ZHANG Wenbo,WANG Yuejun.Root Exudates of Stipa Community in Desert Steppe Regulate the Structure of Soil Microbial Community[J].Northern Horticulture,2021,(22):104-111.[doi:10.11937/bfyy.20205520]
荒漠草原针茅群落根系分泌物对土壤微生物群落结构的影响
- Title:
- Root Exudates of Stipa Community in Desert Steppe Regulate the Structure of Soil Microbial Community
- Keywords:
- root exudates; soil nutrients; soil microorganism
- 文献标志码:
- A
- 摘要:
- 以短花针茅为试材，提取根系分泌物，鉴定根系分泌物的组分，探究不同浓度根系分泌物（C0，C1，C2，C3）对土壤养分、酶活性、微生物量、土壤微生物群落功能多样性、微生物代谢熵的影响，以期了解荒漠草原针茅群落根系分泌物调控土壤微生物群落机制。结果表明：在短花针茅根系分泌物中发现21种组分，其中根系分泌物酯类物质含量最高，包括烃类8种，酯类10种，苯酚类2种，酮类1种；四十四苯烷（13.59％）、顺-9-十八碳烯酸-甲基酯（16.45％）、乙基-5-十八烷（21.78％）、4，6-二甲基十二烷（12.78％）、1，2-二甲苯（10.34％）占根系分泌物总量的74.94％。C1、C2、C3处理下全氮、有机碳和全钾均显著高于CK（P<0.05），其大小依次表现为C2>C3>C1>CK，C3和C1差异不显著（P>0.05）。土壤蔗糖酶活性、过氧化氢酶、脲酶、微生物量碳、氮大小依次表现为C2>C3>C1>CK，C3和C1差异不显著（P>0.05）。C1、C2、C3处理下土壤微生物均匀度指数、物种丰富度指数、碳源利用强度大小依次表现为C2>C3>C1>CK，C3和C1差异不显著（P>0.05）。相关性分析表明，微生物群落代谢特征在C2处理与CK相似，与C3处理具有显著差异，说明不同浓度根系分泌物调控土壤微生物群落结构机制不一致。综上所述，低浓度的根系分泌物对土壤酶活性、微生物群落及代谢熵具有一定的促进作用，而高浓度的表现为一定的抑制作用。
- Abstract:
- Taking Stipa breviflora as test material,the root secretion of components was identified，and then plant roots secretion solution was added to the soil，the effects of the different concentrations of exogenous root secretion (C0，C1，C2 and C3) on soil nutrients，enzyme activities and microbial biomass，soil microbial community functional diversity，the microbial metabolism entropy were studied,in order to explore the desert grassland of S.grandis community root secretion regulation mechanism of soil microbial community.The results showed that 21 species of root exudates were found in Stipa stipa,among which the esters were the highest,including 8 kinds of hydrocarbons,10 kinds of esters,2 kinds of phenol and 1 kind of ketone.Forty-fourbenzene alkyl (13.59%),cis-9-octadecenoic acid-methyl ester (16.45%),ethyl-5-octadecane (21.78%),4,6-dimethyldodecane (12.78%),1,2-xylene (10.34%) accounted for 74.94% of the total root exudates,desert grassland was short fancy stitch couchgrass root system secretion of main ingredients.The contents of organic carbon，total nitrogen and total potassium in root exudates with different concentrations were significantly higher than those of CK (P<0.05) which were in turn as follow,C2>C3>C1>CK，C1 and C3 were not significantly different (P>0.05).The activities of sucrase，catalase，urease，microbial biomass carbon and nitrogen content in soil treated with root exudates of different concentrations were significantly higher than those of CK，which were in turn as follows,C2>C3>C1>CK，C1 and C3 showed no significant difference (P>0.05).Soil microbial species richness index，evenness index and carbon source utilization richness index were significantly higher than those of CK under different concentrations of plant root exudates (P<0.05)，which were in turn as follows,C2>C3>C1>CK，with no significant difference between C3 and C1 (P>0.05).Redundancy analysis showed that the soil treated with medium concentration root exudates had similar metabolic characteristics to the soil treated with high concentration root exudates，which was significantly different from that treated with high concentration root exudates，indicating that they had different utilization ability of a single carbon source and their community metabolic characteristics were different.In general，the effects of root exudates on soil nutrients，enzyme activity，microbial biomass，functional diversity of soil microbial community，and microbial metabolism entropy showed a concentration effect of low promotion and high inhibition.
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备注/Memo
第一作者简介：张文博（1984-），女，硕士，讲师，现主要从事环境科学与工程等研究工作。E-mail:Wenbo_zhang84@126.com.收稿日期：2020-12-24