.In terms of bacterial community structure，there was no significant difference in bacterial α diversity among treatments.The relative abundances of Actinobacteria，Chloroflexi and Planctomycetes in T1 and T2 were higher than CK，while the relative abundances of Bacteroidetes and Acidobacteria were opposite.The changes of bacterial composition at the genus level was not obvious.In terms of fungal community structure，the OTU number and Shannon index of fungi in T1 and T2 were not significantly with CK，while the Chao1 index was significantly higher than CK.Humic acid also significantly increased the relative abundance of Ascomycota in T1，T2 and T3，which were 28.07%，26.48% and 6.41% higher than CK，and reduced the relative abundance of Basidiomycota and Chytridiomycota.Among the changes of known genera，the relative abundance of Chaetomium and Acremonium increased，and the relative abundance of Rhizophydium decreased.RDA redundancy analysis showed that available potassium was the key environmental factor affecting bacterial community structure，while organic matter，available phosphorus and pH were the key factors affecting fungal community structure.In conclusion，humic acid had a positive effect on improving salt-stressed soil by changing the physical and chemical properties of the soil and the soil microbial ecosystem.
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备注/Memo
第一作者简介：孙世君(1988-)，男，博士，讲师，现主要从事设施蔬菜高效栽培及抗性生理等研究工作。E-mail：1048197709@qq.com.责任作者：郝水源（1975-），男，博士，副教授，现主要从事设施蔬菜高效栽培技术等研究工作。E-mail：Haoshuiyuan@126.com.基金项目：内蒙古自治区自然科学基金资助项目(2021BS03009)；内蒙古自治区科技计划资助项目(19041201011)。收稿日期：2021-08-28