LI Jianmei,CHI Jingliang,XIE Cunyi,et al.Study on Culture Conditions of Lnonotus linteu S-1[J].Northern Horticulture,2021,(23):127-133.[doi:10.11937/bfyy.20212454]
桑黄菌株S-1培养条件研究
- Title:
- Study on Culture Conditions of Lnonotus linteu S-1
- Keywords:
- Lnonotus linteu; culture condition; artificial cultivation
- 文献标志码:
- A
- 摘要:
- 以桑黄菌株（Inonotus linteu）S-1为试材，采用人工栽培试验,研究了不同培养基、培养温度、初始pH、桑叶提取物浓度对菌丝生长特性的影响，以期为桑黄菌株S-1人工栽培及开发应用提供参考依据。结果表明：桑黄菌株S-1最适斜面培养基为PDA，菌丝生长最适温度25 ℃、最适pH为50~55；优化条件下，在培养基中添加005%~030%的桑叶提取物，对试验菌株菌丝生长具有明显促进作用，其中以添加浓度为025%时促生长作用最为明显；在人工栽培培养基上，桑黄菌株S-1具有良好的子实体形成能力，其最适栽培培养基为木屑78％、麸皮18％、豆饼粉2％、磷酸二氢钾02％、硫酸镁01％、蔗糖02％，生石灰1％、石膏粉1％、桑叶提取物025%，含水量65％。
- Abstract:
- Lnonotus linteu strain S-1 was taken as the test material.Factors that affected the growing characteristics of Lnonotus linteu strain S-1 mycelia were investigated,including cultivation medium,pH,temperature,and concentration of mulberry leaf extract in order to provide reference for the artificial cultivation and development ofapplication of Lnonotus linteu S-1.The results showed that the optimum cultivation medium was PDA.The optimum pH was 50-55,and the optimum growth temperature was around 25 ℃005%-030% addition of mulberry leaf extract to the culture medium obviously promoted the mycelial growth of the tested strain,and the optimum concentration of mulberry leaf extract was 025%.Lnonotus linteu strain S-1 had a good capacity to form fruiting body.The optimized artificial cultivation medium consisted of sawdust 78%,bran 18%,bean cake powder 2%,KH2PO4 02%,MgSO4 01%,sucrose 02%,lime 1%,gypsum 1%,mulberry leaf extract 025%,and moisture content 65%.
参考文献/References:
[1]梁晓薇,刘远超,黄龙花,等.桑黄孔菌属的研究进展[J].微生物学杂志,2017,37(6):98-104.[2]陈万超,杨簸,张劲松,等.桑黄类真菌活性代谢产物的研究进展[J].食用菌学报,2020,27(4):188-201.[3]戴玉成,崔宝凯.药用真菌桑黄种类研究[J].北京林业大学学报,2014,36(5):1-6.[4]宋吉玲,王伟科,程俊文,等.野生桑黄的分离鉴定及培养特性研究[J].西北农林科技大学学报(自然科学版),2020,48(3):130-135.[5]张维博,王家国,李正阔,等.药用真菌桑黄的研究进展[J].中国中药杂志,2014,39(15):2838-2845.[6]吕英华,王建芳,李玉平,等.药用真菌桑黄的研究进展[J].蚕业科学,2009(1):204-211．[7]IKEKAWA T,NAKANISHIM,UEHARAN,et al.Antitumor action of some Basidiomycetes,especially Phllinus linteus[J].Gan,1968(2):155-157.[8]曲名,王波,李玉.桑黄菌种生长条件的研究[J].西南农业学报,2013,26(5):2173-2175.[9]雷萍,孙悦迎,张文隽,等.野生桑黄菌种分离与培养特性研究初报[J].食用菌学报,2007,14(2):71-75.[10]姜明,刘岩,王伟功,等.不同pH值和培养基对桑黄茵丝生长的影响[J].北方园艺,2010(8):192-194.[11]孙波,周洪英,吴洪丽,等.湖北野生桑黄分离鉴定及培养研究[J].湖北农业科学,2016,55(12):1-2.[12]姜福春,冯杰,张赫男,等.桑树水提物对桑黄液态深层发酵的影响[J].上海农业学报,2017,33(1):56-62.[13]施阳阳,蒲彬,刘燕林,等.桑枝屑不同添加量对桑黄的生物转化率和质量的影响[J].蚕学通讯,2019,39(2):1-4.
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备注/Memo
第一作者简介：李剑梅(1967-)，女，本科，研究员，现主要从事珍稀食药用菌研发等研究工作。E-mail:84386120@qq.com.责任作者：池景良（1964-），男，本科，研究员，现主要从事珍稀食药用菌研发等研究工作。E-mail:chijingliang@sina.com.基金项目：辽宁省兴辽英才计划资助项目（XLYC2002048）。收稿日期：2021-06-11