LYU Shuping,MI Lanfang,WANG Xuexiong,et al.Resistance of Some Navel Orange Varieties to Canker Disease in Gannan Region[J].Northern Horticulture,2020,44(07):36-42.[doi:10.11937/bfyy.20193520]
赣南地区部分脐橙品种对溃疡病的抗性
- Title:
- Resistance of Some Navel Orange Varieties to Canker Disease in Gannan Region
- Keywords:
- Gannan; navel orange; canker; resistance
- 文献标志码:
- A
- 摘要:
- 以多个脐橙优良品种为试材，采用离体、活体针刺法接种溃疡病菌，通过观察和测定菌斑数、叶片含水量、叶片丙二醛（MDA）含量等指标，研究同一砧木嫁接不同品种及不同砧木嫁接同一品种组合对溃疡病的抗病性,以期筛选出抗逆性较优的脐橙砧穗组合，并为赣南地区脐橙抗溃疡病种质资源的筛选与鉴定提供参考依据。结果表明：离体和活体针刺接种溃疡病菌的理想接种浓度约为8.4×107 cfu·mL-1；不同品种对比发现“纽荷尔”脐橙在接种溃疡病菌液后相对发病率最高，约为88%~96%，说明其对溃疡病的抗病性较弱；“华盛顿”脐橙相对发病率最低，约为36%~57%，说明其对溃疡病的抗病性较强；同一品种不同砧木组合对比，发现以枳壳作砧木的植株发病率均比以枳橙作砧木的高，表明以枳橙作砧木的植株耐受能力强于以枳壳作砧木的植株；柑橘对溃疡病的抗性与叶片含水量呈负相关，相关系数R2＝-0.948，与丙二醛含量呈负相关，相关系数R2＝-0.887。
- Abstract:
- Several navel orange cultivars were used as test materials to inoculate canker bacteria by acupuncture in vitro and in vivo,the resistance of different cultivars and different rootstock-scion combinations by observing incidence and exterminating physiological indexes were studied,in order to screen out navel orange rootstock-spike combinations with better stress resistance and provide reference basis for screening and identification of navel orange germplasm resources resistant to canker in Gannan region.The results showed that the ideal inoculation concentration was about 8.4×107 cfu·mL-1;The relative incidence of ‘Newhall’ navel orange was about 88%-96%,and the highest,it indicated that it had weak resistance to canker.‘Washington’navel orange had the lowest relative incidence,about 36%-57%,which showed that it had a strong resistance to canker disease;Compared with different rootstock-scion combinations,the incidence of citrange as rootstock was lower than that of trifoliate orange as rootstock,indicating that the tolerance of citrange as rootstock was stronger than that trifoliate orange as rootstock.The resistance of citrus to canker was negatively correlated with leaf water content,correlation coefficient was R2=-0.948;the resistance of citrus to canker was negatively correlated with the content of malondialdehyde (MDA),correlation coefficient was R2=-0.887.
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备注/Memo
第一作者简介：吕树萍（1992-），女，硕士研究生，研究方向为柑橘遗传育种。E-mail：1056998973@qq.com.责任作者：米兰芳(1982-)，女，硕士，讲师，研究方向为柑橘遗传育种。E-mail：mlfgnsy@163.com.基金项目：江西省科技成果转化资助项目（20171BEI90001）；市科技计划资助项目（赣市财教字[2016]15号）。收稿日期：2019-12-05