LI Xinhang,XIAO Zehua,LIU Wensheng.Physiological and Biochemical Responses of Kummerowia striata Seedlings in Heavy Metal Contaminated and Non-contaminated Areas to Mn Stress[J].Northern Horticulture,2020,44(14):118-128.[doi:10.11937/bfyy.20193503]
重金属污染区与非污染区鸡眼草幼苗对锰胁迫的生理生化响应
- Title:
- Physiological and Biochemical Responses of Kummerowia striata Seedlings in Heavy Metal Contaminated and Non-contaminated Areas to Mn Stress
- Keywords:
- manganese stress; heavy metal contaminated area; non-contaminated area; Kummerowia striata; seed germination; physiological and biochemical
- 文献标志码:
- A
- 摘要:
- 为探明鸡眼草（Kummerowia striata）对锰胁迫适应的理化机制，以重金属污染区（湖南湘潭锰矿）与非污染区（中南林业科技大学校园）的鸡眼草种子为研究对象，采用培养皿滤纸法，比较研究了不同浓度锰胁迫对2个居群种子发芽、幼苗生长及幼苗生理生化特性的影响。结果表明：1）随着锰浓度的升高，2个居群鸡眼草种子的发芽率、发芽势均呈逐渐降低的趋势，幼苗生长则呈现“低促高抑”现象，非污染区幼苗生长受到锰胁迫抑制作用高于污染区；2）随着锰浓度的升高，2个居群幼苗可溶性糖、可溶性蛋白质和脯氨酸含量均呈现先增加后下降的变化趋势；超氧化物歧化酶（SOD）活性基本无变化，过氧化物酶（POD）活性呈先增加后下降的变化趋势，丙二醛（MDA）含量则呈逐渐升高的趋势；3）根据抗锰隶属函数值的比较，鸡眼草对锰胁迫具有较强的耐性，污染区居群比非污染区居群具有更强的锰耐受性。
- Abstract:
- In order to find out the physical and chemical mechanism of adaptation of Kummerowia striata to manganese stress，the seeds of the scutellariae in the heavy metal contaminated area (Hunan Xiangtan Manganese Mine) and the non-contaminated area (Central South University of Forestry & Technology) were used as the research object.The effects of different concentrations of manganese stress on seed germination，seedling growth and physiological and biochemical characteristics of seedlings were studied.The results showed that,1)with the increase of manganese concentration，the germination rate and germination potential of the two populations of Chrysanthemum morifolium showed a decreasing trend，and the seedling growth showed a phenomenon of ‘low promotion and high inhibition’.The growth was inhibited by manganese stress higher than that of the contaminated area；2)with the increase of manganese concentration，the content of soluble sugar，soluble protein and proline in the two populations increased first and then decreased；the activity of SOD was basically unchanged，the activity POD increased first and then decreased，and the content of MDA gradually increased；3)according to the comparison of the values of the anti-manganese subordinate functions，the corn grass has strong tolerance to manganese stress，and the polluted area population has stronger manganese tolerance than the non-contaminated area.
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备注/Memo
第一作者简介：李欣航（1994-），女，硕士研究生，研究方向为土壤重金属污染修复。E-mail:453797699@qq.com.责任作者：刘文胜（1975-），男，博士，教授，现主要从事环境植物学等研究工作。E-mail：403493641@qq.com.基金项目：湖南省教育厅重点资助项目（14A156）；湖南省研究生科技创新资助项目（CX2017B415）。收稿日期：2019-11-28