JIANG Huamei,WANG Xiangqian,LIANG Honghua,et al.Optimization of Ultrasonic-microwave Synergistic Extraction Process for Rhynchophylline From Uncaria laevigata Wal.ex G.Don in Uncaria of Jianhe[J].Northern Horticulture,2020,44(16):98-104.[doi:10.11937/bfyy.20193943]
剑河双钩藤中钩藤碱的超声-微波协同萃取条件优化
- Title:
- Optimization of Ultrasonic-microwave Synergistic Extraction Process for Rhynchophylline From Uncaria laevigata Wal.ex G.Don in Uncaria of Jianhe
- Keywords:
- Jianhe; Uncaria laevigata Wal.ex G.Don ; rhynchophylline; ultrasonic-microwave synergistic extraction; response surface methodology
- 文献标志码:
- A
- 摘要:
- 以剑河双钩藤为试材，比较了微波辅助萃取、超声波辅助萃取、超声-微波协同萃取3种方法对钩藤碱得率的影响，在单因素试验基础上，采用Box-Behnken响〖JP+2〗应面法优化超声-微波协同萃取剑河双钩藤钩藤碱提取工艺，以期得到超声-微波协同萃取钩藤碱的最佳工艺参数。结果表明：超声-微波协同萃取法的钩藤碱得率远高于超声波、微波萃取法。最佳超声-微波协同萃取工艺参数为萃取时间15 min、乙醇体积分数46%、料液比1∶208 g?mL-1，超声波功率150 W，微波功率150 W。在该条件下，剑河双钩藤钩藤碱的得率为52.928 1 mg?g-1，与模型预测值52.957 9 mg?g-1相近。优选的超声-微波协同萃取工艺提高了剑河双钩藤中钩藤碱得率。
- Abstract:
- Rhynchophylline from Uncaria laevigata Wal.ex G.Don as test material,the effects of microwave-assisted extraction,ultrasound-assisted extraction and ultrasound-microwave synergistic extraction on the yield of rhynchophylline were compared,on the basis of single factor experiment,the optimum ultrasound-microwave synergistic extraction process of the rhynchophylline from Uncaria laevigata Wal.ex G.Don in Jianhe was analyzed by using Box-Behnken design (BBD) with response surface methodology,in order to obtain the optimal ultrasonic-microwave synergistic extraction parameters for rhynchophylline.The results showed that the yield of rhynchophylline by ultrasonic-microwave synergistic extraction was much higher than that of ultrasonic-assisted and microwave-assisted extraction.The optimal ultrasonic-microwave synergistic extraction parameters for rhynchophylline from Uncaria laevigata Wal.ex G.Don in Jianhe were determined as follows 15 minutes of extraction time,46% of ethanol volume fraction,1∶208 g?mL-1 of solid-liquid ratio,150 W of ultrasonic powder,150 W of microwave powder.Under these conditions,the yield of rhynchophylline from Uncaria laevigata Wal.ex G.Don in Jianhe was 52.928 1 mg?g-1,which was cloded to the model predicted value of 52.957 9 mg?g-1.The optimal ultrasound-microwave synergistic extraction process improved the yield of rhynchophylline from Uncaria laevigata Wal.ex G.Don in Jianhe.
参考文献/References:
[1]中国药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2010:240.[2]中国科学院药用植物资源开发研究所.中药志(第五册)[M].北京:人民卫生出版社,1994.[3]罗鸣.剑河钩藤优良品系筛选研究[D].贵阳:贵州师范大学,2015.[4]彭先彦.剑河钩藤的历史渊源和标准化种植[C].佳木斯:全国农业标准化研讨会,2013.[5]蒲锹邈.剑河钩藤种植与生态养鸡种养结合的成效[J].畜禽与水产工程,2017(1):71.[6]杨鑫,谭霖,王廷龙,等.黔产钩藤中钩藤碱的测定及限量研究[J].贵州科学,2014,32(4):67-70.[7]徐东升,杨春,叶世芸,等.贵州中药材钩藤主要有效成分总生物碱含量的测定[J].贵阳中医学院学报,2014,36(4):30-32.[8]蒋华梅,王向前,张娇娇.响应面法优化微波提取剑河双钩藤中的钩藤碱[J].食品工业,2019,40(5):159-163.[9]张清海,刘红,罗爱芹,等.贵州剑河钩藤不同药用部位十五种元素特征分析[J].北方园艺,2016(16):156-159.[10]李金玲,赵致,龙安林,等.贵州不同来源地钩藤药材中8种矿质元素含量分析[J].中国野生植物资源,2015,34(1):22-25.[11]林红,张清海,林绍霞,等.贵州钩藤不同产地钧、茎微量元素比较分析[J].贵州科学,2014,32(1):31-34.[12]唐路梅,夏新华.中药钩藤最优醇提工艺研究[J].中医药导报,2014,20(4):71-74.[13]吴卫,唐振祥,李素珍.酶解法辅助提取钩藤中钩藤碱的工艺优选[J].中国实验方剂学杂志,2015,21(10):23-25.[14]刘婷婷,郁颖佳,段更利,等.离子液体-微波辅助提取钩藤中生物碱的工艺研究[J].中国新药与临床杂志,2013,32(6):482-486.[15]钟帼歆,富志军.钩藤中钩藤总生物碱的含量测定[J].中医药临床杂志，2012,24(8):781-782.[16]王盟,刘卫,邱庆浩,等.正交实验法优选钩藤总碱提取工艺[J].医药导报,2009,28(9):1200-1201.[17]杜玉兰,黎庆涛,王远辉.响应曲面法优化鼠尾藻中脂质的提取工艺[J].天然产物研究与开发,2008,20(6):1091-1094.
备注/Memo
第一作者简介：蒋华梅(1976-),女,硕士，教授,现主要从事天然产物化学等研究工作。E-mail:857346695@qq.com.基金项目：贵阳市财政支持贵阳学院学科建设与研究生教育资助项目（HC-2020）；贵州省高校优秀科技创新人才支持计划资助项目（黔教合KY字[2015]500）；贵州省大学生创新创业训练计划资助项目（20195201398,20195201351）。收稿日期：2019-10-20