YUE Jianhua,DONG Yan,LU Youfa,et al.Effects of pH on Physiological Characters of Agapanthus praecox Cell Proliferation in vitro[J].Northern Horticulture,2021,(15):73-80.[doi:10.11937/bfyy.20210282]
pH调节百子莲离体细胞增殖的生理特性
- Title:
- Effects of pH on Physiological Characters of Agapanthus praecox Cell Proliferation in vitro
- Keywords:
- pH; cell proliferation; plant hormone; sugar metabolism; stress
- 文献标志码:
- A
- 摘要:
- 以百子莲小花梗为外植体诱导的愈伤组织（Ca）、胚性愈伤组织（EC）为试材，测定不同pH处理Ca、EC内源激素、糖、活性氧及保护酶差异，研究梯度pH（5.6、5.8、6.0、6.2）对Ca、EC增殖的影响，以期揭示培养基pH对百子莲离体细胞增殖生理特性的影响，为再生体系优化提供参考依据。结果表明：Ca、EC继代阶段较佳pH为5.8，Ca增殖阶段，pH影响GA4、麦芽糖和果糖的积累，而GA4含量、麦芽糖和果糖与细胞团直径显著正相关，Pearson相关系数分别为0.955、0.999和0.900。EC增殖阶段，结合态IAA、GA4、总糖、·OH含量与EC细胞团直径显著正相关，相关系数分别为0.985、0.920、0.931、0.911。pH可通过调节离体细胞激素代谢、糖代谢、胁迫信号影响百子莲细胞增殖效果。
- Abstract:
- Pedicel originated callus (Ca) and embryogenic callus (EC) were used as materials,endogenous hormone metabolism,sugar metabolism,reactive oxygen species and protective enzymes with gradient pH treatment were detected.The effects of gradient medium pH (5.6,5.8,6.0 and 6.2) on Ca,EC cell proliferation was studied,in order to investigate the effects of medium pH on the physiological characteristics of cell proliferation in vitro and provide reference for regeneration system optimization in Agapanthus praecox.The results showed that the optimal pH in Ca and EC cell proliferation was 5.8.At the stage of Ca proliferation,pH affected cell proliferation by regulating GA4,maltose and fructose accumulation.Subsequently,GA4,maltose and fructosewere significantly positively correlated with cell mass diameter,with correlation coefficients of 0.955,0.999 and 0.900,respectively.At the stage of EC proliferation,the contents of binding IAA,GA4,total saccharides and ·OH were positively correlated with the EC cell mass diameter,with correlation coefficients of 0.985,0.920,0.931 and 0.911,respectively.pH effects cell proliferation in vitro by regulating hormone metabolism,sugar metabolism and stress signal in Agapanthus praecox.
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备注/Memo
第一作者简介：岳建华（1982-），男，博士，副教授，现主要从事园艺植物发育生物学等研究工作。E-mail：jhyues@163.com.责任作者：龚守富（1976-），男，硕士，副教授，现主要从事园艺植物种质资源等研究工作。E-mail：hortiplants@163.com.基金项目：国家自然科学基金资助项目（31670693）；信阳农林学院高水平科研孵化器建设基金资助项目（FCL202012）；信阳农林学院科技创新团队资助项目（CXTD-202002）；信阳农林学院园艺专业综合改革试点建设资助项目（ZYZHGG201803）。收稿日期：2021-01-19