ZHAN Lifeng,LI Chunxin,HE Kai,et al.Effects of Overexpression of [STBX]MsRCI2A,MsRCI2B and MsRCI2C[STBZ] Genes on Cold Tolerance of Alfalfa[J].Northern Horticulture,2021,(18):67-74.[doi:10.11937/bfyy.20205515]
[STBX]MsRCI2A、MsRCI2B、MsRCI2C[STBZ]基因超量表达对紫花苜蓿耐冷的影响
- Title:
- Effects of Overexpression of [STBX]MsRCI2A,MsRCI2B and MsRCI2C[STBZ] Genes on Cold Tolerance of Alfalfa
- 关键词:
- 紫花苜蓿; 冷胁迫; [STBX]MsRCI2[STBZ]类基因; 转基因苜蓿
- 文献标志码:
- A
- 摘要:
- 以紫花苜蓿为试材，对紫花苜蓿来源的[STBX]RCI2[STBZ]类基因[STBX]MsRCI2A/2B/2C[STBZ]在4 ℃的表达差异进行分析，采用农杆菌介导的方法对紫花苜蓿进行遗传转化，研究了超量表达[STBX]MsRCI2A/2B/2C[STBZ]基因对紫花苜耐冷性的影响，以期为培育耐冷紫花苜蓿提供参考依据。结果表明：[STBX]MsRCI2A/2B/2C[STBZ]基因均受低温诱导，转基因苜蓿材料经4 ℃冷处理12 d后，叶绿素相对含量、相对电导率、丙二醛含量才发生明显变化，对冷的敏感性下降。并且4 ℃处理后，转基因株系较WT（野生型）积累更多脯氨酸和可溶性糖，抗氧化酶（SOD、POD和CAT）活性在低温6 d时迅速上升，响应冷胁迫更迅速。综上所述，[STBX]MsRCI2A/2B/2C[STBZ]基因的超量表达提高了紫花苜蓿的耐冷性。
- Abstract:
- Alfalfa was used as the test material.The expression differences of [STBX]RCI2 genes MsRCI2A/2B/2C[STBZ] derived from alfalfa were analyzed at 4 ℃,and then the genetic transformation of alfalfa was carried out by agrobacterium-mediated method.The effect of overexpression of [STBX]MsRCI2A/2B/2C[STBZ] gene on cold tolerance of alfalfa,in order to provide reference for cultivating cold tolerant alfalfa.The results showed that the [STBX]MsRCI2A/2B/2C[STBZ] genes were all induced by 4 ℃.The chlorophyll relative content,relative conductivity,and MDA content of the transgenic alfalfa changed significantly after 12 days of 4 ℃ treatment,indicating that their sensitivity to 4 ℃ was reduced.And after treatment at 4 ℃,the transgenic plants accumulated more proline and soluble sugar than WT plants,and the activities of antioxidant enzymes (SOD,POD and CAT) increased rapidly at 6 days of treatment at 4 ℃,and responded to cold stress more rapidly.In summary,the overexpression of [STBX]MsRCI2A/2B/2C[STBZ] genes improved the cold tolerance of alfalfa.
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备注/Memo
第一作者简介：战力峰（1995-），男，硕士研究生，研究方向为植物基因工程。E-mail：1048201547@qq.com.责任作者：才华（1979-），女，博士，副教授，硕士生导师，现主要从事牧草耐碱分子生物学及基因工程等研究工作。E-mail：caihuaneau@gmail.com.基金项目：国家自然科学基金面上资助项目（32071866）。收稿日期：2020-12-24