[1]张蕊,田叶韩,李超,等.球毛壳菌ND35对黄瓜种子萌发和胚根生长的影响[J].北方园艺,2020,44(23):1-9.[doi:10.11937/bfyy.20200554]
　ZHANG Rui,TIAN Yehan,LI Chao,et al.Effects of Chaetomium globosum ND35 on Germination and Radicle Growth of Cucumber Seeds[J].Northern Horticulture,2020,44(23):1-9.[doi:10.11937/bfyy.20200554]
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球毛壳菌ND35对黄瓜种子萌发和胚根生长的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年23 页码: 1-9 栏目: 出版日期: 2020-12-15
Title:
Effects of Chaetomium globosum ND35 on Germination and Radicle Growth of Cucumber Seeds
作者:
张蕊1; 田叶韩1; 李超2; 何邦令1; 高克祥1
（1.山东农业大学 植物保护学院，山东省蔬菜病虫生物学重点实验室，山东 泰安 271018;2.泰安市徂徕山林场，山东 泰安 271000）
Author(s):
ZHANG Rui1; TIAN Yehan1; LI Chao2; HE Bangling1; GAO Kexiang1
(1.College of Plant Protection,Shandong Agricultural University/Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests,Tai′an,Shandong 271018;2.Forestry Farm of Culai Mountain of Tai′an City,Tai′an,Shandong 271000)
关键词:
球毛壳菌ND35; 黄瓜; 种子萌发; 内源激素; 酶活性
Keywords:
Chaetomium globosum ND35; cucumber; seed germination; endogenous hormone; enzyme activity
DOI:
10.11937/bfyy.20200554
文献标志码:
A
摘要:
以黄瓜“津春4号”为试材，采用双层滤纸培养方式，不同浓度（1×104、1×105、1×106、1×107、1×108 cfu·mL-1）的球毛壳菌（Chaetomium globosum）ND35孢子悬浮液处理黄瓜种子，研究了不同浓度球毛壳菌ND35孢子悬浮液对黄瓜种子萌发及胚根生长的影响，并进一步研究最适浓度（1×106 cfu·mL-1）的球毛壳菌ND35孢子悬浮液对黄瓜种子萌发期间的保护酶活性、活性氧含量和内源激素含量的影响，以期为球毛壳菌ND35在黄瓜育苗中的应用提供参考依据。结果表明：1×104~1×106 cfu·mL-1球毛壳菌ND35孢子悬浮液处理可以促进黄瓜种子萌发和胚根生长，随着浓度的升高其促进作用均有增加趋势，而1×107~1×108 cfu·mL-1球毛壳菌ND35孢子悬浮液对黄瓜种子萌发和胚根生长有抑制作用。且以1×106 cfu·mL-1球毛壳菌ND35处理对黄瓜种子的发芽势、发芽指数、活力指数、胚根长和鲜质量的效果最佳，分别提高了56.94%、19.92%、100.12%、66.89%和449.07%。催芽48 h后，ND35处理的黄瓜种子发芽率达100%，根长显著高于对照117.18%。1×106 cfu·mL-1球毛壳菌ND35处理可以提高黄瓜萌发期间过氧化物酶（POD）和苯丙氨酸解氨酶（PAL）活性，并且活性氧（ROS）、过氧化氢（H2O2）及内源激素赤霉素（GA）、水杨酸（SA）、生长素（IAA）、茉莉素（JA）的含量均有不同程度的提高。
Abstract:
‘Jinchun No.4’ cucumber seed was used as experimental materials,the double filter paper culture method was used,the concentrations（1×104 cfu·mL-1,1×105 cfu·mL-1,1×106 cfu·mL-1,1×107 cfu·mL-1,1×108 cfu·mL-1 of Chaetomium globosum ND35 spore suspension were used to treat cucumber seeds.Effects of different concentrations of C.globosum ND35 spore suspension treatment on seeds germination of cucumber and radicle growth were investigated,and the optimum concentration (1×106 cfu·mL-1) of C.globosum ND35 spore suspension on the protective enzyme activity,reactive oxygen content and endogenous hormones content during cucumber seeds germination were furtherly studied,in order to provide a reference for the application of C.globosum ND35 in cucumber seedlings.The results showed that 1×104-1×106 cfu·mL-1 of C.globosum ND35 spore suspension could promote the germination and radicle growth of cucumber seeds,and the promotion effect increases with the increasing of concentration,however 1×107-1×108 cfu·mL-1 of C.globosum ND35 spore suspension could inhibited germination and radicle growth of cucumber seeds.And 1×106 cfu·mL-1 of C.globosum ND35 spore suspension treatment on the germination potential,germination index,vitality index,radicle length and fresh weight of cucumber seeds was the best,which increased by 56.94%,19.92%,100.12%,66.89% and 449.07%,compared with CK.Cultivation of cucumber seeds treated with C.globosum ND35 spore suspension (1×106 cfu·mL-1) for 48 hours,the germination rate reached 100%,and the root length was significantly higher 117.18% than CK.1×106 cfu·mL-1 of C.globosum ND35 spore suspension could increased the activities of peroxidase (POD),phenylalanine ammonia-lyase (PAL) and plant reactive oxygen species (ROS),hydrogen oxide (H2O2),and endogenous hormones gibberellin (GA),salicylic acid (SA),indole-3-acetic acid (IAA),jasmonic acid (JA) were all increased varying degrees.

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备注/Memo

第一作者简介：张蕊（1990-），女，硕士研究生，研究方向为植物病害生物防治。E-mail:ruizhangzrr@163.com.责任作者：高克祥（1963-），男，博士，教授，博士生导师，现主要从事植物病害生物防治等研究工作。E-mail:kxgao63@163.com.基金项目：山东省重点研发计划资助项目（2018GNC111005）；山东省重大科技创新工程资助项目（2017CXGC0207，2019JZZY010714）；公益性行业（农业）科研专项经费资助项目（201503110-12）。收稿日期：2020-02-17

更新日期/Last Update: 2021-02-18