YAN Haixia,GUAN Shikai,TAO Dayan,et al.Study on Leaf Cutting Propagation of Two Kinds of Gesneriaceae Based on Orthogonal Test[J].Northern Horticulture,2021,(02):65-72.[doi:10.11937/bfyy.20200463]
基于正交实验的两种苦苣苔叶片扦插繁殖研究
- Title:
- Study on Leaf Cutting Propagation of Two Kinds of Gesneriaceae Based on Orthogonal Test
- Keywords:
- gesneriaceae; leaf cutting; substrate; new plant
- 文献标志码:
- A
- 摘要:
- 以苦苣苔品种Primulina dryas ‘Hisako’和Primulina ‘Blue Mood’为试材,采用正交实验设计,分析不同因素、不同基质组分对扦插成活率、子株数、子株叶数的影响,并筛选出最优组合。探讨影响报春苣苔属扦插成活的因素,为报春苣苔属的扦插繁殖技术提供参考依据。结果表明:3种不同因素对Primulina dryas ‘Hisako’成活率的影响大小为浓度>基质>叶片部位,对子株数的影响大小为叶片部位>浓度>基质,对子株叶数的影响大小为基质>叶片部位>浓度,最优组合为IBA+NAA等量混合浓度100 mg?L-1+泥炭:珍珠岩:蛭石的体积比2∶1∶1+叶片下部。4种基质组分对P.‘Blue Mood’成活率的影响大小为泥炭>稻壳炭>蛭石>珍珠岩,对子株数的影响大小为珍珠岩>泥炭>蛭石>稻壳炭,对子株叶数的影响大小为泥炭>稻壳炭>珍珠岩>蛭石,扦插基质最优组合为泥炭、珍珠岩、蛭石、稻壳炭的体积比6∶2∶2∶1。
- Abstract:
- In order to provide reference for the cutting propagation technology of Primulia,the factors influencing the cutting survival of Primulia were discussed.With Primulina Dryas ‘Hisako’ and Primulina ‘Blue Mood’ as materials,orthogonal experiment design was used to study.Three factors and three levels test were carried out on P.Dryas ‘Hisako’ and four factors and three levels test were carried out on P.‘Blue Mood’.The effects of different factors and matrix components on the survival rate,number of new plants and leaves of new plants were analyzed,and the best combination was selected.The results showed that the influence of three different factors on the survival rate of P.Dryas ‘Hisako’ was concentration>substrate>leaf position,the influence on the number of new plant was leaf position>concentration>substrate,the influence on the number of new plant′s leaves was substrate>leaf position>concentration,the optimal combination was IBA+NAA equal mixed concentration of 100 mg?L-1+peat∶perlite∶vermiculite volume ratio of 2∶1∶1+lower part of leaf.The effect of the four substrate components on the survival rate of P.‘Blue Mood’ was peat>rice husk charcoal>vermiculite>perlite,the effect on the number of new plant was perlite>peat>vermiculite>rice husk charcoal,the effect on the number of leaves were peat>rice husk charcoal>perlite>vermiculite,the optimal combination of cutting substrate was peat,perlite,vermiculite,rice husk charcoal volume ratio of 6∶2∶2∶1.
参考文献/References:
[1]闫海霞,黄昌艳,关世凯,等.5种报春苣苔属植物的叶插繁殖研究[J].热带作物学报,2018,39(1):20-26.[2]赵伟.3种国产唇柱苣苔属野生花卉的组培、扦插和幼苗半致死温度研究[D].上海:上海师范大学,2015.[3]周太久,黄仕训,邓涛,等.6种唇柱苣苔属植物叶插繁殖试验[J].南方农业学报,2012,43(3):356-359.[4]李谦盛,瞿家莺,沈丹峰,等.卡柱苣苔叶片扦插繁殖技术初探[J].浙江农业学报,2014,26(3):675-679.[5]吕德任,任军方,符瑞侃,等.非洲紫罗兰离体叶片不定芽的诱导及植株再生[J].绿色科技,2018(23):77-79.[6]杨珺,符瑞侃,戚华沙,等.绣毛马铃苣苔叶片的离体培养[J].分子植物育种,2018,16(8):2606-2611.[7]林茂,李进华,唐庆,等.大根唇柱苣苔的组培快繁技术[J].热带作物学报,2017,38(3):508-512.[8]林茂,李进华,唐庆,等.3苞唇柱苣苔离体培养体系的建立[J].西南农业学报,2017,30(1):199-204.[9]闫海霞,黄昌艳,张自斌,等.贵港报春苣苔的叶片组培与植株再生[J].热带作物学报,2019,40(1):98-106.[10]闫海霞,关世凯,邓杰玲,等.黄花牛耳朵离体叶片不定芽的诱导及植株再生[J].分子植物育种,2018,16(1):211-216.[11]闫海霞,邓杰玲,黄昌艳,等.褐纹报春苣苔组织培养与快速繁殖[J].广西植物,2017,37(10):1270-1278.[12]闫海霞,关世凯,黄昌艳,等.羽裂报春苣苔的组织培养研究[J].热带作物学报,2017,38(9):1671-1676.[13]闫海霞,邓杰玲,何荆洲,等.永福报春苣苔离体快繁体系的建立[J].植物生理学报,2017,53(11):2037-2043.[14]闫海霞,关世凯,周锦业,等.基于正交实验的大根报春苣苔扦插影响因素优化[J].南方农业学报,2019,50(11):2519-2524.[15]潘春柳,彭玉德,吕惠珍.广西特有药用植物弄岗唇柱苣苔叶插繁殖研究[J].北方园艺,2011(1):205-207.[16]温放,张启翔.5种唇柱苣苔属植物叶插繁殖方式研究[J].北方园艺,2007(12):103-105.[17]温放.广西苦苣苔科观赏植物资源调查与引种研究[D].北京:北京林业大学,2008.[18]唐文秀,黄仕训,隗红燕,等.石灰岩植物桂林唇柱苣苔叶插繁殖研究[J].西南农业学报,2009,22(5):1395-1399.[19]艾春晓,罗乐,张启翔,等.4种报春苣苔属植物叶插繁殖技术研究[J].广东农业科学,2013,40(6):43-46.
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备注/Memo
第一作者简介:闫海霞(1981-),女,硕士,副研究员,现主要从事花卉新品种选育与示范推广等工作。E-mail:819307232@qq.com.责任作者:罗述名(1965-),男,博士,研究员,现主要从事观赏植物新品种选育及推广应用等工作。E-mail:shumingluo17@163.com.基金项目:广西创新驱动发展专项资金资助项目(桂科AA17204046-1,桂科AA17204045-6);广西农业科学院基本科研业务专项资助项目(桂农科2020YM33,桂农科2019M08,桂农科2018YT33)。收稿日期:2020-02-11