WANG Yanfang,LI Fayuan,LI Lingzhi,et al.Effects of Different Combinations of CaCl2 Reagents on Low-temperature Germination of Summer Squash Seeds[J].Northern Horticulture,2021,(15):40-45.[doi:10.11937/bfyy.20204868]
不同CaCl2试剂组合对西葫芦种子低温萌发的影响
- Title:
- Effects of Different Combinations of CaCl2 Reagents on Low-temperature Germination of Summer Squash Seeds
- Keywords:
- combination of CaCl2 reagents; summer squash seeds (Cucurbita pepo L.); low temperature; germination
- 文献标志码:
- A
- 摘要:
- 以‘春翡翠’西葫芦种子为试材，采用CaCl2溶液、CaCl2 + 脲基二琥珀酸四钠（HIDS）溶液、CaCl2 + 黄腐酸（FA）溶液、去离子水（CK）浸种，研究不同CaCl2试剂组合浸种对低温下种子萌发的影响，以期为提高西葫芦抗冷性提供参考依据。结果表明：与对照相比，添加CaCl2的3个处理均可提高西葫芦种子在低温下的萌发能力，表现为CaCl2+FA>CaCl2+HIDS>CaCl2>CK。与对照相比，CaCl2+FA浸种效果最好，浸种后西葫芦种子发芽率、发芽势、发芽指数、活力指数显著提高2.48%、2.48%、1.15%、14.71%；根长、鲜质量、干质量显著增加15.12%、46.47%、13.35%；CaCl2+FA溶液浸种提高了芽苗中渗透调节物质含量及抗氧化酶活性，使芽苗中脯氨酸、可溶性糖、可溶性蛋白质含量、超氧化物歧化酶、过氧化物酶活性分别增加14.52%、180.00%、31.78%、1.52%、53.53%；超氧阴离子自由基、过氧化氢含量分别降低了131.97%、35.83%。综上所述，不同CaCl2试剂组合均可减轻低温对种子萌发的伤害，其中CaCl2与FA组合浸种缓解低温伤害效果最佳。
- Abstract:
- ‘Spring Jadeite’ summer squash (Cucurbita pepo L.) seeds were used as experimental materials,the seeds were soaked with CaCl2 solution, CaCl2 + Urea disuccinate tetrasodium (HIDS) solution, CaCl2 + Fulvic acid solution (FA) solution and deionized water (CK) to study the effects of different CaCl2 reagent combination on seed germination at low temperature,in order to provide reference for improving the cold resistance of summer squash.The results showed that compared with the control,the three treatments with CaCl2 could improve the germination ability of summer squash seeds under low temperature,which was CaCl2+FA>CaCl2+HIDS>CaCl2>CK.Compared with the control,the germination rate,germination potential,germination index and vigor index of summer squash seeds soaked in CaCl2+FA solution increased significantly by 2.48%,2.48%,1.15% and 14.71%,while the root length,fresh weight and dry weight increased by 15.12%,46.47% and 13.35%;soaking seeds in CaCl2+FA solution enhanced osmotic adjustment substances and antioxidant enzyme activity,and increased proline,soluble sugar,soluble protein contents,SOD,POD activities by 14.52%,180.00%,31.87%,1.52%,53.53%,respectively.The contents of superoxide anion radica and H2O2 decreased by 131.97% and 35.83% respectively.In conclusion,different combinations of CaCl2 reagents could reduce the damage of low temperature on seed germination,and the combination of CaCl2 and FA had the best effect。
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备注/Memo
第一作者简介：王艳芳（1982-），女，硕士，高级实验师，现主要从事园艺植物生理生态等研究工作。E-mail：wyfwbbwy@163.com.责任作者：李灵芝（1972-），女，博士，教授，现主要从事蔬菜栽培生理和设施园艺等研究工作。E-mail：lingzhili@sxau.edu.cn.基金项目：国家农业科技成果转化资金资助项目（2014GB2A300017）；山西省科技攻关资助项目（20110311016-2）。收稿日期：2020-11-18