[1]郑海霞,王丽,李慧敏,等.核盘菌对观赏向日葵防卫相关基因表达的影响[J].北方园艺,2021,(02):59-64.[doi:10.11937/bfyy.20202680]
　ZHENG Haixia,WANG Li,LI Huimin,et al.Effects of Sclerotinia Sclerotiorum on the Expression of Genes Related to Defensein Ornamental Sunflower[J].Northern Horticulture,2021,(02):59-64.[doi:10.11937/bfyy.20202680]
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核盘菌对观赏向日葵防卫相关基因表达的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年02 页码: 59-64 栏目: 出版日期: 2021-01-30
Title:
Effects of Sclerotinia Sclerotiorum on the Expression of Genes Related to Defensein Ornamental Sunflower
作者:
郑海霞1; 王丽2; 李慧敏1; 李红1; 武海峰1; 张正坤3
（1.沈阳工学院 生命工程学院，辽宁 抚顺 113122；2.吉林省农业科学院 花生研究所,吉林 公主岭 136100；3.吉林省农业科学院 植物保护研究所，吉林 四平 136199）
Author(s):
ZHENG Haixia1; WANG Li2; LI Huimin1; LI Hong1; WU Haifeng1; ZHANG Zhengkun3
(1.Life Engineering College,Shenyang Insititute of Technology,Fushun,Liaoning 113122；2.Peanut Institute,Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136100；3.Plant Protection Institute,Jilin Academy of Agricultural Sciences，Siping,Jilin 136199)
关键词:
观赏向日葵; 核盘菌; 防卫基因
Keywords:
ornamental sunflower; Sclerotinia sclerotiorum; defense gene
DOI:
10.11937/bfyy.20202680
文献标志码:
A
摘要:
以观赏向日葵（Helianthus annuus L.）中一个矮向日葵品种为试材，通过对四叶期观赏向日葵进行核盘菌诱导，采用提取RNA进行反转录、PCR荧光定量等方法研究其防卫相关基因的表达，以期探究核盘菌时观赏向日葵防卫相关基因表达的影响。结果表明：核盘菌侵染对观赏向日葵水杨酸（SA）信号路径中的关键基因[STBX]NPR1、PR1[STBZ]表达量发生上调；ET介导信号传递路径中关键基因[STBX]PDF1.2[STBZ]表达量有所升高；JA/ET途径的关键“节”点基因[STBX]MPK4、EDS1表达量上调变化；NPR1、PR1、EDS1、PDF1.2和MPK4[STBZ]均明显上调表达，由此表明，观赏向日葵受SA和JA信号的调控，核盘菌对观赏向日葵相关防卫基因表达有一定的影响。
Abstract:
A dwarf sunflower variety from ornamental sunflower (Helianthus annuus L.) was used as the test material.By inducing Sclerotinia sclerotiorum to the four-leaf stage ornamental sunflower,RNA was extracted for reverse transcription，PCR fluorescence quantitative methods to study the expression of defense-related genes and to investigate the effect of pantococcus radiodurans on the expression of defense-related genes in ornamental sunflowers.The results showed that Sclerotinia sclerotiorum infection upregulated the expression levels of key genes [STBX]NPR1 and PR1[STBZ] in the SA signaling pathway of ornamental sunflowers;the expression level of key genes [STBX]PDF1.2[STBZ] in the ET-mediated signal transmission pathway increases;JA the expression of the key “node” genes [STBX]MPK4 and EDS1[STBZ] in the ET pathway was up-regulated;[STBX]NPR1,PR1,EDS1,PDF1.2 and MPK4[STBZ] were significantly up-regulated,indicating that ornamental sunflower is regulated by SA and JA signals,and S.sclerotiorum had a certain influence to the expression of defense genes related to ornamental sunflower.

参考文献/References:

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备注/Memo

第一作者简介：郑海霞（1987-），女，硕士，讲师，现主要从事园林植物生理生态及园林生态景观设计等研究工作。E-mail：zhx1988flydream@163.com.责任作者：王丽（1982-），女，硕士，助理研究员，现主要从事观赏向日葵栽培育种等研究工作。E-mail:heboo123@163.com.收稿日期：2020-06-27

更新日期/Last Update: 2021-05-19