QI Ronglian,Sharentuya,LI Gangtie,et al.Seed Germination Characteristics of Halimodendron halodendron (Pall.)Voss Under Salt and Drought Stress[J].Northern Horticulture,2021,(15):81-88.[doi:10.11937/bfyy.20204305]
盐旱胁迫下铃铛刺种子萌发特性
- Title:
- Seed Germination Characteristics of Halimodendron halodendron (Pall.)Voss Under Salt and Drought Stress
- 文献标志码:
- A
- 摘要:
- 以呼和浩特地区引种的铃铛刺种子为试材，采用不同浓度的NaCl溶液（4、8、12、16、20 g·L-1）和PEG-6000溶液（5%、10%、15%、20%、25%）模拟盐旱胁迫方法，研究盐旱胁迫下铃铛刺种子的萌发特性，以期为铃铛刺在干旱半干旱地区用于改良盐碱土及生态修复提供参考依据。结果表明：随盐胁迫程度的增大，铃铛刺种子的萌发率、萌发势呈先上升后下降的趋势，萌发指数、活力指数、胚根胚芽长随NaCl浓度的升高呈下降趋势；干旱胁迫下，各指标均随PEG-6000溶液浓度的升高而降低，低浓度（5%）对种子萌发抑制作用不明显；NaCl溶液和PEG-6000溶液浓度分别为20 g·L-1和25%时，铃铛刺种子的萌发率为54.67%和25.33%，未达到完全抑制其萌发的盐旱阈值，经引种的铃铛刺种子在萌发期仍具有较高的耐盐、耐旱特性。
- Abstract:
- Halimodendron halodendron (Pall.) Voss seed introduced in Hohhot was used as the research material.Different concentrations of NaCl solution (4 g·L-1,8 g·L-1,12 g·L-1,16 g·L-1,20 g·L-1) and PEG-6000 solution (5%,10%,15%,20%,25%) were set to simulate the salt and drought stress to explore the germination characteristics of the Halimodendron halodendron (Pall.) Voss seed under salt and drought stress,in order to provide a reference for Halimodendron halodendron (Pall.) Voss to improve saline-alkali soil and ecological restoration in arid and semi-arid areas.The results showed that,with the increase of salt stress degree,the seed germination rate and germination potential of Halimodendron halodendron (Pall.) Voss showed a trend of first increased and then decreased;the germination index,vigor index,length of radicle and plumule decreased with the increase of NaCl concentration.Under drought stress,all indexes decreased with the increase of PEG-6000 solution concentration,and low concentration (5%) of PEG-6000 solution had no significant inhibitory effect on seed germination.When the concentration of NaCl and PEG-6000 solutions were 20 g·L-1 and 25%,the seed germination rate of Halimodendron halodendron (Pall.) Voss was 54.67% and 25.33% which did not reach the threshold of salt and drought for germination.
参考文献/References:
[1]户连荣,郎南军,郑科.植物抗旱性研究进展及发展趋势[J].安徽农业科学,2008,36(7):2652-2654.[2]李佳,何丽君,陈海军,等.黑果枸杞(Lycium ruthenium)种子萌发特性与抗旱性[J].分子植物育种,2019,17(18):6142-6151.[3]王利界,周智彬,常青,等.盐旱交叉胁迫对灰胡杨(Populus pruinosa)幼苗生长和生理生化特性的影响[J].生态学报,2018,38(19):7026-7033.[4]赵新风,朱艳芬,徐海量,等.塔里木河下游21种荒漠植物繁殖体形态特征及对环境的适应[J].西北植物学报,2009,29(2):283-290.[5]史小丽,叶茂,王晓峰,等.NaCl胁迫对塔河下游铃铛刺种子萌发的影响及生理效应[J].种子,2010,29(12):4-8.[6]李涛,尹林克,严成.塔里木河中游天然植被的数量分类与排序研究[J].干旱区地理,2003,26(2):173-179.[7]袁蕾,周华荣,宗召磊,等.乌鲁木齐地区典型灌木群落结构特征及其多样性研究[J].西北植物学报,2014,34(3):595-603.[8]赵义勇,赵虎,李姝漩.铃铛刺花中多糖的含量测定[J].新疆医科大学学报,2017,40(6):116-118.[9]严子柱,李得禄,满多清,等.PEG-6000模拟干旱胁迫对铃铛刺和披针叶野决明种子萌发的影响[J].干旱区研究,2017,34(1):88-94.[10]WANG J,GAO H,ZHAO J,et al.Preparative separation of phenolic compounds from Halimodendeon halodendron by high-speed counter-current chromatography[J].Molecules,2010,15(9):5998-6007.[11]赵义勇,李姝漩,赵虎,等.铃铛刺花提取物对便秘小鼠排便功能的实验研究[J].新疆医科大学学报,2017,40(7):934-936,956.[12]金晓艳,黄红雨,吴冰冰,等.铃铛刺花提取物中总黄酮含量测定[J].亚太传统医药,2017,13(3):41-43.[13]黄红雨,赵虎,王佳,等.铃铛刺花营养成分的测定和评价[J].食品研究与开发,2017,38(7):115-118.[14]张和钰,周华荣,叶琴,等.新疆额尔齐斯河流域典型地区灌木群落多样性[J].生态学杂志,2016,35(5):1188-1196.[15]雷志,阴翠翠,李永亮,等.铃铛刺抗逆转录因子基因HhDREB2的克隆及分析[J].中国农业科技导报,2014,16(4):71-78.[16]赵丽珍,韩路.水分与盐分对荒漠植物种子萌发的影响[J].塔里木大学学报,2012,24(3):96-104.[17]孙霞,高信芬.聚乙二醇(PEG)模拟干旱胁迫对干旱河谷5种木蓝种子萌发的影响[J].应用与环境生物学报,2010,16(3):19-24.[18]HUMARA J M,LOPEZ M,CASSARES A.Temperature and provenance as two factors affecting Eucalyptus nitens need germination[J].Forestry,2000,73(1):87-90.[19]MCLAREN K P,MCDONALD M A.The effects of moisture and shade on seed ger mination and seedling survival in a tropical dry forest in Jamaica[J].Forest Ecology & Management,2003,183:61-75.[20]王娟娟,张文辉,刘新成.NaCl胁迫对3种不同处理四翅滨藜种子萌发的影响[J].西北农业学报,2010,19(1):104-111.[21]吴彦,刘庆,何海,等.光照与温度对云杉和红桦种子萌发的影响[J].应用生态学报,2004,15(12):2229-2232.[22]李志萍,张文辉,崔豫川.NaCl和Na2CO3胁迫对栓皮栎种子萌发及幼苗生长的影响[J].生态学报,2015,35(3):742-751.[23]姜生秀,严子柱,吴昊.PEG-6000模拟干旱胁迫对2种沙冬青种子萌发的影响[J].西北林学院学报,2018,33(5):136-142.[24]孙景宽,张文辉,张洁明.种子萌发期4种植物对干旱胁迫的响应及其抗旱性评价研究[J].西北植物学报,2006,26(9):1811-1818.[25]张肖,王瑞清,李志军.胡杨种子萌发对温光条件和盐旱胁迫的响应特征[J].西北植物学报,2015,35(8):1642-1649.[26]魏振艳,黄海霞,欧阳尔乾,等.水盐胁迫对裸果木种子萌发的影响[J].干旱区研究,2018,35(6):1444-1449.[27]王雨,周睿颖,马立敏,等.5个产地菘蓝种子萌发及幼苗生长对盐胁迫的响应[J].草业学报,2018,27(7):145-154.[28]陈金元,陈学林,满吉琳,等.混合盐碱胁迫对红砂种子萌发的影响[J].西北农林科技大学学报(自然科学版),2016,44(5):113-119.[29]马彦军,祝小娟,何瑞雪.盐爪爪种子萌发期抗盐性研究[J].草地学报,2019,27(5):1237-1242.[30]袁飞敏,权有娟,陈志国.不同钠盐胁迫对藜麦种子萌发的影响[J].干旱区资源与环境,2018,32(11):182-187.[31]刘晓威,杨秀艳,武海雯,等.NaCl胁迫对红砂萌发的影响及萌发期耐盐性评价[J].生物技术通报,2019,35(1):27-34.[32]王志才,牙库甫江·阿西木,王艳,等.水盐胁迫对花花柴种子萌发的影响[J].中国沙漠,2012,32(3):750-755.[33]马彦军,段慧荣,曹致中,等.沙冬青种子萌发期抗逆性研究[J].中国沙漠,2011,31(4):963-967.[34]贺军民,佘小平,张键.番茄种子吸湿-回干处理对盐胁迫伤害的缓解效应[J].园艺学报,2004,27(2):123-126.[35]李威,周玲玉,王恒.干旱胁迫对3个品种红花种子萌发的影响[J].中国农学通报,2013,29(34):207-211.[36]POURESMAEIL H.The physiological effects of water stress by PEG-6000 on germination of mungbean (Vigna radiata) genoytpes[J].International Journal of Agriculture and Crop Sciences,2014,7(13):1344-1345.[37]郭米山,高广磊,丁国栋,等.聚乙二醇模拟干旱胁迫对国槐种子萌发和幼苗生理特征的影响[J].河北农业大学学报,2018,41(6):31-37.[38]何芳兰,赵明,王继和,等.几种荒漠植物种子萌发对干旱胁迫的响应及其抗旱性评价研究[J].干旱区地理,2011,34(1):100-106.[39]BURGASS R W,POWELL A A.Evidence for repair processes in the invigoration of seeds by hydration[J].Annals of Botany,1984,53(5):753-757.[40]吴敏,张文辉,马闯,等.干旱胁迫对栓皮栎种子萌发能力的影响[J].西北农林科技大学学报(自然科学版),2017,45(5):91-100.[41]杨景宁,王彦荣.PEG模拟干旱胁迫对四种荒漠植物种子萌发的影响[J].草业学报,2012,21(6):23-29.[42]史锋厚,朱灿灿,沈永宝,等.PEG-6000渗透处理对油松种子发芽的影响[J].浙江林学院学报,2008,25(3):289-292.[43]罗冬,王明玖,李元恒,等.四种豆科牧草种子萌发和幼苗生长对干旱的响应及抗旱性评价[J].生态环境学报,2015,24(2):224-230.
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备注/Memo
第一作者简介：齐容镰（1996-），女，硕士研究生，研究方向为荒漠化防治。E-mail:1304748118@qq.com.责任作者：李钢铁（1963-），男，博士，教授，博士生导师，现主要从事沙区植物资源保护与利用等研究工作。E-mail:13848817183@163.com.基金项目：内蒙古自治区科技重大专项资助项目。收稿日期：2020-10-09