LI Tian,ZHANG Zhipeng,XU Lurong,et al.Expression Analysis of Related Genes After Inoculation of TYLCV in Tomato[J].Northern Horticulture,2021,(08):1-8.[doi:10.11937/bfyy.20203220]
番茄接种TYLCV后相关基因的表达分析
- Title:
- Expression Analysis of Related Genes After Inoculation of TYLCV in Tomato
- Keywords:
- tomato; TYLCV; different organs; gene expression
- 文献标志码:
- A
- 摘要:
- 以感TYLCV番茄品种“合作909大红番茄”和抗TYLCV番茄品种“华美204”为试材，采用荧光定量PCR技术，研究了TYLCV对感病、抗病番茄植株根、茎、叶片中水杨酸、茉莉酸和乙烯3条防御信号途径中关键因子的表达量的影响，以期了解番茄抗TYLCV的机制。结果表明：接种后30 d，感TYLCV材料“合作909大红番茄”各器官的TYLCV含量均显著高于抗TYLCV番茄材料“华美204”，且根部含量最高，茎部次之，叶片含量最少。水杨酸途径相关基因PR1、NDR1、TGA21在叶片中“合作909大红番茄”的相对表达量显著低于“华美204”，但在茎部和根部中高于“华美204”。NDR1和TGA21在根部的相对表达量最高，茎部次之，叶片最低。茉莉酸途径相关基因LoxD、Pin2在叶片和茎中“合作909大红番茄”的相对表达量低于“华美204”，但在根部高于“华美204”，LoxD、Pin2在根部的相对表达量最高。乙烯途径相关基因ERF3、EIN2在叶片中“合作909大红番茄”的相对表达量低于“华美204”，但在茎部和根部中高于“华美204”，ERF3和EIN2在根部的相对表达量最高，茎部次之，叶片最低。说明水杨酸、茉莉酸、乙烯3条信号传导途径共同参与了番茄抗TYLCV的过程。
- Abstract:
- TYLCV infected tomato variety ‘Cooperative 909 Red Tomato’ and TYLCV resistant tomato variety ‘Huamei 204’ were used as test materials,the effects of TYLCV on the expression levels of key factors in three defense signaling pathways in roots,stems and leaves of susceptible and resistant tomato varieties ‘Cooperative 909 Red Tomato’ and ‘Huamei 204’ were studied by fluorescence quantitative PCR,in order to understand the mechanism of tomato resistance to TYLCV.The results showed that 30 days after inoculation,the content of TYLCV in three organs of the TYLCV infected material ‘Cooperative 909 Red Tomato’ was significantly higher than that of TYLCV resistant material ‘Huamei 204’,and the content of root was the highest,followed by the stem,and the content of leaf was the lowest.The relative expression levels of salicylic acid pathway related genes PR1, NDR1 and TGA21 in leaves of ‘Cooperative 909 Red Tomato’ were significantly lower than ‘Huamei 204’,but higher than ‘Huamei 204’ in stems and roots.The relative expression levels of NDR1 and TGA21 were the highest in root,followed by stem and leaf.The relative expression levels of jasmonic acid pathway related genes LoxD and Pin2 in ‘Cooperative 909 Red Tomato’ were lower than ‘Huamei 204’ in leaves and stems,but higher than ‘Huamei 204’ in roots,with the highest relative expression levels in roots.The relative expression levels of ethylene pathway related genes ERF3 and EIN2 in leaves of ‘Cooperative 909 Red Tomato’ were lower than ‘Huamei 204’,but higher than ‘Huamei 204’ in stems and roots.The relative expressions of ERF3 and EIN2 were the highest in roots,followed by stems and the lowest in leaves.These results indicated the three signal transduction pathways of salicylic acid,jasmonic acid and ethylene were involved in the effect of tomato resistance to TYLCV.
参考文献/References:
[1]刘红霄.抗TYLCV RNAi载体的构建及其转基因番茄的抗病性研究[D].石家庄:河北科技大学,2012.[2]高志勇.番茄的应用价值及其转基因工程研究进展[J].安徽农业科学,2006(9):1864-1865.[3]冯兰香,蔡少华,郑贵彬,等.我国番茄病毒病的主要毒原种类和番茄上烟草花叶病毒株系的鉴定[J].中国农业科学,1987(3):60-66.[4]POLSTON J E,ROSEBROCK T R,SHERWOOD T,et al.Appearance of tomato yellow leaf curl virus in North Carolina[J].Plant Disease,2002,86(1):73.[5]李继纲,梁燕,闫见敏,等.抗TYLCV番茄新品种西农2011的选育[J].中国蔬菜,2012(2):106-109.[6]PIETERSECORN M J,LEON-REYES A,SJOERD V D E,et al.Networking by small-molecule hormones in plant immunity[J].Nature Chemical Biology,2009,5(5):308-316.[7]GRANT M R,JONES J D G.Hormone (dis)harmony moulds plant health and disease[J].Ence,2009,324:750-752.[8]LEON-REYES A,DOES D V D,LANGE E S D,et al.Salicylate-mediated suppression of jasmonate-responsive gene expression in Arabidopsis is targeted downstream of the jasmonate biosynthesis pathway[J].Planta,2010,232(6):1423-1432.[9]李云洲.外源水杨酸诱导RNAi与MAPK3级联信号抗番前黄化曲叶病毒研究[D].杨凌:西北农林科技大学,2017.[10]van LOON L C,van STRIEN E A.The families of pathogenesis-related proteins,their activities,and comparative analysis of PR-1 type proteins[J].Physiological and Molecular Plant Pathology,1999,55(2):85-97.[11]田义,张彩霞,康国栋,等.植物TGA转录因子研究进展[J].中国农业科学,2016,49(4):29-39.[12]FONSECAJ P,MENOSSIM,THIBAUD-NISSENF,et al.Functional analysis of a TGA factor-binding site located in the promoter region controlling salicylic acid-induced NIMIN-1 expression in Arabidopsis[J].Genetics and Molecular Research,2010,9(1):167-175.[13]刘长英,吕蕊花,朱攀攀,等.桑树EIN2基因的分离与表达[C].杭州：第十二届家(柞)蚕遗传育种暨良种繁育学术研讨会,2016.[14]ZHANG H,GONG H R,ZHOU X P.Molecular characterization and pathogenicity of tomato yellow leaf curl virus in China[J].Virus Genes,2009,39(2):249-55.[15]GOVRIN E M,LEVINE A.The hypersensitive response facilitates plant infection by the necrotrophic pathogen Botrytis cinerea[J].Pubmed,2000,10(13):751-757.[16]陆雯,潘璐琪,王雪艳.水杨酸及茉莉酸介导植物抗病性的研究进展[J].贵州农业科学,2013,41(10):40-43.[17]鲁秀梅,张宁,夏美玲,等.不同抗性甜瓜接种蔓枯病菌后内源激素含量变化及其相关基因的表达分析[J].南京农业大学学报,2018,41(2):248-255.[18]王珅,王海燕,刘大群.叶锈菌与‘TcLr19’小麦互作体系中PR1基因的克隆及分析[J].河北农业大学学报,2012,35(2):1-6.[19]田义.苹果抗病相关基因MdTGA21、MdAP2D4与MdAP2D19的克隆与分析[D].泰安:山东农业大学,2013.[20]石延霞,于洋,傅俊范,等.病原菌诱导后黄瓜叶片中脂氧合酶活性与茉莉酸积累的关系[J].植物保护学报,2008,35(6):486-490.[21]娄庆任.花生脂氧合酶及其相关基因表达与黄曲霉产毒抗性的关系[D].北京:中国农业科学院,2013.[22]张春利,袁岐,赵婷婷,等.番茄PIN2基因的生物信息学分析[J].分子植物育种,2017,15(7):2510-2516.[23]BLEECKER A B,KENDE H.Ethylene:A gaseous signal molecule in plants[J].Pubmed,2000,16(1):1-18.[24]CAO Y F,SONG F M,GOODMAN-ROBERT M,et al.Molecular characterization of four rice genes encoding ethylene-responsive transcriptional factors and their expressions in response to biotic and abiotic stress[J].Journal of Plant Physiology,2006,163(11):1167-1178.[25]智军海.桃(Prunus persica(L.)Batsch)乙烯信号转导途径基因CTR1和EIN2的克隆与表达分析[D].杨凌:西北农林科技大学,2010.
相似文献/References:
[1]吴晓燕,张丽荣,马建华,等.一种新型微生物制剂对设施番茄土壤微生物数量的影响[J].北方园艺,2013,37(04):42.
[2]刘配军,李景富,许向阳,等.番茄hp1和dg基因高效检测体系的建立[J].北方园艺,2013,37(04):99.
[3]王晓娥,李建国,赵姝荣,等.咸阳市设施番茄根结线虫病的发生与防治[J].北方园艺,2013,37(04):133.
[4]谷婧玥,李景富,许向阳,等.番茄不同果穗间品质性状差异性研究[J].北方园艺,2014,38(12):26.
GU Jing-yue,LI Jing-fu,XU Xiang-yang,et al.Study on Tomato’s Quality and Traits at Different Tassel[J].Northern Horticulture,2014,38(08):26.
[5]吕 涛,于 杰,赵 勇.日光温室越冬栽培番茄适宜品种筛选试验[J].北方园艺,2014,38(12):40.
LV Tao,YU Jie,ZHAO Yong.Selection of Overwintering Tomato Varieties Suitable for Solar Greenhouse in Heilongjiang[J].Northern Horticulture,2014,38(08):40.
[6]李美芹,裴华丽,乔 宁,等.TYLCV外壳蛋白基因植物表达载体构建及转化番茄的研究[J].北方园艺,2014,38(12):84.
LI Mei-qin,PEI Hua-li,QIAO Ning,et al.Study on the Construction of Plant Expression Vector of TYLCV-CP Gene and Its Transferring Into Tomato[J].Northern Horticulture,2014,38(08):84.
[7]李文枫,李景富,许向阳.不同hp基因类型番茄中番茄红素与主要品质性状的差异及相关分析[J].北方园艺,2014,38(13):97.
LI Wen-feng,LI Jing-fu,XU Xiang-yang.Correlation and Difference Analysis Between Lycopene Content and Main Quality Characters of Tomato with Different hp Gene Types[J].Northern Horticulture,2014,38(08):97.
[8]张 浩,李福云,徐志然,等.不同套作模式对温室连作番茄生长产量及土壤微生物与酶活性的影响[J].北方园艺,2014,38(13):46.
ZHANG Hao,LI Fu-yun,XU Zhi-ran,et al.Effects of Different Relay Intercropping Patterns on Continuous Cropping Tomato Growth，Yield and Soil Microflora and Enzymes Activities in Solar Greenhouse[J].Northern Horticulture,2014,38(08):46.
[9]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2014,38(11):158.
YANG Hui-fang,LIANG Xin-an,CHANG Jie-tian,et al.Effects of Foliar Spraying of Selenium Fertilizer on Selenium Enrichment of Different Vegetables and Yield[J].Northern Horticulture,2014,38(08):158.
[10]祁连弟,管建慧,其日格,等.不同比例牛羊粪便栽培基质对温室番茄苗期生长的影响[J].北方园艺,2013,37(22):47.
QI Lian-di,GUAN Jian-hui,QI Ri-ge,et al.Effects of Different Nursery Substrates with Cow and Sheep Dung on the Seedlings Growth of Tomato in Solar Greenhouse[J].Northern Horticulture,2013,37(08):47.
备注/Memo
第一作者简介：李甜（1996-），女，硕士研究生，研究方向为蔬菜栽培生理。E-mail：2387819693@qq.com.责任作者：孙胜（1977-），男，博士，教授，现主要从事蔬菜栽培生理和蔬菜育种等研究工作。E-mail：sunsheng_2004@126.com.基金项目：山西省科技创新培育团队建设资助项目（201605D131042）；山西省重点研发计划资助项目（201703D211001-04）；上海市科委一带一路国际交流合作资助项目（19390743400）。收稿日期：2020-10-08