LI Minghu,LUO Yuying,JIA Weijia,et al.Effects of Different Nitrogen Forms on the Growth and Rhizosphere Bacterial Community Structure of Chimonanthus campanulatus[J].Northern Horticulture,2020,44(19):86-96.[doi:10.11937/bfyy.20194186]
不同氮肥对西南蜡梅幼苗生长及根际土壤细菌群落的影响
- Title:
- Effects of Different Nitrogen Forms on the Growth and Rhizosphere Bacterial Community Structure of Chimonanthus campanulatus
- 文献标志码:
- A
- 摘要:
- 以盆栽西南蜡梅为试材，对西南蜡梅种植土壤施用不同氮肥（CK、N1（NH4NO3）、N2（KNO3）、N3（C2H5NO2）、N4（NH4Cl）），采用荧光定量PCR和变性梯度凝胶电泳技术，研究不同氮肥对西南蜡梅根际土壤细菌群落的影响，分析其理化性质、土壤细菌群落及植物生长的关系，以期为西南蜡梅推广栽培、科学施肥和保护土壤生态系统提供参考依据。结果表明：氮肥处理后植株株高和干质量均高于CK，且均为N1最高；qPCR结果显示，与CK相比，N1的根际细菌拷贝数最高；DGGE图谱分析显示，氮肥处理后的土壤细菌多样性指数均大于对照；相关性分析表明，细菌拷贝数及多样性指数均与西南蜡梅幼苗植株生长呈极显著相关（P<0.01）。综合分析，不同氮肥处理对植株生长发育和土壤微生物数量及群落结构影响明显，施NH4NO3对盆栽西南蜡梅幼苗植株生长发育及根际土壤细菌拷贝数、多样性指数促进作用最好。
- Abstract:
- Chimonanthus campanulatus pot seedling was used as material，different forms (CK，N1(NH4NO3)，N2(KNO3)，N3(C2H5NO2) and N4(NH4Cl)) of nitrogen fertilizer were applied to planting soil，the influence of different nitrogen fertilizers on the bacterial community in the rhizosphere soil of Chimonanthus campanulatus were studied by real-time PCR and DGGE.In order to provide reference for the promotion and cultivation of Chimonanthus campanulatus，scientific fertilization and protection of soil ecosystems，the relationship between physical and chemical properties，soil bacterial community and plant growth were analyzed.The results showed that Chimonanthus campanulatus seedling height and dry weight of the nitrogen treatments were both higher than CK，and the highest was N1.The results of qPCR showed that the rhizosphere bacteria abundance of N1 was the highest compared with CK.DGGE analysis showed that the bacterial diversity index of nitrogen treatments was higher than CK.Correlation analysis showed that the bacteria abundance and diversity index were significantly correlated with the growth of Chimonanthus campanulatus (P<0.01).After comprehensive analysis，the nitrogen fertilizer treatment had significant effects on plant growth and development，soil microbial quantity and community structure.NH4NO3 was the best for the growth and development of Chimonanthus campanulatus and the amount and diversity index of rhizosphere soil bacteria.
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备注/Memo
第一作者简介：李明虎（1995-），男，硕士研究生，研究方向为园林植物应用。E-mail:449209000@qq.com.责任作者：王澍（1980-），男，博士，副教授，现主要从事园林植物等研究工作。E-mail:613636661@qq.com.基金项目：国家自然科学基金资助项目（31860229）。收稿日期：2019-11-06