CHEN Shaoxian,LIU Yue,ZENG Yaoying,et al.Isolation and Identification of Pathogenic Bacteria of Olecranon Peach After Harvest[J].Northern Horticulture,2021,(17):108-118.[doi:10.11937/bfyy.20210293]
鹰嘴桃采后病原菌的分离与鉴定
- Title:
- Isolation and Identification of Pathogenic Bacteria of Olecranon Peach After Harvest
- 文献标志码:
- A
- 摘要:
- 以鹰嘴桃采后病果为试材，用组织分离法对腐败鹰嘴桃中的病原菌进行分离纯化，通过致病能力检测筛选得到优势病原菌。采用形态学鉴定、生理生化试验和分子生物学技术对优势病原菌进行菌种鉴定，以期为今后鹰嘴桃病害的深入研究提供参考依据。结果表明：从鹰嘴桃病果中共分离得到23株病原菌，其中有优势病原菌11株。优势病原菌株种类为贝莱斯芽孢杆菌（Bacillus velezensis）、解淀粉芽孢杆菌（Bacillus amyloliquefaciens）、日本葡萄球菌（Gluconobacter japonicus）、乳酸乳球菌（Lactococcus lactis）、黑曲霉（Aspergillus niger）、哈茨木霉（Trichoderma harzianum）、胶孢炭疽菌（Colletotrichum gloeosporioides）和白色念珠菌（Geotrichum candidum）。其中菌株贝莱斯芽孢杆菌zk1的致病能力最强且潜在宿主较多。该研究首次发现贝莱斯芽孢杆菌是桃类果实的病原菌。
- Abstract:
- The diseased fruit of olecranon peach after harvest were used as experimental materials,in which the pathogenic bacteria were separated and purified.Predominant pathogens were screened by pathogenicity.Morphological observation,physiological and biochemical analyses,and molecular methods were performed to characterize predominant pathogens,in order to provide reference for indepth research on olecranon peach disease in the future.The results showed that 23 pathogens were isolated from the rotten olecranon peach,among which 11 were the predominant pathogen including Bacillus velezensis,Bacillus amyloliquefaciens,Gluconobacter japonicus,Lactococcus lactis,Aspergillus niger,Trichoderma harzianum,Colletotrichum gloeosporioides,and Geotrichum candidum.Among them,Bacillus velezensis zk1 had the strongest pathogenic ability and more potential hosts.In this study,Bacillus velezensis was fisrt found to be the pathogen of peach.
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备注/Memo
第一作者简介：陈少先（1996-），女，硕士研究生，研究方向为微生物与水果贮藏。E-mail:2312625562@qq.com.责任作者：余倩（1979-），女，博士，教授，现主要从事微生物与果蔬贮藏的教学与科研等工作。E-mail:yuqianchina@126.com.基金项目：广州市科技厅资助项目（201704020191）;仲恺农业工程学院研究生处资助项目（KJCX2021006）。收稿日期：2021-01-19