[1]高娃,吴丰年,王妍晶,等.基于16S rDNA序列的柚喀木虱体内共生菌多样性研究[J].北方园艺,2020,44(03):22-28.[doi:10.11937/bfyy.20192185]
　GAO Wa,WU Fengnian,WANG Yanjing,et al.Study on the Endosymbionts Diversity in Cacopsylla citrisuga (Hemiptera:Psyllidae) Baseded on 16S rDNA Sequence[J].Northern Horticulture,2020,44(03):22-28.[doi:10.11937/bfyy.20192185]
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基于16S rDNA序列的柚喀木虱体内共生菌多样性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年03 页码: 22-28 栏目: 出版日期: 2020-02-15
Title:
Study on the Endosymbionts Diversity in Cacopsylla citrisuga (Hemiptera:Psyllidae) Baseded on 16S rDNA Sequence
作者:
高娃1; 吴丰年2; 王妍晶3; 徐长宝4; 岑伊静4
（1.包头市农牧业科学研究院,内蒙古 包头 014013；2.韩山师范学院 食品工程与生物科技学院，广东 潮州 521041；3.浙江农林大学 农业与食品学院，浙江 杭州 311300；4.华南农业大学 柑桔黄龙病研究室/广东省生物农药创制与应用重点实验室，广东 广州 510642）
Author(s):
GAO Wa1; WU Fengnian2; WANG Yanjing3; XU Changbao4; CEN Yijing4
(1.Baotou Academy of Agricultural and Animal Husbandry Sciences,Baotou,Inner Mongolia 014013;2.School of Food Engineering and Biotechnology,Hanshan Normal University,Chaozhou,Guangdong 521041;3.College of Agriculture and Food,Zhejiang A&F University,Hangzhou,Zhejiang 311300;4.Citrus Huanglongbing Research Laboratory/Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province,South China Agricultural University,Guangzhou,Guangdong 510642)
关键词:
柚喀木虱; 16S rDNA; RFLP; 体内共生菌; 多样性; 黄龙病
Keywords:
Cacopsylla citrisuga; 16S rDNA; RFLP; endosymbiont; diversity; huanglongbing
DOI:
10.11937/bfyy.20192185
文献标志码:
A
摘要:
柚喀木虱Cacopsylla citrisuga Yang&Li是柑桔黄龙病的传播媒介，在云南省宏德州主要为害柠檬、柚子等果树类的嫩梢及嫩叶，其成虫及若虫体内均检测到黄龙病亚洲种病原菌‘Candidates Liberibacter asiaticus’。该研究基于16S rDNA基因的RFLP指纹图谱法，建立了野外柠檬和柚子上柚喀木虱成虫体内微生物的16S rDNA文库，分析了柚喀木虱体内共生菌多样性。结果表明：柚喀木虱体内存在共9种体内共生菌，木虱/喀木虱次生共生菌（secondary endosymbiont of Psylla/secondary endosymbiont of Cacopsylla）、不可培养菌（uncultured bacterium）、Arsenophonus endosymbiont、Pantoea sp．、uncultured Erwinia sp．、Pseudomonas sp．、Rahnella sp．、Enterobacter sp．、Wolbachieae bacterium。根据指纹图谱结果分析，每个文库所分布操作单元（OUT）结果均不同，表明不同寄主植物及性别的柚喀木虱成虫体内共生菌的种类及数量存在差异。
Abstract:
Cacopsylla citrisuga Yang&Li was an insect vector of huanglongbing.This pest damaged the young leaves and flushes of lemon and pummel trees in Hongde Yunnan Province.A recent study indicated that both nymphs and adults of C.citrisuga carried ‘Candidates Liberibacter asiaticus’,the bacterium associated with huanglongbing.In this study,the endosymbiont 16S rDNA library of C.citrisuga collected from lemon and pomelo trees was built based on the RFLP of 16S rDNA gene,and the diversity of the endosymbionts was analyzed.The results showed that there were nine kinds of endosymbiont in this pest species,including secondary endosymbiont of Psylla/secondary endosymbiont of Cacopsylla,uncultured bacterium,Arsenophonus endosymbiont,Pantoea sp.,uncultured Erwinia sp.,Pseudomonas sp.,Rahnella sp.,Enterobacter sp.and Wolbachieae bacterium.According to the RFLP,each of the OUT was just different from others,indicated that species and quantity of the endosymbionts were different in C.citrisuga of different postplant and gender.

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备注/Memo

第一作者简介：高娃（1987-），女，硕士，助理农艺师，研究方向为植物保护。E-mail：gaowabao@163.com.责任作者：岑伊静（1966-），女，博士，副教授，硕士生导师，现主要从事昆虫生态学等研究工作。E-mail：cenyj@ scau.edu.cn.基金项目：国家重点研发计划资助项目（2018YFD0201502）；现代农业（柑桔）产业技术体系资助项目(CARS-27)。收稿日期：2019-09-17

更新日期/Last Update: 2020-02-26