WANG Yue,REN Ziming,YU Yanshen.Identificaton of Morchella esculenta and Growth Conditions Optimization[J].Northern Horticulture,2022,(06):114-121.[doi:10.11937/bfyy.20213188]
羊肚菌菌种鉴定及生长条件优化
- Title:
- Identificaton of Morchella esculenta and Growth Conditions Optimization
- 文献标志码:
- A
- 摘要:
- 以12株羊肚菌菌株为试材，采用拮抗试验和分子鉴定技术，研究了供试菌株的亲缘关系及不同碳、氮源、pH对菌丝生长情况的影响，以期规范羊肚菌命名及初步筛选最佳生长条件。结果表明：YD3、YD10、YD12亲缘关系较近，YD1、YD2、YD4、YD5、YD7、YD8、YD9、YD11亲缘关系较近，YD6与其它11个菌株亲缘关系较远；最佳碳源是乳糖和麦芽糖，最佳氮源是硫酸铵和硝酸钾，最适pH为7和9。
- Abstract:
- The 12 isolates of Morchella esculenta were used as experimental materials，using antagonistic test and molecular identification technique，the genetic relationship of the tested strains and the effects of different carbon,nitrogen sources and pH on mycelial growth were studied,in order to solve the naming confusion problem of Morchella esculenta and obtain the optimum growth conditions for the initial screening.The results showed that YD3、YD10、YD12 had closer relationships than others，YD1、YD2、YD4、YD5、YD7、YD8、YD9、YD11 had closer relationships than others,YD6 had distant relationships with other 11 strains.Lactose and maltose were the best carbon sources.(NH4)2SO4 and KNO3 were the best nitrogen sources.The optimum growth pH were 7 and 9.
参考文献/References:
[1]贺新生.羊肚菌生物学基础、菌种分离制作与高产栽培技术[M].北京:科学出版社,2017.[2]黄年来,林志彬,陈国良,等.中国食药用菌学[M].上海:上海科学技术文献出版社,2010.[3]杜习慧,赵琪,杨祝良.羊肚菌的多样性、演化历史及栽培研究进展[J].菌物学报,2014,33(2):183-197.[4]谭方河.羊肚菌人工栽培技术的历史、现状及前景[J].食药用菌,2016,24(3):140-144.[5]OWER R.Notes on the development of the morel ascocarp:Morchella esculenta[J].Mycologia,1982,74(1):142-144.[6]贺国强,魏金康,邓德江,等.北京地区人工栽培羊肚菌的鉴定[J].食用菌,2018,40(2):29-31.[7]何培新,刘伟,蔡英丽,等.我国人工栽培和野生黑色羊肚菌的菌种鉴定及系统发育分析[J].郑州轻工业学院学报(自然科学版),2015,30(Z1):26-29.[8]赵瑞,刘绍雄,马超.我国羊肚菌产业发展现状及市场分析[J].中国食用菌,2020,39(2):7-10.[9]赵琪,黄韵婷,徐中志,等.羊肚菌栽培研究现状[J].云南农业大学学报,2009,24(6):904-907.[10]赵琪,康平德,戚淑威,等.羊肚菌资源现状及可持续利用对策[J].西南农业学报,2010,23(1):266-269.[11]HE P X.Effect of aging on culture and cultivation of the culinary-medicinal mushrooms Morchella importuna and M.sextelata (Ascomycetes)[J].International Journal of Medicinal Mushrooms,2019,21(11):1089-1098.[12]贺立虎,李娟丽,王文光.羊肚菌研究与利用现状[J].陕西农业科学,2021,67(5):93-96.[13]WORRALL J J.Somatic incompatibility in basidiomycetes[J].Mycologia,1997,89(1):24-36.[14]马瑜,申坚定,宋君锋,等.羊肚菌不同栽培品种间拮抗试验反应[J].中国食用菌,2017,36(5):80-82.[15]王磊,蔡德华,宿红艳,等.拮抗实验和RADP对鸡腿菇栽培菌株亲缘关系的研究[J].安徽农业科学,2006(16):3903-3904.[16]李青,韩鹏远,柴美清,等.不同碳源对3种羊肚菌菌丝生长的影响[J].农学学报,2018,8(7):42-45.[17]龚燕川,陈影,唐杰,等.梯棱羊肚菌M83和M101菌株培养特性差异[J].中国食用菌,2016,35(4):7-11.[18]王泽清,徐中志.粗腿羊肚菌菌丝栽培条件研究[J].西南农业学报,2010,23(1):149-152.[19]熊春菊,赵琪,徐中志,等.尖顶羊肚菌菌丝培养研究[J].现代农业科技,2009(8):13-14.[20]张保顺,叶孟婷,刘清,等.黑脉羊肚菌固体培养基的制备及其培养条件优化[J].西南师范大学学报(自然科学版),2020,45(6):52-62.[21]董爱荣,吴庆禹,何力,等.羊肚菌的生物学特性[J].东北林业大学学报,2002(4):28-30.[22]张松,夏艳红,姚珍平.羊肚菌菌丝生物学特性研究[J].食用菌学报,2002(1):18-21.[23]朱永真,杜双田,车进,等.不同碳源及氮源对羊肚菌菌丝生长的影响[J].西北农林科技大学学报(自然科学版),2011,39(3):113-118.[24]周洁,谭伟,曹雪莲,等.羊肚菌A1菌株菌丝体的培养特性[J].中国食用菌,2016,35(4):12-17.[25]谢放,吴萍民,赵春巧.7株羊肚菌菌丝的生物学特性研究[J].中国农学通报,2014,30(10):140-147.[26]陈慧群,刘洪玉.尖顶羊肚菌生物学特性的研究[J].食用菌,1995(5):18-19.[27]王秀云.尖顶羊肚菌生物学特性的研究[J].食用菌,1998(1):13-14.[28]陶热,谭玉琴,陈晔,等.小羊肚菌菌丝培养条件的研究[J].北方园艺,2011(17):183-187.[29]陈易飞,郭益红.温度、pH对羊肚菌菌丝体生长的影响[J].苏南科技开发,2007(8):28-29.
相似文献/References:
[1]权美平,张丽芳.羊肚菌生物学特征及价值的研究进展[J].北方园艺,2012,36(18):178.
QUAN Mei-ping,ZHANG Li-fang.Research Advances on the Ecological Niche and Value of Wild Morchella[J].Northern Horticulture,2012,36(06):178.
[2]武冬梅,许文涛,谢宗铭,等.新疆伊犁昭苏野生羊肚菌分离株的分子鉴定研究[J].北方园艺,2014,38(21):145.
WU Dong-mei,XU Wen-tao,XIE Zong-ming,et al.Molecular Identification of Morchella in Xinjiang Yili[J].Northern Horticulture,2014,38(06):145.
[3]王 广 耀,董 立 华,张红.液体羊肚菌浅层发酵培养基的优化研究[J].北方园艺,2010,34(07):0.[doi:10.11937/bfyy.201007068]
W ANG Guang -yao,DONG L- i hua,ZHANG Hong.The Study of O ptimization Conditions about Fermentation Medium Shallow of Morchella[J].Northern Horticulture,2010,34(06):0.[doi:10.11937/bfyy.201007068]
[4]陈 国 梁,张 向 前,贺 晓 龙,等.五种羊肚菌液体培养过程胞外酶活性变化研究[J].北方园艺,2010,34(10):0.[doi:10.11937/bfyy.2010010077]
CHEN Guo -liang,ZHANG Xiang -qian,HE Xiao -long,et al.Study on Active Charge of Various Exocellular Enzeyme fromFive Morchella esculentas in Liquid Culture Medium[J].Northern Horticulture,2010,34(06):0.[doi:10.11937/bfyy.2010010077]
[5]胥芮,刘玉萍,张晓宇,等.青海互助北山林场羊肚菌营养成分初探[J].北方园艺,2016,40(10):138.[doi:10.11937/bfyy.201610035]
XU Rui,LIU Yuping,ZHANG Xiaoyu,et al.Study on Nutritional Components of Morchella vulgaris From Beishan Forest in Huzhu of Qinghai Province[J].Northern Horticulture,2016,40(06):138.[doi:10.11937/bfyy.201610035]
[6]张晓宇,周勇辉,吕婷,等.青海互助北山林场羊肚菌菌核培养条件的优化[J].北方园艺,2016,40(18):146.[doi:10.11937/bfyy.201618036]
ZHANG Xiaoyu,ZHOU Yonghui,LYU Ting,et al.Optimization of Culture Conditions for Morchella vulgaris Sclerotium From Huzhu′ Beishan Forest in Qinghai Province[J].Northern Horticulture,2016,40(06):146.[doi:10.11937/bfyy.201618036]
[7]吴 韶 菊.羊肚菌固体培养基的筛选[J].北方园艺,2009,33(03):0.[doi:10.11937/bfyy.200903084]
WU Shao -ju.Screening of Morchellacrassipes Solid Medium[J].Northern Horticulture,2009,33(06):0.[doi:10.11937/bfyy.200903084]
[8]王 广 耀,蒋 晓 成,程 喆.羊肚菌菌种分离及母种培养基的筛选[J].北方园艺,2009,33(09):0.[doi:10.11937/bfyy.200909080]
W ANG Guang -yao,JIANG Xiao -cheng,CHENG Zhe.Strains Separated and Selection of Mother Culture Media of Morchella[J].Northern Horticulture,2009,33(06):0.[doi:10.11937/bfyy.200909080]
[9]张晓宇,吕婷,刘涛,等.青海互助北山林场羊肚菌菌丝体培养条件优化[J].北方园艺,2017,41(08):144.[doi:10.11937/bfyy.201708033]
ZHANG Xiaoyu,LYU Ting,LIU Tao,et al.Culture Conditions Optimization for Morchella vulgaris Mycelium From Huzhu Beishan Forest in Qinghai Province[J].Northern Horticulture,2017,41(06):144.[doi:10.11937/bfyy.201708033]
[10]韩融冰,赵玉卉,路等学,等.羊肚菌多糖提取工艺研究[J].北方园艺,2018,42(23):150.[doi:10.11937/bfyy.20181499]
HAN Rongbing,ZHAO Yuhui,LU Dengxue,et al.Study on Extraction Process of Morchella Polysaccharides[J].Northern Horticulture,2018,42(06):150.[doi:10.11937/bfyy.20181499]
备注/Memo
第一作者简介：王月（1988-），女，硕士，助理研究员，现主要从事食用菌栽培育种等研究工作。E-mail：wy13844428953@163.com.责任作者：于延申（1965-），男，硕士，研究员，现主要从事食用菌栽培育种等研究工作。E-mail：syj20210803@163.com.基金项目：吉林省省级部门预算科学研究资助项目（20201103）。收稿日期：2021-08-03