[1]李辉,庞建周,魏淑珍,等.蚯蚓粪中高效解磷菌的筛选及解磷特性的研究[J].北方园艺,2020,44(24):76-81.[doi:10.11937/bfyy.20200631]
　LI Hui,PANG Jianzhou,WEI Shuzhen,et al.Screening and Identification of High Efficient Phosphate-solubilizing Bacteria From Vermicompost[J].Northern Horticulture,2020,44(24):76-81.[doi:10.11937/bfyy.20200631]
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蚯蚓粪中高效解磷菌的筛选及解磷特性的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年24 页码: 76-81 栏目: 出版日期: 2020-12-30
Title:
Screening and Identification of High Efficient Phosphate-solubilizing Bacteria From Vermicompost
作者:
李辉1; 庞建周2; 魏淑珍1; 王茜3; 崔恩溪3; 张珠琛3
（1.衡水学院 湿地保护与研究中心，河北 衡水 053000；2.河北省农林科学院 旱作农业研究所，河北 衡水 053000；3.衡水学院 生命科学学院，河北 衡水 053000）
Author(s):
LI Hui1; PANG Jianzhou2; WEI Shuzhen1; WANG Qian3; CUI Enxi3; ZHANG Zhuchen3
(1.Centre for Wetland Conservation and Research,University of Hengshui,Hengshui,Hebei 053000;2.Institute of Dry Farming,Hebei Academy of Agriculture and Forestry Sciences,Hengshui,Hebei 053000;3.Department of Biology,University of Hengshui,Hengshui,Hebei 053000)
关键词:
解磷菌; 菌种鉴定; 可溶性磷; 解磷能力
Keywords:
phosphate-solubilizing bacteria; identification; soluble phosphorus; phosphate solubilizing power
DOI:
10.11937/bfyy.20200631
文献标志码:
A
摘要:
以新鲜的蚯蚓粪为试材，采用选择性培养基初筛和钼锑抗比色法复筛筛选解磷菌，共分离筛选得到7株解磷菌LH01、LH02、LH03、LH04、LH05、LH06、LH07，其中LH05菌的解磷能力最强，解磷圈的内外直径比D/d达2.26。根据LH05菌体形态观察、生理生化特性鉴定及16S rDNA同源性分析等结果，初步鉴定该菌为短小芽孢杆菌（Bacillus pumilus）。结果表明：LH05菌解磷的最佳条件分别为接种量9％、培养温度35 ℃、初始pH 6.5，可溶性磷含量最高可达96.69 mg?L-1。该研究结果为进一步研制高效解磷菌剂提供稳定高效的菌种和前期研究基础。
Abstract:
The earthworm manure was used as the material in this study.Seven strains of phosphate bacteria LH01,LH02,LH03,LH04,LH05,LH06,and LH07 were isolated and screened by selective medium primary screening and molybdenum-antimony anticolorimetry.Among them, the LH05 bacteria had the strongest ability to dissolve phosphorus, and the ratio of the inner and outer diameters of the phosphate ring was 2.26.According to the results of morphological characteristics,physiological and biochemical identification and 16S rDNA homology analysis,the bacterium was preliminarily identified as Bacillus pumilus.The results showed that orthogonal experiment,optimum conditions of phosphorus removal of LH05 were 9% inoculum amount,35 ℃ culture temperature,initial pH 6.5 and 96.69 mg?L-1 dissoluble phosphorus content.Which provided stable and high-efficient bacteria and theoretical basis for further development of high-efficiency phosphorus removal bacteria.

参考文献/References:

[1]唐岷宸,李文静,宋天顺,等.一株高效解磷菌的筛选及其解磷效果验证[J].生物技术通报,2020,36(6):1-8.[2]王艳霞,解志红,张蕾,等.田菁根际促生菌的筛选及其促生耐盐效果[J].微生物学报,2020,60(5):1023-1035.[3]SAURABH J S,SATINDER K B,YANN L B,et al.Potential application of biohydrogen production liquid waste as phosphate solubilizing agent:A study using soybean plants[J].Appl Biochem Biotechnol,2016,178:865-875.[4]严漪云.污泥蚓粪对滩涂土壤重要理化性质的影响及培肥效应[D].扬州:扬州大学,2019.[5]严漪云,左文刚,徐凯达,等.污泥蚓粪对滩涂盐碱地土壤的培肥效应[J].扬州大学学报(农业与生命科学版),2019(3):111-116.[6]张孟豪,吴家龙,张池,等.赤子爱胜蚓对赤红壤铝形态的影响[J].华南农业大学学报,2020,41(2):48-54.[7]韩雪娇,曾庆伟,赵玉萍.杨树根际解无机磷细菌Mp1-Ha4的鉴定及其解磷机理[J].生物技术通报,2020,36(3):141-147[8]向万胜,黄敏,李学垣,等.土壤磷素的化学组分及其植物有效性[J].植物营养与肥料学报,2004,10(6):663-670.[9]周德庆.微生物学实验手册[M].上海:上海科学技术出版社,1986.[10]东秀珠.常见细菌系统鉴定手册[M].北京:北京科学出版社,2001.[11]梁国栋.最新分子生物学实验技术[M].北京:科学出版社,2001.[12]韩蕾,杨乐,唐金铭,等.解磷菌发酵及溶磷条件的研究[J].生物技术通报,2019(1):98-104.[13]李海云,牛世全,孔维宝,等.猪粪堆肥中一株溶磷菌的筛选、鉴定及溶磷能力初步测定[J].环境科学学报,2015,35(5):1464-1470.[14]苏辉兰,莫雪雪,余炳锋,等.贡柑果园高效解磷菌的筛选及其解磷条件优化[J].北方园艺,2019(5):101-107.[15]吴海波,王樱潼,叶维雁,等.4株番木瓜根际解磷细菌发酵条件优化研究[J].西南林业大学学报(自然科学版),2018,38(2):117-125.[16]LIU F P,LIU H Q,ZHOU H L,et al.Isolation and charac-terization of phosphate-solubilizing bacteria from betel nut (Arecacatechu) and their effects on plant growth and phosphorus mobilization in tropical soils[J].Biol Fertil Soils,2014,50:927.[17]杜雷,王素萍,陈钢,等.一株高效解磷细菌的筛选、鉴定及其溶磷能力的研究[J].中国土壤与肥料,2017(3):136-141.[18]李学平,谢文军,范延辉,等.盐碱地塔宾曲霉菌的解磷能力及其对小麦生长的影响[J].水土保持通报,2017,37(1):93-96.[19]柯春亮,陈宇丰,周登博,等.香蕉根际土壤解磷细菌的筛选、鉴定及解磷能力[J].微生物学通报,2015,42(6):1032-1042.[20]李海峰,张月阳,曹健,等.耐寡营养高效解磷菌株的分离鉴定及解磷特性[J].河南农业科学,2017,46(8):67-71.[21]王样庭,陈玲,王陶,等.牛蒡根际土壤解磷菌的筛选、鉴定及生长特性[J].河南农业科学,2018,47(10):57-63.

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备注/Memo

第一作者简介：李辉（1984-），女，硕士，实验师，现主要从事环境微生物生态学等研究工作。E-mail:lflihui@163.com.责任作者：庞建周（1983-），男，博士，副研究员，现主要从事分子生物学等研究工作。E-mail:236209526@qq.com.基金项目：2018年衡水市科技计划资助项目（2018014029Z）;2018年河北省省级科技计划资助项目（18222915）。收稿日期：2020-02-24

更新日期/Last Update: 2021-03-29