LI Yanyan,WANG Qianqian,SU Yuanyuan,et al.Extraction of Total Flavonoid in Taxus media by Response Surface Methodology and Its Antioxidant Activity[J].Northern Horticulture,2021,(05):94-102.[doi:10.11937/bfyy.20201562]
响应面法优化提取曼地亚红豆杉总黄酮及抗氧化活性
- Title:
- Extraction of Total Flavonoid in Taxus media by Response Surface Methodology and Its Antioxidant Activity
- 文献标志码:
- A
- 摘要:
- 以曼地亚红豆杉枝叶为试材，采用热回流法提取总黄酮，在单因素试验的基础上，采用 Box-Benhken设计对曼地亚红豆杉枝叶总黄酮的热回流提取工艺进行优化，对其抗氧化活性进行研究，以期为曼地亚红豆杉的进一步开发利用提供参考依据。结果表明：最佳提取条件为液料比32∶1 mL?g-1，乙醇浓度63%，提取温度71 ℃，提取时间86 min，提取次数2次，该条件下曼地亚红豆杉枝叶中总黄酮的提取量为128.1 mg?g-1，该提取工艺稳定可行。曼地亚红豆杉枝叶总黄酮在设定最大质量浓度时，DPPH自由基、ABTS+自由基和亚硝酸盐的清除率分别为91.04%、99.17%和65.50%，表明曼地亚红豆杉枝叶总黄酮具有较强的体外抗氧化能力。
- Abstract:
- Taking the branches and leaves of Taxus media as experiment materials,on the basis of single factor experiment,the response surface method was used to optimize the thermal reflux ectraction process of total flavonoid from Taxus media,and the scavenging rate of DPPH?,ABTS+? and nitrite radicals were used to study the antioxidant activity of total flavonoid,in order to provide a reference for the further development and utilization of T.media.The results showed that the optimal extraction process was as following,the liquid-material ratio was 32∶1 mL?g-1,ehanol concentration was 63%,extraction temperature was 71 ℃ and extraction time was 86 minutes,the number of extraction was 2 times.Under the above conditions,the extraction yield of total flavonoid from T.media was up to 128.10 mg?g-1.The extraction rate of total flavonoid was higher in extraction process,therefore the extraction technology of total flavonoid from T.media was stable and applicable.At the maximum total flavonoid extract concentration,the scavenging rates of DPPH?,ABTS+? and nitrite were 91.04%,99.17% and 65.50%,respectively.The result indicated that the antioxidant activity from T.media was strong in vitro.
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备注/Memo
第一作者简介：李艳艳（1984-），女，博士，讲师，现主要从事天然产物提取等研究工作。E-mail:liyanyan041@163.com.责任作者：王俊青（1981-），男，硕士，工程师，现主要从事天然产物提取等研究工作。E-mail:173019503@qq.com.基金项目：国家自然科学基金资助项目（31700597）；林木遗传育种国家重点实验室开放课题资助项目（TGB2019003）；平顶山学院青年科研基金资助项目（PXYQNJJ2016018）；平顶山学院高层次人才科研启动基金资助项目（PXY-BSQD2016005）；平顶山学院2017年度国家级科研项目培育基金资助项目（PXY-PYJJ2017005）。收稿日期：2020-04-17