ZHANG Jiaxin,LI Jingyan,ZHU Shaowen,et al.Genetic Diversity Analysis of Pea Germplasm Resources[J].Northern Horticulture,2021,(02):1-9.[doi:10.11937/bfyy.20202088]
引进豌豆种质资源遗传多样性分析
- Title:
- Genetic Diversity Analysis of Pea Germplasm Resources
- Keywords:
- peas; phenotypic traits; SSR; seed resources; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以引进的34份豌豆种质资源为试材，采用19个表型性状对其变异性、多样性和主成分分析进行了研究，结合SSR分子标记对其开展遗传多样性分析，以期加速豌豆种子资源研究进程。结果表明：19个性状在不同材料之间存在显著差异，平均变异系数为45.05%，平均遗传多样性指数为0.95；前7个成分占表达信息量的74.807%，其中第1主成分占表达信息量的18.350%，且第1主成分在脐色、粒色、叶面积、茎粗和花色等性状呈现较高的载荷量；表型性状分析将34份材料分成8类；分子标记研究结果显示，118对SSR引物共筛选出25对具有稳定多态性条带，共检测115个差异位点，每对引物平均4.6个，有效等位变异数为1.694 4，有效等位变异所占比重为40.17%，Shannon信息指数为0.594 1。运用NTSYS软件进行聚类分析，34份材料遗传相似系数(GS)为0.461~0.860，平均0.661，SSR标记分析引进的34份材料也被分为8类，2种分类方法呈现显著正相关（R=0.901 3，P=0.101 1>0.050）。该研究利用SSR分子标记从分子水平上揭示豌豆种质资源遗传背景和亲缘关系，可为今后豌豆资源的利用提供重要参考依据。
- Abstract:
- Taking 34 pea germplasm resources as test materials,19 phenotypic traits were used to study the variability,diversity and principal component analysis,and the genetic diversity analysis was carried out in combination with SSR molecular markers in order to accelerate the research process of pea seed resources.The results showed that there were significant differences among the 19 traits among different materials,the average coefficient of variation was 45.05%,and the average genetic diversity index was 0.95.The first 7 components accounted for 74.807% of the expression information,among which the first principal component accounted for 18.350% of the expression information,and the first principal component presented a higher load in umbilical color,grain color,leaf area,stem diameter and color.Phenotypic trait analysis divided 34 materials into eight categories.Molecular marker study results showed that a total of 25 pairs of SSR primers with stable polymorphism were screened out from 118 pairs,115 difference sites were detected,with an average of 4.6 for each pair of primers,the effective allelic variation was 1.694 4,the proportion of effective allelic variation was 40.17%,and Shannon’s diversity information index was 0.594 1.By using NTSYS software progressive clustering analysis,the genetic similarity coefficient (GS) of 34 materials was 0.461-0.860,with an average of 0.661.The 34 materials introduced by SSR labeling analysis were also divided into eight categories.The correlation between the results of the two classification methods was (R=0.901 3,P=0.101 1>0.050) with a significant positive correlation.This study used SSR molecular markers to reveal the genetic background and relationship of pea germplasm resources at the molecular level,providing an important basis for the utilization of pea resources in the future.
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备注/Memo
第一作者简介：张佳欣(1995-)，女，硕士研究生，研究方向为蔬菜遗传育种。E-mail：859511129@qq.com.责任作者：纪鹏（1978-），男，博士，副教授，现主要从事园艺学等研究工作。E-mail：1526291014@qq.com.基金项目：国家自然基金资助项目（31772330）；黑龙江省自然基金资助项目（C2017054）。收稿日期：2020-05-21